Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Dec. 28, 2024
To
investigate
the
structural
and
functional
similarities
of
microbial
communities
in
burnt-sweetness
alcoholized
tobacco
as
a
function
distance
from
equator
their
effects
on
quality,
we
sampled
Chenzhou,
Hunan
Province,
China
Brazil
Zimbabwe,
which
are
also
burnt-sweetness-type
producing
regions,
performed
high-throughput
sequencing
bacterial
fungal
along
with
an
analysis
main
chemical
constituents
to
analyze
differences
quality
structure
communities.
The
total
nitrogen,
nicotine
starch
contents
Chenzhou
were
greater
than
those
Brazilian
Zimbabwean
tobacco,
sugar
reducing
(P
<
0.05).
alpha
diversity
indices
lower
In
ecological
networks,
bacterial–fungal
interactions
more
complex
networks
three
different
regions
dominated
by
competitive
relationships.
community
composition
was
similar
that
at
genus
phylum
level,
Sphingomonas
being
significantly
enriched
key
network
is
able
participate
degradation
polyphenols
aromatic
compounds.
Functional
microbes
related
compounds
cellulose
abundant
substances
responsible
for
better
tobacco.
conclusion,
there
structure,
flora
because
these
have
latitudinal
distributions.
This
study
provides
theoretical
support
selecting
cultivation
alcoholization
leaves.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Sept. 17, 2024
Introduction
Carotenoids
are
important
precursors
of
various
aroma
components
in
tobacco
and
play
an
role
the
sensory
quality
tobacco.
Phaffia
rhodozyma
is
a
species
Xanthophyllomyces
capable
synthesizing
highly
valuable
carotenoid-astaxanthin,
but
has
not
yet
been
used
improving
quality.
Methods
The
dynamic
changes
microbial
community
metabolites
during
fermentation
were
analyzed
combination
with
microbiome
metabolome,
after
was
evaluated
by
scores.
Results
P.
could
grow
produce
carotenoids
extract,
maximum
biomass
6.50
g/L
carotenoid
production
36.13
mg/L
at
100
extract.
Meanwhile,
correlation
analysis
combined
metabolomics
showed
that
significantly
positively
correlated
11
such
as
6-hydroxyluteolin
quercetin.
Furthermore,
contents
alcohols,
ketones
esters,
which
aromatic
fermented
tobacco,
reached
77.57
μg/g,
58.28
μg/g
73.51
increasing
37.39%,
265.39%
266.27%
compared
to
control
group,
respectively.
Therefore,
flavor,
taste
scores
increased
0.5
1.0
points
Discussion
This
study
confirmed
effectively
improve
evaluation
provided
novel
method
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 26, 2024
Abstract
To
investigate
the
structural
and
functional
similarities
of
microbial
communities
in
burnt-sweetness
alcoholized
tobacco
as
a
function
distance
from
equator
their
effects
on
quality,
we
sampled
Chenzhou,
Hunan
Province,
China
Brazil
Zimbabwe,
which
are
also
burnt-sweetness-type
producing
regions,
performed
high-throughput
sequencing
bacterial
fungal
along
with
an
analysis
main
chemical
constituents
to
analyze
differences
quality
structure
communities.
The
total
nitrogen,
nicotine
starch
contents
Chenzhou
were
greater
than
those
Brazilian
Zimbabwean
tobacco,
sugar
reducing
(P
<
0.05).
alpha
diversity
indices
lower
In
ecological
networks,
bacterial–fungal
interactions
more
complex
networks
three
different
regions
dominated
by
competitive
relationships.
community
composition
was
similar
that
at
genus
phylum
level,
Sphingomonas
being
significantly
enriched
key
network
is
able
participate
degradation
polyphenols
aromatic
compounds.
Functional
microbes
related
compounds
cellulose
abundant
substances
responsible
for
better
tobacco.
conclusion,
there
structure,
flora
because
these
have
latitudinal
distributions.
This
study
provides
theoretical
support
selecting
cultivation
alcoholization
leaves.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Nov. 14, 2024
Objective
This
study
aims
to
investigate
the
effects
of
pectin-degrading
bacteria
on
microbial
community
and
physicochemical
properties
during
fermentation
process
cigar
tobacco,
evaluating
its
potential
in
reducing
green
bitterness
enhancing
aroma.
Methods
By
isolating
screening
bacteria,
high-throughput
sequencing
analysis
were
employed
compare
flora
component
differences
different
treatment
groups
tobacco.
Furthermore,
correlation
was
performed
examine
relationships
between
these
variables.
Results
The
results
showed
that
strains
YX-2
DM-3,
isolated
from
tobacco
variety
“
Yunxue
No.
1
,”
exhibited
strong
abilities.
Phylogenetic
revealed
strain
is
highly
homologous
Bacillus
flexus
,
while
DM-3
siamensis
.
After
fermentation,
addition
significantly
reduced
pectin
content
leaves,
increased
total
sugar
content,
bitterness,
markedly
enhanced
aroma
components.
Notably,
outstanding
performance
production
Maillard
reaction
products.
Microbial
diversity
both
fungi,
especially
TDM3
group,
where
relative
abundance
Pseudomonas
notably
elevated.
Correlation
had
a
significant
positive
with
sugar,
negative
pectin,
indicating
important
role
metabolism
degradation.
Additionally,
fungal
genera
such
as
Cercospora
negatively
correlated
nitrogen,
Eurotium
closely
associated
degradation
accumulation.
Conclusion
found
Pectin-degrading
optimized
structure
improved
notable
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Dec. 28, 2024
To
investigate
the
structural
and
functional
similarities
of
microbial
communities
in
burnt-sweetness
alcoholized
tobacco
as
a
function
distance
from
equator
their
effects
on
quality,
we
sampled
Chenzhou,
Hunan
Province,
China
Brazil
Zimbabwe,
which
are
also
burnt-sweetness-type
producing
regions,
performed
high-throughput
sequencing
bacterial
fungal
along
with
an
analysis
main
chemical
constituents
to
analyze
differences
quality
structure
communities.
The
total
nitrogen,
nicotine
starch
contents
Chenzhou
were
greater
than
those
Brazilian
Zimbabwean
tobacco,
sugar
reducing
(P
<
0.05).
alpha
diversity
indices
lower
In
ecological
networks,
bacterial–fungal
interactions
more
complex
networks
three
different
regions
dominated
by
competitive
relationships.
community
composition
was
similar
that
at
genus
phylum
level,
Sphingomonas
being
significantly
enriched
key
network
is
able
participate
degradation
polyphenols
aromatic
compounds.
Functional
microbes
related
compounds
cellulose
abundant
substances
responsible
for
better
tobacco.
conclusion,
there
structure,
flora
because
these
have
latitudinal
distributions.
This
study
provides
theoretical
support
selecting
cultivation
alcoholization
leaves.
