Binding divergence of polystyrene nanoparticles with serum albumin caused by surface functionalization

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 903, P. 166578 - 166578

Published: Aug. 25, 2023

Language: Английский

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Mechanisms of eco-corona effects on micro(nano)plastics in marine medaka: Insights into translocation, immunity, and energy metabolism

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136236 - 136236

Published: Oct. 21, 2024

Language: Английский

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Co-Existing Nanoplastics Further Exacerbates the Effects of Triclosan on the Physiological Functions of Human Serum Albumin

Life, Journal Year: 2025, Volume and Issue: 15(1), P. 112 - 112

Published: Jan. 16, 2025

The potential health risks posed by the coexistence of nanoplastics (NPs) and triclosan (TCS) have garnered significant attention. However, effects underlying mechanisms NPs TCS on key functional proteins at molecular level remain poorly understood. This study reports effect polystyrene (PSNPs) binding to human serum albumin (HSA) using multispectral methods simulation systems. experimental results show that significantly inhibits HSA esterase activity, with exacerbating inhibition in presence PSNPs, which is attributed alteration conformation microenvironment amino acid residues induced PSNPs. Molecular docking site marker competitive studies indicate predominantly binds I subdomain Sudlow II PSNPs does not affect sites. Spectra analyses quenching mechanism between belongs static type change fluorescence type. conformational alterations are further enhanced indicating enhance making more accessible provides valuable information about toxicity case co-exposure.

Language: Английский

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Exposure of Polystyrene Micro- and Nanoplastics to Simulated Human Digestive Enzymatic Systems: Structural and Functional Implications

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

The current in vitro study explores the exposure of emerging pollutants polystyrene micro- and nanoplastics (PS-MNPs) within digestive system their interaction with key enzymes such as α-amylase, pepsin, pancreatin. present research aims to elucidate potential health implications by PS-MNPs based on previously estimated mean ingested microplastics (MPs) (0.714 g/day). deepens our understanding environmental pollutants' impact human examining interactions between (PS) (PS MPs, 37-50 μm approx.) PS NPs, 100 nm) enzymes. analyzes effects nanosized plastics enzyme activity using multiple spectroscopic techniques, revealing molecular mechanisms inhibition structural changes caused more than those MPs. fluorescence emission spectra indicated a static quenching mechanism across all at K q = 3.638, 4.615, 1.855 (∼× 1018 M-1·s-1), predominantly affecting tyrosine (Tyr) tryptophan (Trp) residues. Resonance light scattering (RLS) confirmed formation enzyme-PS NPs complexes, leading aggregation. Fourier transform infrared (FT-IR) circular dichroism (CD) spectrometry results showed decrease protein content alterations enzymes, potentially function. half inhibitory concentration (IC50) values for salivary α-amylase (180 μg/mL), pepsin (580 pancreatic protease (314 μg/mL) indicate uncompetitive inhibition, that (592 indicates mixed reversible highlights risks associated gives broader interplay plastic health.

Language: Английский

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Effects of Environmental Pollutants on Tryptophan Metabolism

Toxics, Journal Year: 2025, Volume and Issue: 13(4), P. 311 - 311

Published: April 17, 2025

Tryptophan (Trp) is an important essential amino acid that plays a variety of physiological functions in the human body, including being precursor neurotransmitter and participating immune regulation. Currently, more studies show some pollutants environment can affect metabolism Trp consequently health. The present paper offers comprehensive overview prior research investigating impact environmental pollutants, inorganic organic contaminants, microplastics, nanoplastics on nervous system, digestive maternal–fetal pregnancy, revealing their detrimental effects well-being.

Language: Английский

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Uncovering the Interaction of 2‐(4‐Aminophenyl) benzothiazole with Human Serum Albumin: A Combined Spectroscopic and Docking Study

ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(16)

Published: April 1, 2025

Abstract Drug interactions with receptors determine their biological activity. Among the proteins, human serum albumin (HSA) is most commonly used as model protein to explore such interactions. The heterocyclic molecule, 2‐(4‐aminophenyl) benzothiazole (APB), known have various potential. binding of APB HSA has been presented by looking into parameters and effect on structural aspect protein. constant (K b ), number sites (n), quenching SV bimolecular (k q ) estimated from experimental spectroscopic methods. was found be in order 10 5 M −1 . Quenching fluorescence at 303, 308, 313 K showed a dynamic nature quenching. spontaneous negative free energy change (ΔG 0 ). binding's enthalpy (ΔH0) entropy (ΔS0) changes suggested significant role electrostatic force. Site‐specific marker displacement studies revealed subdomain IIA. Circular dichroism (CD) no visible HSA. finding corroborated molecular docking that location This study may help understand its analogues or related structures aid designing molecules better

Language: Английский

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Size-Dependent Interactions of Degraded PET Nanoparticles with Human Serum Albumin: Thermodynamic and Molecular Insights

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

This study examines the interaction between degraded polyethylene terephthalate (PET) nanoparticles and human serum albumin (HSA), focusing on effects of nanoparticle size surface modifications resulting from degradation. PET degradation, induced via shock compression in water, leads to significant chemical alterations, including formation hydroxyl, carboxyl, carbonyl groups. These influence hydrophilicity their binding behavior with HSA. The production involves subjecting pristine controlled an aqueous environment, which initiates reactions similar those that may occur during degradation process is characterized by a progressive breakdown polymer chains, leading increase functionalized groups enhanced hydrophilicity. performed analysis chemistry reveals introduction oxygen-containing alters properties nanoparticles, making them more prone hydrogen bonding water molecules while simultaneously reducing affinity for HSA binding. Molecular dynamics simulations, umbrella sampling, weighted histogram are employed investigate thermodynamic aspects PET-HSA interactions. identifies preferred sites HSA, revealing preferentially bind Domain I III Interaction energy demonstrates larger exhibit stronger binding, whereas small have significantly reduced energies, indicating higher likelihood desorption. Further structural using root-mean-squared deviation (RMSD) fluctuation (RMSF) confirms does not alter HSA's secondary structure. However, increases hydrophilicity, weakening adsorption onto Large strongly bound, remain unbound, raising concerns regarding potential toxicity due free migration bloodstream. findings provide crucial insights into biological implications role determining interactions, contributions nanoplastic toxicity.

Language: Английский

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Complementary Analysis for Undetectable Microplastics from Contact Lenses to Aquatic Environments via Fourier Transform Infrared Spectroscopy

Molecules, Journal Year: 2023, Volume and Issue: 28(9), P. 3713 - 3713

Published: April 25, 2023

Although microplastics (MPs) are intrinsically toxic and function as vectors for organic micropollutants, their discharge from wastewater treatment plant effluents human activity remains unknown owing to the limitations of detection technologies. It is imperative quantify MPs activities involving consumption various plastic products. This study warns that contact lenses can generate nanoplastics (NPs) after being discharged into aquatic environments. Identification via micro-Fourier transform infrared spectroscopy revealed fragmented particles (from a few tens hundred micrometres) could not be detected poly(2-hydroxyl methacrylate), component lenses, changes in its chemical properties. After degradation process, median size lens decreased 313 85 µm. Approximately 300,600 g waste sewage systems daily United States America (USA), where 45 million people wear throw away one-fifth them every day. Contact (1 g) has potential release 5653.3-17,773.3 MPs. implies currently reported MP amounts environmental matrix exclude significant NPs lenses. The identification method should examined, registration disposal process established.

Language: Английский

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Exposure to phagolysosomal simulated fluid altered the cytotoxicity of PET micro(nano)plastics to human lung epithelial cells

Toxicology Mechanisms and Methods, Journal Year: 2023, Volume and Issue: 34(1), P. 72 - 97

Published: Sept. 12, 2023

The occurrence of micro(nano)plastics into various environmental and biological settings influences their physicochemical toxic behavior. Simulated body fluids are appropriate media for understanding the degradation, stability, interaction with other substances any material in human body. When particles enter via inhalation, which is one avenues micro(nano)plastics, they first come contact lung lining fluid under neutral conditions then phagocytosed acidic to be removed. Therefore, it important examine transformation toxicity characteristics after phagolysosomal simulant (PSF). Here, we focused on exploring how differences (e.g. surface chemistry, elemental distribution, charge) pH 4.5 phagolysosome impact cytotoxicity oxidative epithelia cells. cells those treated PSF non-treated was tested by viability indicators including cell counting kit-8 (CCK-8), MTT, LDH. Furthermore, background examined through processes reactive oxygen species, antioxidant, superoxide dismutase (SOD), catalase, reduced glutathione). results showed that all were significantly influenced conditions. staged responses observed treatment duration, significant changes calculated carbonyl, carbon-nitrogen, sulfonyl groups. Moreover, negativity zeta potentials declined between exposure 2-40 h increased at 80 compared control owing chemical functional groups distribution plastic particles. cytotoxic SOD dominant enzyme triggering due particle degradation instability.

Language: Английский

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Adsorption of clarithromycin on polystyrene nanoplastics surface and its combined adverse effect on serum albumin

Colloids and Surfaces B Biointerfaces, Journal Year: 2023, Volume and Issue: 234, P. 113673 - 113673

Published: Dec. 1, 2023

Language: Английский

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