Selection of suitable reference lncRNAs for gene expression analysis in Osmanthus fragrans under abiotic stresses, hormone treatments, and metal ion treatments

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 21, 2025

Intoduction Osmanthus fragrans , a well-regarded traditional flower in China, holds extensive applications gardening, food, cosmetics, and Chinese medicine. Despite its importance, research on long non-coding RNAs (lncRNAs) O. has been constrained by the absence of reliable reference genes (RGs). Methods We employed five distinct algorithms, i.e., delta-Ct, NormFinder, geNorm, BestKeeper, RefFinder, to evaluate expression stability 17 candidate RGs across various experimental conditions. Results discussion The results indicated most stable RG combinations under different conditions as follows: cold stress: lnc00249739 lnc00042194; drought lnc00042194 lnc00174850; salt lnc00239991 abiotic lnc00239991, lnc00042194, lnc00067193, lnc00265419; ABA treatment: 18S ; MeJA lnc00265419 lnc00249739; ethephon lnc00229717 lnc00044331; hormone treatments: lnc00239991; Al 3+ lnc00087780 Cu 2+ lnc00067193 Fe ACT7 metal ion lnc00067193; flowering stage: RAN1 tissues: TUA5 UBQ4 all samples: . reliability these selected was further validated analyzing patterns lnc00003036, lnc00126603, lnc00250780. This study represents first comprehensive evaluation lncRNA significantly enhancing accuracy depth analyses this species, contributing advancements plant stress resistance breeding improving environmental adaptability.

Language: Английский

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Transcriptomic Analysis of Broussonetia papyrifera Fruit Under Manganese Stress and Mining of Flavonoid Synthesis Genes

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 883 - 883

Published: March 12, 2025

Broussonetia papyrifera is a deciduous tree with significant economic and medicinal value. It demonstrates notable physiological adaptability to mining areas severe manganese contamination pioneering species in the field of ecological restoration. Flavonoids are vital secondary metabolites that improve plant resilience environmental stresses. In study presented herein, immature mature fruits B. grown normal high environments were used as test materials. fruit was subjected transcriptome sequencing via high-throughput technology analyze its flavonoid metabolic pathways related genes. Transcriptome identified total 46,072 unigenes, an average length 1248 bp percentage Q30 bases ranging from 92.45 93.17%. Furthermore, 31,792 unigenes (69% total) annotated using eight databases, including GO KEGG. Analysis KEGG content trends revealed four strong links biosynthesis pathway under stress: flavone 3-hydroxylase, flavonoids 3′,5′-O-methyltransferase, chalcone synthase, flavonol synthase. These may play important roles regulating synthesis stress. This lays groundwork for functional gene research papyrifera.

Language: Английский

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An integrative overview of cold response and regulatory pathways in horticultural crops

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

ABSTRACT Global climate change challenges agricultural production, as extreme temperature fluctuations negatively affect crop growth and yield. Low (LT) stress impedes photosynthesis, disrupts metabolic processes, compromises the integrity of cell membranes, ultimately resulting in diminished yield quality. Notably, many tropical or subtropical horticultural plants are particularly susceptible to LT stress. To address these challenges, it is imperative understand mechanisms underlying cold tolerance crops. This review summarizes recent advances physiological molecular that enable crops withstand stress, emphasizing discrepancies between model systems. These include C‐repeat binding factor‐dependent transcriptional regulation, post‐translational modifications, epigenetic control, regulation. Reactive oxygen species, plant hormones, light signaling pathways integrated into response network. Furthermore, technical for improving highlighted, including genetic improvement, application light‐emitting diodes, utility novel regulators, grafting. Finally, prospective directions fundamental research practical applications boost discussed.

Language: Английский

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Comprehensive transcriptome analysis of AP2/ERFs in Osmanthus fragrans reveals the role of OfERF017-mediated organic acid metabolism pathway in flower senescence

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 26, 2024

Osmanthus fragrans is an ethylene-sensitive flower, and flower senescence was mediated by ethylene-responsive transcription factors ( OfERFs ). A total of 227 were identified from O. , which classified into five subfamilies: AP2 (35), DREB (57), ERF (125), RAV (6), Soloist (4). Gene composition structural analysis indicate that members different subfamilies have gene structures conserved domains. Their promoter contains various functional responsive elements, including auxin, jasmonic acid, other elements. Among them, 124 OfAP2/ERF genes expressed at any stage flowering, 10 them may play roles in flowering or senescence. By comparative transcriptome analysis, OfAP2/ERFs affected ethephon (ETH) 5′-azacytidine (Aza) treatment divided three categories, target sets. Importantly, these sets participate similar biological processes metabolic pathways, suggesting ethylene DNA hypomethylation crosstalk a unique mechanism regulating the fragrans. Co-expression revealed several key played central role organic acid metabolism biosynthesis branched-chain amino acids (BcAAs), among OfERF017 selected for further analysis. Overexpression leads to significant enrichment decrease levels promoting . Together, results give insights characteristics

Language: Английский

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