Comprehensive analytical chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Language: Английский
Neuron, Journal Year: 2025, Volume and Issue: 113(5), P. 684 - 700.e8
Published: Jan. 13, 2025
Spinal cord injury (SCI) increasingly affects aged individuals, where functional impairment and mortality are highest. However, the aging-dependent mechanisms underpinning tissue damage remain elusive. Here, we find that natural killer-like T (NKLT) cells seed intact human murine spinal multiply further after injury. NKLT accumulate in via C-X-C motif chemokine receptor 6 ligand 16 signaling to clonally expand by engaging with major histocompatibility complex (MHC)-I-expressing myeloid cells. expressing killer cell granule protein 7 (Nkg7) disrupt myeloid-cell-dependent wound healing injured cord. Nkg7 deletion mice curbs degranulation normalize phenotype, thus promoting repair axonal integrity. Monoclonal antibodies neutralizing CD8+ SCI enhance neurological recovery healing. Our results unveil a reversible role for NKG7+CD8+ exacerbating damage, suggesting clinically relevant treatment SCI.
Language: Английский
Citations
2
BBA Advances, Journal Year: 2025, Volume and Issue: 7, P. 100149 - 100149
Published: Jan. 1, 2025
The fundamental biological processes involving highly specific interactions between proteins and other motifs are the pillars of protein recognition mechanisms. These crucial for systems, often having significant implications within diagnostics therapy development. Protein specificity reliant on structural compatibility, dynamic conformational changes, biochemical interactions-all which grounded in molecular forces like hydrogen bonding, ionic interactions, van der Waals forces. Advanced characterization tools have improved our understanding revealing kinetics thermodynamics these In parallel, new computing methods, including artificial intelligence, docking, dynamical simulations, increased prediction accuracy leading to well-defined interaction sites binding information. is pivotal diagnostic methods ELISAs biosensors, disease detection applications. therapeutics, plays an important role drug development, enabling design small molecules, peptides, monoclonal antibodies. Despite recent progress, there many challenges remaining fully understand recognition, particularly complex cell environment. require future work studies enhance therapeutic researchers using screening identify, assess, optimize clinical translation.
Language: Английский
Citations
1
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: Dec. 1, 2023
Recent progressions in immunotherapy have transformed cancer treatment, providing a promising strategy that activates the immune system of patient to find and eliminate cancerous cells. Bispecific antibodies, which engage two separate antigens or one antigen with distinct epitopes, are tremendous concern immunotherapy. The bi-targeting idea enabled by bispecific antibodies (BsAbs) is especially attractive from medical standpoint since most diseases complex, involving several receptors, ligands, signaling pathways. Several research look into processes BsAbs identify different targets such angiogenesis, reproduction, metastasis, regulation. By rerouting cells altering other pathways, proteins perform effector activities addition those natural antibodies. This opens up wide range clinical applications helps patients resistant tumors respond better medication. Yet, further study necessary best conditions where use these medications for treating tumor, their appropriate combination partners, methods reduce toxicity. In this review, we provide insights BsAb format classification based on composition symmetry, as well delivery mode, focus action mechanism molecule, discuss challenges future perspectives development.
Language: Английский
Citations
15
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 11, 2025
The direct and precise assessment of ligand–protein interactions under nearly physiological conditions is the core drug discovery. In this context, affinity capillary electrophoresis (ACE) has become an emerging reliable approach. hyphenation ACE with mass spectrometry (MS) even more powerful than classical ACE-UV methodology. It reduces compound identification errors increases throughput by facilitating analysis mixtures. However, buffers coatings compatible operating are very limited. paper, n-methylmorpholine acetate buffer polydopamine-based coating were highlighted as major assets for CE-MS studies involving native proteins. Thanks to its protein desorption property, improved peak shape proteins during CE at pH. neutral developed in study simple prepare demonstrated high stability pH 7.4, enabling use MS detector. combination these two key elements enabled us successfully convert our method coagulation factor XIIa into ACE-MS approach This extends scope medicinal chemistry projects.
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 389 - 412
Published: Jan. 1, 2025
Language: Английский
Citations
0
Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(2)
Published: Jan. 30, 2025
ABSTRACT Monoclonal antibodies (mAbs) are a key class of biotherapeutic medicines used to treat wide range diseases, such as cancer, infectious autoimmune disorders, cardiovascular and hemophilia. They can be engineered for greater effectiveness specific applications while maintaining their structural elements immune targeting. Traditional immunoglobulin treatments have limited therapeutic uses various adverse effects. That makes mAbs show rapid growth in the pharmaceutical market, with over 250 clinical studies. Although offer higher specificity, they less effective against complex antigens. become essential treating diseases medical options, providing innovative solutions that improve patients' quality life through increasing survival rates, shortening length stay hospitals an improved treatment outcome, reducing side This review outlines mechanisms, applications, advancements mAbs, highlighting transformative role modern medicine potential shape future interventions.
Language: Английский
Citations
0
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2025, Volume and Issue: unknown, P. 189284 - 189284
Published: Feb. 1, 2025
Language: Английский
Citations
0
Methods in cell biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12
Published: May 9, 2025
Antibodies have been widely used globally over the past decade and play an increasingly important role in modern medicine. Notably, significant advancements achieved realm of gynecology, particularly gynecological cancers. This study endeavors to present a thorough overview antibody-related drug clinical studies gynecology registered on ClinicalTrials.gov , focusing basic characteristics trials, geographical distribution, administration routes, indications, targets. The analysis indicates rising prevalence antibody–drug conjugates (ADCs), bispecific antibodies, Fc-fusion proteins. will help develop new ideas for future research antibodies gynecology.
Language: Английский
Citations
0
Advances in protein chemistry and structural biology, Journal Year: 2024, Volume and Issue: unknown, P. 135 - 178
Published: Jan. 1, 2024
Language: Английский
Citations
2