Hybridoma technology: is it still useful?

Current Research in Immunology, Journal Year: 2021, Volume and Issue: 2, P. 32 - 40

Published: Jan. 1, 2021

The isolation of single monoclonal antibodies (mAbs) against a given antigen was only possible with the introduction hybridoma technology, which is based on fusion specific B lymphocytes myeloma cells. Since then, several mAbs were described for therapeutic, diagnostic, and research purposes. Despite being an old technique low complexity, hybridoma-based strategies have limitations that include efficiency lymphocyte-myeloma cell step, need to use experimental animals. In face that, methods been developed improve mAb generation, ranging from changes in advent completely new technologies, such as antibody phage display ones. this review, we discuss technology along emerging approaches, taking into account their advantages limitations. Finally, explore usefulness nowadays.

Language: Английский

---

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Journal of Natural Medicines, Journal Year: 2022, Volume and Issue: 76(3), P. 521 - 545

Published: Feb. 16, 2022

Abstract Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality plant samples perspective efficacy by measuring necessary secondary metabolites and safety adulterant level toxic compounds. The development reliable detection methods for these such a complicated matrix is challenging. lateral flow immunoassay (LFA) one immunoassays well-known its simplicity, portability, rapidity. In this review, general principle, components, format, application LFA phytoproducts discussed.

Language: Английский

---

In vivo Phage Display: A promising selection strategy for the improvement of antibody targeting and drug delivery properties

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 26, 2022

The discovery of hybridoma technology, described by Kohler and Milstein in 1975, the resulting ability to generate monoclonal antibodies (mAbs) initiated a new era antibody research clinical development. However, limitations technology as routine generation method conjunction with high immunogenicity responses have led development alternative approaches for streamlined identification most effective antibodies. Within this context, display selection technologies such phage display, ribosome yeast bacterial mammalian cell surface been widely promoted over past three decades ideal alternatives traditional methods. on phages is probably widespread powerful these methods and, since its invention late 1980s, significant technological advancements design, construction, libraries made, several fully human generated are currently approved or various stages. With evolving novel disease targets emerging therapeutic antibodies, bispecific drug conjugates (ADCs), chimeric antigen receptor T (CAR

Language: Английский

---

Antibody-receptor bioengineering and its implications in designing bioelectronic devices

International Journal of Biological Macromolecules, Journal Year: 2022, Volume and Issue: 218, P. 225 - 242

Published: July 20, 2022

Language: Английский

---

Recent advances in culture medium design for enhanced production of monoclonal antibodies in CHO cells: A comparative study of machine learning and systems biology approaches

Biotechnology Advances, Journal Year: 2024, Volume and Issue: unknown, P. 108480 - 108480

Published: Nov. 1, 2024

Language: Английский

---