Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(10)
Published: Oct. 1, 2024
Language: Английский
Journal of Cancer Research and Clinical Oncology, Journal Year: 2025, Volume and Issue: 151(2)
Published: Jan. 24, 2025
The cell division cycle associated 4 (CDCA4) plays a crucial role in various biological processes and is implicated the progression of several tumors, however, mechanisms by which it operates bladder cancer remain unclear. Utilizing data from TCGA GEO datasets patients, we analyzed expression CDCA4 its prognostic significance. We then constructed stable overexpression knockdown lines to investigate effects on proliferation, migration, invasion vitro, employing CCK-8, colony formation, transwell, wound healing assays. Additionally, validated potential downstream pathways through analysis western blot Our study found that elevated cells correlates with poor prognosis patients. Inhibition reduces cells, as well inhibit epithelial-mesenchymal transition (EMT) process. Conversely, promoting enhances malignancy cells. Investigation into mechanism revealed promotes activating JAK/STAT signaling pathway, JAK inhibitor AG490 can reverse CDCA4. findings suggest positively regulating indicating may serve novel molecular target for treatment.
Language: Английский
Citations
0
Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)
Published: Jan. 1, 2025
Abstract This study was to analyze the effect of triptolide (TPL) on proliferation, apoptosis, and relationship between TPL Janus kinase/signal transducer activator transcription signaling pathway in hepatoma cells. HepG2 cell line selected as experimental object divided into control, low-dose TPL, medium-dose high-dose group. The control group did not receive any drug treatment, while low, medium, groups were treated with at concentrations 0.02, 0.05, 0.10 μM, respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, flow cytometry, western blot used detach mechanism. proliferation inhibition rate each dose lower than that group, increased increase ( P < 0.05). apoptosis higher expression phosphorylated kinase 1 (p-JAK1) signal 3 (p-STAT3) protein cells p-JAK1 p-STAT3 decreased 10 ng/mL transforming growth factor-beta + reduced activity B-cell lymphoma/leukemia-2-associated X (Bax) cysteine aspartic acid protease (Caspase)-3/9 raised lymphoma/leukemia-2 (Bcl-2) With dose, Bax Caspase-3/9 increased, Bcl-2 As an anti-liver cancer agent, inhibits hepatocellular carcinoma promotes apoptosis. mechanism may involve inhibiting 1/signal activation apoptosis-related pathways.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 17, 2025
Metastasis is a hallmark of advanced cancer, and the liver common site for secondary metastasis many tumor cells, including colorectal, pancreatic, gastric, prostate cancers. Macrophages in microenvironment (TME) promote cell through various mechanisms, angiogenesis immunosuppression, play unique role development metastasis. are affected by variety factors. Under conditions hypoxia increased acidity TME, more factors now found to polarization macrophages M2 type, exosomes amino acids. M2-type secretion such as VEGF, IL-1β, TGF-β1. subjected multiple regulatory mechanisms. They also interact with cells within co-regulate certain conditions, creation an immunosuppressive microenvironment. This interaction promotes metastasis, drug resistance, immune escape. Based on advent single-cell sequencing technology, further insights into macrophage subpopulations may help exploring new therapeutic targets future. In this paper, we will focus how affect well other each other, investigate mechanisms involved their potential targets.
Language: Английский
Citations
0
Growth Factors, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11
Published: April 3, 2025
DEAD-box RNA helicase (DDX) is linked to the invasion, drug resistance, proliferation, and epithelial-mesenchymal transition of tumour cells. This study examined potential mechanisms DDX49 in breast cancer. The expression cancer tissues cells was evaluated. effects on migration apoptosis were proteins associated with JAK/STAT pathway examined. A xenograft model established. elevated cell lines. shDDX49 suppressed ability proliferate, invade, migrate, but promoted apoptosis. Conversely, overexpression exerted an opposite effect. activation JAK-STAT signalling inhibited by shDDX49. efficiently inhibits growth mice may hinder spread inhibiting pathway.
Language: Английский
Citations
0
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: April 8, 2025
Abstract Baicalin has been widely used for its anti‐inflammatory pharmacological properties, yet effects on bacterial intestinal inflammation and the mechanisms remain unclear. This study revealed that baicalin alleviates through regulating macrophage polarization increasing Lactobacillus amylovorus abundance in colon. Specifically, transcriptomic analysis showed restored Escherichia coli ‐induced genes expression changes including T helper cell 17 differentiation‐related genes, related TLR/IRF/STAT signaling pathway. Subsequent microbial non‐targeted metabolomic these may be to enhancement of upregulation metabolites chrysin, lactic acid, indoles. Furthermore, whole‐genome sequencing provided insights into functional potential metabolic annotations. supplementation mice similarly inhibited M1 TLR4/IRF/STAT Additionally, baicalin, , or chrysin alone could regulate polarization, highlighting their independent potential. Notably, this pathway synthesis chrysin. These findings provide new therapeutic preventing treating inflammation, offering key targets future interventions.
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: April 25, 2025
Although macrophages and lipid metabolism significantly influence the progression of various cancers, their precise roles in pancreatic cancer (PC) remain unclear. This study focuses on identifying validating biomarkers associated with macrophage-related genes (MRGs) metabolism-related (LMRGs), providing new targets strategies for therapeutic intervention. research utilized datasets from TCGA-PAAD, GSE62452, GSE57495. Candidate were identified by overlapping differentially expressed MRGs WGCNA LMRGs. Regression analyses performed to pinpoint potential construct a risk model, which underwent evaluation. A nomogram was subsequently developed validated. Additional analyses, including functional enrichment, somatic mutation profiling, immune landscape assessment, RT-qPCR, investigate underlying biological mechanisms PC. The ADH1A, ACACB, CD36, CERS4, PDE3B, ALOX5, CRAT as RT-qPCR results revealed reduced expression tumor samples compared adjacent tissues, whereas ALOX5 elevated samples. model utilizing these classified PC patients into high- low-risk cohorts, high-risk showing lower survival probabilities. Subsequently, score N stage independent prognostic factors, leading development nomogram. Notably, both cohorts showed significant enrichment "cell cycle" pathway. Furthermore, TP53 mutations prevalent (76%) (50%) cohorts. Correlation analysis indicated that PVRL2 (an immunosuppressive factor), CD276 immunoactivator), CCL20 (a chemotactic factor) had highest positive correlation score. In this study, PC, levels validated clinical These findings offered theoretical foundation developing targeted treatments
Language: Английский
Citations
0
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: May 2, 2025
Acute kidney injury (AKI) is a life-threatening condition characterized by rapid decline in the renal function, primarily caused oxidative stress, inflammation, and ferroptosis. Herein, we present selenium-doped carbon dots (Zt-SeCDs) that integrate antioxidant activity with controlled release of Zileuton, 5-lipoxygenase (ALOX5) inhibitor, under high reactive oxygen species (ROS) conditions. This nanoplatform can efficiently deliver leveraging its inherent properties to achieve targeted prevention treatment AKI. In vitro studies have confirmed Zt-SeCDs eliminate excessive ROS, prevent ferroptotic cell death, modulate inflammatory responses reducing expression key pro-inflammatory cytokines. Additionally, regulate ferroptosis through suppression ALOX5 upregulation glutathione peroxidase 4 (GPX4) expression. The significantly improves promotes regeneration damaged tissue, alleviates processes, death. Moreover, monitoring serum indicators observing pathological changes further potential preventing Notably, activate AMPK/FoxO1 signaling pathway, enhancing endogenous defenses protect tissue from damage. promising not only holds significant promise for AKI, but also aims facilitate clinical application.
Language: Английский
Citations
0
Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 16
Published: May 14, 2025
Purpose M2 phenotype tumor-associated macrophages (TAMs) can promote tumor growth, invasion, chemotherapy resistance and so on, leading to malignant progression. The aim of this study was identify novel prognostic profiles in glioblastoma (GBM) by integrating single-cell RNA sequencing (scRNA-seq) with bulk RNA-seq. Methods We identified M2-associated genes intersecting TAM marker derived from scRNA-seq macrophage module WGCNA RNA-seq data. Prognostic TAM-related were determined using univariate Cox LASSO regression analyses. In the following steps, characteristics, risk groups, external validation constructed validated. immune landscape patients GBM examined evaluating cells, functions, evasion scores, checkpoint genes. Results Analysis bulk-seq data revealed 107 linked TAMs. Using regression, 16 as for GBM, creation a signature survival prediction. Conclusion Our findings reveal enhance understanding molecular mechanisms associated
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3961 - 3961
Published: April 2, 2024
The persisting presence of opportunistic pathogens like Pseudomonas aeruginosa poses a significant threat to many immunocompromised cancer patients with pulmonary infections. This review highlights the complexity interactions in host's defensive eicosanoid signaling network and its hijacking by pathogenic bacteria their own advantage. Human lipoxygenases (ALOXs) mouse counterparts are integral elements innate immune system, mostly operating pro-inflammatory mode. Taking into account indispensable role inflammation carcinogenesis, have counteracting roles this process. In addition describing structure-function review, we discuss such critical processes as cell signaling, metastases, death cells through ferroptosis, well ALOXs carcinogenesis promoted Finally, perspectives novel oncotherapeutic approaches harness lipoxygenase tumors.
Language: Английский
Citations
3
Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)
Published: Aug. 26, 2024
Pancreatic cancer (PC) is one of the deadliest cancers worldwide with low survival rates and poor outcomes. The treatment landscape for PC fraught obstacles, including drug resistance, lack effective targeted therapies immunosuppressive tumor microenvironment (TME). resistance to existing immunotherapies highlights need innovative approaches, TME emerging as a promising therapeutic target. recent advancements in understanding role macrophages, this context highlight their significant impact on development progression. There are two important types macrophages: M1 M2, which play critical roles TME. Therapeutics strategies including, depletion tumor-associated macrophages (TAMs), reprogramming TAMs promote anti-tumor activity, targeting macrophage recruitment can lead Targeting macrophage-related pathways may offer novel modulating immune responses, inhibiting angiogenesis, overcoming chemotherapy treatment.
Language: Английский
Citations
3