Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 16, 2023

Decellularization of tissues and organs has recently become a promising approach in tissue engineering regenerative medicine to circumvent the challenges organ donation complications transplantations. However, one main obstacle reaching this goal is acellular vasculature angiogenesis endothelialization. Achieving an intact functional vascular structure as vital pathway for supplying oxygen nutrients remains decisive challenge decellularization/re-endothelialization procedure. In order better understand overcome issue, complete appropriate knowledge endothelialization its determining variables required. methods their effectiveness, biological mechanical characteristics scaffolds, artificial bioreactors, possible applications, extracellular matrix surface modification, different types utilized cells are factors affecting consequences. This review focuses on how optimize them, well discussing recent developments process re-endothelialization.

Language: Английский

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Antimicrobial Activity of Dimeric Flavonoids

Compounds, Journal Year: 2024, Volume and Issue: 4(2), P. 214 - 229

Published: March 22, 2024

Distributed throughout the environment are various microorganisms such as bacteria, fungi, parasites, and viruses. Although many part of human microbiome, pathogenic cause infections ranging from mild to severe. In recent years, identification multidrug-resistant has become a serious public health problem. The resulting call into question therapeutic capacity systems lead approximately 70,000 deaths annually worldwide. progressive resistance antibiotics antifungals been major challenge for medical pharmaceutical community, requiring search new compounds with antimicrobial properties. Several studies have demonstrated potential natural synthesized flavonoids, especially dimers these molecules. this review presented examples dimeric flavonoids that activity against viruses, like influenza Human Immunodeficiency Virus (HIV), protozoal infections, Leishmaniasis Malaria, fungal by Candida albicans Cryptococcus neoformans, bacterial caused, example, Staphylococcus aureus Escherichia coli. pursuit find safe agents therapy in microbial an option not only activity, but also low toxicity usually associated when compared classic antimicrobials.

Language: Английский

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Increasing plasma calprotectin (S100A8/A9) is associated with 12-month mortality and unfavourable functional outcome in critically ill COVID-19 patients

Journal of Intensive Care, Journal Year: 2024, Volume and Issue: 12(1)

Published: July 9, 2024

Abstract Background Calprotectin (S100A8/A9) is a pro-inflammatory mediator primarily released from neutrophils. Previous studies have revealed associations between plasma calprotectin, disease severity and in-hospital mortality in unselected COVID-19 patients. Objective We aimed to assess whether calprotectin dynamics during the first week of intensive care are associated with functional outcome critically ill Methods This prospective study included 498 patients admitted six units (ICUs) Sweden May 2020 2021. Blood samples were collected on ICU admission day 7. The primary was 12-month mortality. Secondary outcomes survivors at 3 12 months, need for invasive mechanical ventilation (IMV) or continuous renal replacement therapy (CRRT) stay. Functional assessed by Glasgow Outcome Scale Extended (GOSE, range 1–8, < 5 representing an unfavourable outcome). Associations examined binary logistic regression analyses adjusted age, sex, BMI, hypertension, smoking, creatinine. Results High 7 independently increased Increasing higher months [OR 2.10 (95% CI 1.18–3.74), p = 0.012], 2.53 1.07–6.10), 0.036], use IMV 2.23 1.10–4.53), 0.027)] CRRT 2.07 1.07–4.00), 0.031)]. A receiver operator characteristic (ROC) model including age good predictor [AUC 0.79 0.74–0.84), 0.001]. Day alone predicted 0.67 0.58–0.76), Conclusion In patients, increasing levels after survivors. Monitoring may be considered evaluate prognosis critical COVID-19. Study registration: ClinicalTrials.gov Identifier : NCT04974775, registered April 28, 2020.

Language: Английский

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COVID-19 reduces cardiorespiratory fitness even months after a mild to moderate acute phase: a retrospective cohort study

Infectious Diseases, Journal Year: 2023, Volume and Issue: 55(10), P. 684 - 693

Published: July 3, 2023

Background COVID-19 survivors may experience long-term health problems, including deterioration of cardiorespiratory fitness (CRF), as demonstrated by several cross-sectional studies that compared the results cardiopulmonary exercise tests (CPET) performed only after with predicted values. This study aimed to analyze a change in CRF between repeated CPETs response suffered COVID-19.Methods A total 127 healthcare workers (HCWs; mean age 55.7 years) underwent two interval 762 days. Forty HCWs from (mild moderate severity) interim (321 days before second CPET), and 87 formed control group. Mixed-effects regression multiple adjustment interaction terms was used for variables – maximum oxygen uptake (VO2 max) power output.Results Between both CPETs, VO2 max decreased statistically significantly subgroup (by 3.12 mL/kg/min, p = .034) insignificantly controls 0.56 .412). The proportion achieving 75.9% 59.5% (p .161) survivors, while it increased 73.8% 81% .274) controls. (β −0.66, .014) body mass index −0.49, < .001) were independent negative predictors change. not associated output.Conclusions On basis significantly, albeit rather modestly, reduces almost one year infection. reduction persists even acute phase mild or severity.

Language: Английский

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Chronic hyperglycemia impairs anti-microbial function of macrophages in response to Mycobacterium tuberculosis infection

Immunologic Research, Journal Year: 2024, Volume and Issue: 72(4), P. 644 - 653

Published: Feb. 12, 2024

Language: Английский

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COVID-19 and immune dysfunction: Consequences and natural remedies for management

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 235 - 264

Published: Jan. 1, 2025

Language: Английский

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Post-pandemic insights on COVID-19 and premature ovarian insufficiency

Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

The COVID-19 pandemic has raised concerns regarding its potential impact on premature ovarian insufficiency (POI). This overview examines the possible interactions between and POI, while also suggesting preventive measures. viral infection's inflammatory response immune dysregulation may adversely affect tissues, leading to inflammation damage. Additionally, alterations in vascular function could impair blood flow hormonal imbalances disrupt normal function. Long-term health effects, such as "long COVID," exacerbate these issues through chronic dysfunction. Public measures, vaccination home isolation, indirectly protect by reducing systemic inflammation. Vaccines mitigate severity of COVID-19's function, isolation reduce stress However, further research is needed validate mechanisms.

Language: Английский

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Dissecting the COVID‐19 Immune Response: Unraveling the Pathways of Innate Sensing and Response to SARS‐CoV‐2 Structural Proteins

Journal of Molecular Recognition, Journal Year: 2025, Volume and Issue: 38(2)

Published: Feb. 5, 2025

ABSTRACT Severe acute respiratory syndrome coronavirus (SARS‐CoV), the virus responsible for COVID‐19, interacts with host immune system through complex mechanisms that significantly influence disease outcomes, affecting both innate and adaptive immunity. These interactions are crucial in determining disease's severity host's ability to clear virus. Given virus's substantial socioeconomic impact, high morbidity mortality rates, public health importance, understanding these is essential. This article examines diverse responses triggered by SARS‐CoV‐2's structural proteins, including spike (S), membrane (M), envelope (E), nucleocapsid (N) along nonstructural proteins (NSPs) open reading frames. play pivotal roles modulation, facilitating viral replication, evading detection, contributing severe inflammatory such as cytokine storms distress (ARDS). The employs strategies like suppressing type I interferon production disrupting key antiviral pathways, MAVS, OAS‐RNase‐L, PKR. study also explores pathways govern activation suppression of throughout COVID‐19. By analyzing sensing receptors initiated upon recognizing SARS‐CoV‐2 this review elucidates associated response Understanding offers valuable insights therapeutic interventions informs strategies, a deeper COVID‐19 immunopathogenesis.

Language: Английский

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COVID-19 beyond the lungs: Unraveling its vascular impact and cardiovascular complications—mechanisms and therapeutic implications

Science Progress, Journal Year: 2025, Volume and Issue: 108(1)

Published: Jan. 1, 2025

COVID-19, caused by severe acute respiratory syndrome corona virus 2 (SARS-CoV-2), is primarily a illness but significantly affects the cardiovascular system as well. After entering body through tract, directly and indirectly disrupts vascular system. Vascular endothelial cells (ECs), which express ACE2 TMPRSS2, are targets for viral invasion. However, predominant cause of widespread damage “cytokine storm” induced immune response. This leads to EC activation, inflammation, neutrophil neutrophil–platelet aggregation, causing injury. Additionally, increased expression plasminogen activator inhibitor-1 balance between prothrombotic fibrinolytic processes, while activation renin–angiotensin–aldosterone adds oxidative stress endothelium. In heart, SARS-CoV-2 invades ECs, leading apoptosis pyroptosis, exacerbated inflammation elevated catecholamines. These factors contribute arrhythmias, strokes, myocardial infarction in cases COVID-19. narrative review aims explore mechanisms highlight resulting complications. It also identifies research gaps discusses potential therapeutic strategies mitigate impacts

Language: Английский

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The immuno-metabolic trade-off: a driver of metabolic disorders and aging

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

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