Astragalus mongholicus and Hedyotis diffusa Willd Inhibit Cell Proliferation by Attenuating the miR-582–3p-p27 Signaling Pathway in LUAD DOI Creative Commons

Haipeng Sun,

Ling Xu, Siyuan Liu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Abstract Purpose Previous studies conducted by the same group of researchers found that Traditional Chinese Medicine Astragalus mongholicus Bunge and Hedyotis diffusa Willd (A-H pair) significantly suppressed cell proliferation lung adenocarcinoma (LUAD). MicroRNAs are considered promising candidates for cancer diagnosis treatment. This study focused on miR-582-3p as primary subject investigation to explore mechanism which A-H inhibits through miR-582-3p. Methods The overexpressing silencing models were established using lentiviral transfection technology. CCK-8 assay (24 h, 48 72 h) clone formation (1 w) employed detect A549 cells. Moreover, flow cytometry analysis was performed cycle. Western blotting (WB) a luciferase reporter also used measure expression cycle-related proteins verify direct interaction between p27, respectively. LV-miR-582-3p inhibitor + shRNA-p27 stable cells constructed in manner repeat above-mentioned procedure. assess effects various concentrations A-H-containing serum prepared intervene mimic Subsequently, procedure repeated, described earlier. Results indicated p27. Furthermore, enhance regulating proteins, specifically It observed inhibited miR-582-3p-p27 signaling pathway. Conclusion findings revealed underlying molecular mechanisms development prognosis LUAD In addition, These may provide new understanding use medicine treating cancer.

Language: Английский

Long noncoding RNA SNHG4 promotes glioma progression via regulating miR-367-3p/MYO1B axis in zebrafish xenografts DOI Creative Commons
Yueqing Zhang, Yongjin Wang, Yang� Yang

et al.

Human Cell, Journal Year: 2025, Volume and Issue: 38(2)

Published: Feb. 14, 2025

Abstract Glioma is one of the most malignancy and prevalent tumor in human central nervous system, which associated with severe morbidity high mortality. Numerous studies have explained clear correlation between abnormal expression lncRNA progression Glioma. LncRNA small nucleolar RNA host gene 4 (SNHG4) been proved to play oncogenesis roles various tumors, however, underlying mechanism remains be explored deeply. In this study, by analysis public database, we found that SNHG4 was upregulated multiple cancer tissues, including glioma. Subsequently, functional were investigated, knockdown remarkedly inhibited cell proliferation, migration. While, overexpression enhanced these functions glioma cells vitro. Meanwhile, as vivo tool, zebrafish xenograft model used verify cells. Mechanically, identified or MYO1B could bind miR-367-3p luciferase reporter assays. Furthermore, rescue experiments showed inhibition partially effects Our study reveals promotes migration via regulating miR-367-3p/MYO1B axis.

Language: Английский

Citations

1
Epigenetic modifications in bladder cancer: crosstalk between DNA methylation and miRNAs DOI Creative Commons
Wei Li,

Peiyue Luo,

Qi Chen

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 5, 2025

Bladder cancer (BC) is a malignant tumor characterized by high incidence of urinary system diseases. The complex pathogenesis BC has long been focal point in medical research. With the robust development epigenetics, crucial role epigenetic modifications occurrence and progression elucidated. These not only affect gene expression but also impact critical biological behaviors cells, including proliferation, differentiation, apoptosis, invasion, metastasis. Notably, DNA methylation, an important regulatory mechanism, often manifests as global hypomethylation or hypermethylation specific promoter regions BC. Alterations this methylation pattern can lead to increased genomic instability, which profoundly influences proto-oncogenes suppressor genes. MiRNAs, noncoding small RNAs, participate various processes regulating target Consequently, work aims explore interaction mechanisms between miRNAs Research demonstrated that directly miRNA genes indirectly affects maturation functionality modulating status regions. Simultaneously, regulate levels targeting key enzymes such methyltransferases (DNMTs), thereby establishing feedback network. A deeper understanding crosstalk will contribute elucidating complexity dynamics disease, may provide new molecular targets strategies for early diagnosis, treatment, prognostic evaluation

Language: Английский

Citations

0
Recent animal models of bladder cancer and their application in drug discovery: an update of the literature DOI
Inês Aires, B. Parada, Rita Ferreira

et al.

Expert Opinion on Drug Discovery, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Introduction Bladder cancer presents a significant health problem worldwide, with environmental and genetic factors contributing to its incidence. Histologically, it can be classified as carcinoma in situ, non-muscle invasive muscle-invasive carcinoma, each one distinct alterations impacting prognosis response therapy. While traditional transurethral resection is commonly performed situ often fails prevent recurrence or progression more aggressive phenotypes, leading the frequent need for additional treatment such intravesical chemotherapy immunotherapy. Despite advances made recent years, options bladder are still lacking due complex nature of this disease. So, animal models may hold potential addressing these limitations, because they not only allow study disease but also evaluation therapies investigation drug repositioning.

Language: Английский

Citations

0
Astragalus mongholicus and Hedyotis diffusa willd inhibit cell proliferation by attenuating the miR-582-3p-p27 signaling pathway in LUAD DOI Creative Commons

Haipeng Sun,

Ling Xu, Siyuan Liu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Language: Английский

Citations

0
Non-coding RNAs in bladder cancer, a bridge between gut microbiota and host? DOI Creative Commons
Jun Zou, Baisheng Xu,

Peiyue Luo

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 19, 2024

In recent years, the role of gut microbiota (GM) in bladder cancer has attracted significant attention. Research indicates that GM not only contributes to carcinogenesis but also influences efficacy adjuvant therapies for cancer. Despite this, interventions targeting have been widely employed prevention and treatment cancer, mainly due incomplete understanding complex interactions between host flora. Simultaneously, aberrantly expressed non-coding RNAs (ncRNAs) frequently associated with playing crucial roles processes such as cell proliferation, invasion, drug resistance. It is known regulation GM-mediated pathophysiological partly regulated through epigenetic pathways. At same time, ncRNAs are increasingly regarded signaling molecules involved regulation. Accordingly, this review analyzes closely related context occurrence treatment, summarizes their interaction cancer-related phenotypes. The aim delineate a regulatory network provide new perspective study

Language: Английский

Citations

0
Astragalus mongholicus and Hedyotis diffusa Willd Inhibit Cell Proliferation by Attenuating the miR-582–3p-p27 Signaling Pathway in LUAD DOI Creative Commons

Haipeng Sun,

Ling Xu, Siyuan Liu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Abstract Purpose Previous studies conducted by the same group of researchers found that Traditional Chinese Medicine Astragalus mongholicus Bunge and Hedyotis diffusa Willd (A-H pair) significantly suppressed cell proliferation lung adenocarcinoma (LUAD). MicroRNAs are considered promising candidates for cancer diagnosis treatment. This study focused on miR-582-3p as primary subject investigation to explore mechanism which A-H inhibits through miR-582-3p. Methods The overexpressing silencing models were established using lentiviral transfection technology. CCK-8 assay (24 h, 48 72 h) clone formation (1 w) employed detect A549 cells. Moreover, flow cytometry analysis was performed cycle. Western blotting (WB) a luciferase reporter also used measure expression cycle-related proteins verify direct interaction between p27, respectively. LV-miR-582-3p inhibitor + shRNA-p27 stable cells constructed in manner repeat above-mentioned procedure. assess effects various concentrations A-H-containing serum prepared intervene mimic Subsequently, procedure repeated, described earlier. Results indicated p27. Furthermore, enhance regulating proteins, specifically It observed inhibited miR-582-3p-p27 signaling pathway. Conclusion findings revealed underlying molecular mechanisms development prognosis LUAD In addition, These may provide new understanding use medicine treating cancer.

Language: Английский

Citations

0