Design of targeted antiviral polypeptides specific to SARS-CoV-2. Challenges and prospects

Russian Chemical Reviews, Journal Year: 2024, Volume and Issue: 93(6), P. RCR5128 - RCR5128

Published: June 1, 2024

The COVID-19 epidemic demanded the rapid development of high-affinity molecules different types aimed at a single target, S-protein SARS-CoV-2. simultaneous and testing such provide unique opportunity to compare features biotechnological platforms for creating therapeutic proteins. This review considers classical antibodies, variable lymphocyte receptors, single-domain artificial scaffolds (DARPins, affibodies, VH), that are compared in terms affinity, neutralizing activity, size compatibility with delivery methods. It can be concluded all used have produced proteins specifically bind coronavirus S-protein. highest affinity targeting virus protein was achieved by developing nanobodies combining several binding modules into multivalent constructs high avidity. Based on results <i>in vivo</i> experiments, it surface antigens SARS-CoV-2 is necessary but not sufficient condition suppression due peculiarities biology this virus. experience gained agents against will useful design effective targeted drugs treatment known new viral infections.<br>The bibliography includes 126 references.

Language: Английский

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Optimization of the Process of Chinese Hamster Ovary (CHO) Cell Fed-Batch Culture to Stabilize Monoclonal Antibody Production and Overall Quality: Effect of pH Control Strategies

Fermentation, Journal Year: 2024, Volume and Issue: 10(7), P. 352 - 352

Published: July 12, 2024

Monoclonal antibodies (mAbs) used in biomedical research and therapeutic applications are primarily produced by Chinese hamster ovary (CHO) cells via fed-batch culture. The growing need for elevated quantities of biologics mandates the continual optimization mAb production process. development an effective process control method is indispensable specified mAbs CHO cells. In this study, effects pH strategy on cell culture to produce antibody (EA5) were first investigated a 3 L bioreactor. results indicate that controlling at 7.2 during stage could higher EA5 titer 6.1 g/L with lower Man5 ratio 2.2% day 14. Based this, optimized was conducted 15 bioreactor verify its effectiveness stability. case, 14, 6.5 achieved productivity 0.46 g/L/day, which 1.07-fold compared Furthermore, regarding product quality, monomer abundance 96.0%, main peak 55.0%, proportion 2.4% maintained addition, different clarification processes evaluated using broth from bioreactors further improve economic performance. Overall, study provides some directions intensification improving quality biopharmaceutical industry.

Language: Английский

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Varianten biotechnologischer Arzneistoffe in der Dermatologie

Deleted Journal, Journal Year: 2024, Volume and Issue: 75(10), P. 775 - 780

Published: Aug. 22, 2024

Biotechnologische Arzneistoffe, sog. Biopharmazeutika, besitzen eine komplexe Struktur, verfügen über physikochemische Besonderheiten und unterliegen speziellen regulatorischen Gesetzmäßigkeiten. Neben rekombinanten monoklonalen Antikörpern, Proteinen Fusionsproteinen ist Vielzahl biotechnologischer Varianten entwickelt worden, von denen insbesondere Antikörperfragmente, Nanobodies, Peptide Antikörper-Wirkstoff-Konjugate Einzug in die praktische Anwendung gefunden haben. Strategien zur Therapie onkologischer Erkrankungen werden v. a. chronisch-entzündliche adressiert, mittlerweile auch der Dermatologie einen großen Stellenwert besitzen. Die Vorteile Biopharmazeutika zunehmend im Rahmen spezieller für topische Applikation erkannt entwickelt. Durch rasante Entwicklung molekularen Medizin zudem laufend neue Targets identifiziert durch verfeinerte Methoden pharmakokinetisch günstige biotechnologische Moleküle kreiert. Es davon auszugehen, dass diese noch weitere hochinnovative Therapie- Diagnostikmöglichkeiten hervorbringen wird.

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The Basics of Large-Scale Commercial Production of Monoclonal Antibodies

Published: Jan. 1, 2024

Language: Английский

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Advancements in peptide-based gas biosensors

Comprehensive analytical chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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