Role of an endodermis‐specific miR858b‐MYB1L module in the regulation of Taxol biosynthesis in Taxus mairei

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)

Published: April 1, 2025

SUMMARY Taxol, a chemotherapeutic agent widely used for treating various cancers, is extracted from the stems of Taxus mairei . However, current knowledge regarding effects stem tissue and age on Taxol accumulation limited. We employed matrix‐assisted laser desorption/ionization mass spectrometry to visualize taxoids in section sections varying ages T. Laser capture microdissection integrated with data‐dependent acquisition–MS/MS analysis identified that several biosynthesis pathway‐related enzymes were predominantly produced endodermis, elucidating molecular mechanisms underlying endodermis‐specific accumulation. an MYB1‐like (MYB1L) protein proposed potential function miR858‐MYB1L module regulating secondary metabolic pathways. DNA affinity purification sequencing 92 506 target peaks MYB1L. Motif enrichment de novo motifs, providing new insights into MYB recognition sites. Four MYB1L within promoter sequences synthesis genes, including TBT , DBTNBT T13OH BAPT confirmed using electrophoretic mobility shift assays. Dual‐luciferase assays showed significantly activated expression Our data indicate miR858b‐MYB1L plays crucial role transcriptional regulation by up‐regulating genes endodermis.

Language: Английский

Metabolic and transcriptomic responses of <i>Taxus mairei</i> to nano-pollutants: insights into AgNPs and PsNPs impact

Medicinal Plant Biology, Journal Year: 2025, Volume and Issue: 4(1), P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Single-cell transcriptional profiling reveals cell type-specific responses to duck reovirus infection in the Bursa of Fabricius of Cairna moschata

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 136391 - 136391

Published: Oct. 1, 2024

Language: Английский