Pathological mechanisms and related markers of steroid-induced osteonecrosis of the femoral head DOI Creative Commons
Longyu Li,

Shangkun Zhao,

Zikuan Leng

et al.

Annals of Medicine, Journal Year: 2024, Volume and Issue: 56(1)

Published: Nov. 12, 2024

Osteonecrosis of the femoral head (ONFH) is a refractory orthopedic disease with high disability rate. Long-term administration steroids most common pathogenic factor for non-traumatic ONFH. Early diagnosis steroid-induced osteonecrosis (SONFH) difficult and mainly depends on imaging.

Language: Английский

CD4 T cell-secreted IFN-γ in Sjögren's syndrome induces salivary gland epithelial cell ferroptosis DOI Creative Commons

Jiannan Zhou,

Janak L. Pathak,

Tingting Cao

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(4), P. 167121 - 167121

Published: March 11, 2024

Language: Английский

Citations

10
Integrated bioinformatics analysis and experimental validation of exosome-related gene signature in steroid-induced osteonecrosis of the femoral head DOI Creative Commons
Renqun Mao, Wen Bi, Meng-Yue Yang

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 9, 2025

Steroid-induced osteonecrosis of the femoral head (SIONFH) is a universal hip articular disease and very hard to perceive at an early stage. The understanding pathogenesis SIONFH still limited, identification efficient diagnostic biomarkers insufficient. This research aims recognize validate latent exosome-related molecular signature in diagnosis by employing bioinformatics investigate mechanisms SIONFH. GSE123568 GSE74089 datasets were employed conduct differentially expressed genes (DEGs) analysis, dataset was subjected perform weighted co-expression network analysis (WGCNA). (ERGs) retrieved from GeneCards database. We identified (DEERGs) between healthy controls (HC) patients, consensus clustering then implemented group patients. CIBERSORT calculate immune cell infiltration. Gene Set Variation Analysis (GSVA), Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) conducted enriched pathways. In addition, machine-learning algorithms applied refine DEERGs. Ultimately, we verified significance expression hub using performing quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. study twenty DEERGs peripheral serum cartilage samples patients HC. Two subtypes related ERGs identified, distinctions pathways infiltration patterns compared. SIONFH's high-risk subpopulation exhibited immune-related high infiltration, such as M0 macrophages, resting mast cells, neutrophils. Three determined LCP1, PNP, UBE2V1, ZFP36 four (ERHGs). Compared HC samples, these ERHGs showed excellent efficiency (overall AUC for range 0.923 0.970 GSE123568) samples. expressions validated finally detected with accordant RT-qPCR. Twenty potential involved through might become candidate therapeutic targets because they have intimate relationship exosomes. These findings shed light on acquaintance contribute prognosis

Language: Английский

Citations

1
MiR-370-3p regulate TLR4/SLC7A11/GPX4 to alleviate the progression of glucocorticoids-induced osteonecrosis of the femoral head by promoting osteogenesis and suppressing ferroptosis DOI Creative Commons

Rongtai Zuo,

Bojun Cao, Lingchi Kong

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

1
Advances in Ferroptosis and Glucocorticoid-Induced Osteonecrosis of Femoral Head and Discussion of Related Ferroptosis Genes in Bone Metabolism or Oxidative Stress DOI

绍庚 王

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(01), P. 1717 - 1730

Published: Jan. 1, 2025

Language: Английский

Citations

0
Knockdown of lncRNA XR_877193.1 suppresses ferroptosis and promotes osteogenic differentiation via the PI3K/AKT signaling pathway in SONFH DOI Creative Commons
Huixia Yang, Ning Ding,

Qing Shi

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Ferroptosis is a novel form of regulated cell death characterized by the iron-dependent accumulation lipid peroxides. Recent research has suggested that ferroptosis in osteoblasts contributes to steroid-induced osteonecrosis femoral head (SONFH). However, relationship between and SONFH remains unclear. In this study, vitro experiments show dexamethasone (Dex) treatment reduces expressions key regulators, SLC7A11 GPX4, MC3T3-E1 cells. This reduction leads decrease intracellular glutathione (GSH) levels, accompanied elevated levels total iron, malondialdehyde (MDA), reactive oxygen species (ROS). Importantly, inhibitor ferrostatin-1 (Fer-1) effectively reverses Dex-induced Furthermore, RNA-seq analysis reveals long noncoding RNA (lncRNA) XR_877193.1is significantly upregulated Dex-treated Functional studies demonstrate knockdown lncRNA XR_877193.1 promotes osteogenic differentiation inhibiting cells, whereas its overexpression exacerbates via ferroptosis. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment differentially expressed enriched ferroptosis-related pathways, including PI3K/AKT signaling pathway. Moreover, inhibitors reverse cells inhibited knockdown. Collectively, our findings indicate exerts anti-ferroptosis effects stimulating pathway, suggesting promising therapeutic strategy for attenuating SONFH.

Language: Английский

Citations

0
Identification and validation of ferroptosis-related gene SLC2A1 as a novel prognostic biomarker in AKI DOI Creative Commons
Huaying Wang, Yuanyuan Li, Xinran Liu

et al.

Aging, Journal Year: 2024, Volume and Issue: 16(6), P. 5634 - 5650

Published: March 20, 2024

Background: Emerging evidence reveals the key role of ferroptosis in pathophysiological process acute kidney injury (AKI). Our study aimed to investigate potential ferroptosis-related gene AKI through bioinformatics and experimental validation. Methods: The single-cell sequencing dataset was retrieved from GEO database genes were extracted GENECARD website. differentially expressed selected. Functional enrichment analysis performed. Machine learning algorithms used identify associated with AKI. A multi-factor Cox regression construct a risk score model. accuracy model validated using receiver operating characteristic (ROC) curve analysis. We extensively explored immune landscape CIBERSORT tool. Finally, expressions DEGs vivo vitro by Western blot, ICH transfection experiments. Results: Three hub (BAP1, MDM4, SLC2A1) identified constructing drug regulatory network subsequent screening experimentally determined interactions. mode showed low-risk group had significantly better prognosis compared high-risk group. independently overall survival. ROC that good predictive ability. Additionally, infiltration suggest may influence cell recruitment Validation experiments revealed SLC2A1 functions regulating ferroptosis. Conclusions: In summary, our not only identifies as diagnostic biomarker for AKI, but also sheds light on it progression, providing novel insights clinical diagnosis treatment

Language: Английский

Citations

2
Pathological mechanisms and related markers of steroid-induced osteonecrosis of the femoral head DOI Creative Commons
Longyu Li,

Shangkun Zhao,

Zikuan Leng

et al.

Annals of Medicine, Journal Year: 2024, Volume and Issue: 56(1)

Published: Nov. 12, 2024

Osteonecrosis of the femoral head (ONFH) is a refractory orthopedic disease with high disability rate. Long-term administration steroids most common pathogenic factor for non-traumatic ONFH. Early diagnosis steroid-induced osteonecrosis (SONFH) difficult and mainly depends on imaging.

Language: Английский

Citations

1