lncRNA FGD5-AS1 is required for gastric cancer proliferation by inhibiting cell senescence and ROS production via stabilizing YBX1

Journal of Experimental & Clinical Cancer Research, Journal Year: 2024, Volume and Issue: 43(1)

Published: July 5, 2024

The vast majority of lncRNAs have low expression abundance, which greatly limits their functional range and impact. As a high abundance lncRNA, FGD5-AS1's non-ceRNA biological function in cancer is unclear.

Language: Английский

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A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(5)

Published: May 28, 2024

Abstract Transforming growth factor beta (TGFβ) signaling plays a critical role in tumorigenesis and metastasis. However, little is known about the biological function of TGFbeta-induced lncRNA cancer. In this study, we discovered novel TG Fbeta- i nduced l nc R NA, termed TGILR , whose cancer remains unknown to date. expression was directly activated by canonical TGFbeta/SMAD3 axis, activation highly conserved Clinical analysis showed that overexpression significant correlation with lymph node metastasis poor survival an independent prognostic gastric (GC). Depletion caused obvious inhibitory effect on GC cell proliferation, invasion, epithelial-mesenchymal transition (EMT) vitro vivo. More importantly, demonstrated TGFbeta overactivated due cancer-associated fibroblast (CAF) infiltration. Mechanistically, increased level CAF-secreted activates signaling, leading cells. Meanwhile, inhibited microRNA biogenesis miR-1306 miR-33a interacting TARBP2 reducing its protein stability, thereby promoting progression via TCF4-mediated EMT signaling. conclusion, CAF infiltration drives through activating TGFbeta/TGILR axis. Targeted blocking CAF-derived should be promising anticancer strategy GC.

Language: Английский

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Single-cell RNA sequencing to map tumor heterogeneity in gastric carcinogenesis paving roads to individualized therapy

Cancer Immunology Immunotherapy, Journal Year: 2024, Volume and Issue: 73(11)

Published: Sept. 13, 2024

Gastric cancer (GC) is a highly heterogeneous disease with complex tumor microenvironment (TME) that encompasses multiple cell types including cells, immune stromal and so on. Cancer-associated cells could remodel the TME influence progression of GC therapeutic response. Single-cell RNA sequencing (scRNA-seq), as an emerging technology, has provided unprecedented insights into complicated biological composition characteristics at molecular, cellular, immunological resolutions, offering new idea for studies. In this review, we discuss novel findings from scRNA-seq datasets revealing origin evolution GC, powerful tool investigating transcriptional dynamics intratumor heterogeneity (ITH) in GC. Meanwhile, demonstrate vital within TME, T B macrophages, play important role progression. Additionally, also overview how facilitates our understanding about effects on individualized therapy patients. Spatial transcriptomes (ST) have been designed to determine spatial distribution capture local intercellular communication networks, enabling further relationship between background particular its functions. summary, other single-cell technologies provide valuable perspective molecular pathological hold promise advancing basic research clinical practice

Language: Английский

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Tumor cell-derived EMP1 is essential for cancer-associated fibroblast infiltration in tumor microenvironment of triple-negative breast cancer

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 27, 2025

Abstract The role of epithelial membrane protein 1 (EMP1) in tumor microenvironment (TME) remodeling has not yet been elucidated. In addition, the biological function EMP1 triple-negative breast cancer (TNBC) is largely unclear. this study, we examined infiltration landscape cell types TME cancer, and found that expression was positively correlated with stromal microenvironmental scores. Infiltration analysis immunohistochemical (IHC) staining serial sections confirmed critical cancer-associated fibroblast (CAF) infiltration. Cell co-culture assays, xenograft experiments, loss-of-function, gain-of-function, RNA sequencing studies, rescue assays were performed to confirm CAF vitro vivo. These findings revealed depletion TNBC cells resulted considerable inhibition vivo vitro. Mechanistically, knockdown induced a substantial decrease IL6 secretion from through NF-κB signaling pathway, hindering proliferation subsequently inhibiting progression metastasis. cumulative results indicate functions as an oncogene by mediating communication CAFs. Targeted suppressing promising strategy for treatment.

Language: Английский

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The Dynamic Interplay between Melanoma Cells and CAFs: Implications Drug Resistance and Immune Evasion and Possible Therapeutics

Experimental Cell Research, Journal Year: 2025, Volume and Issue: unknown, P. 114581 - 114581

Published: April 1, 2025

Language: Английский

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MIR194-2HG, a miRNA host gene activated by HNF4A, inhibits gastric cancer by regulating microRNA biogenesis

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Oct. 18, 2024

MicroRNA host gene (MIRHG) lncRNA is a particular subclass that can perform both typical and atypical functions. The biological function of MIRHG MIR194-2HG in cancer poorly understood.

Language: Английский

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Single-cell RNA Sequencing Demonstrates the Heterogeneity of Different Molecular Subtypes in Gastric Cancer

Biochemical Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Language: Английский

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Receptor accessory protein 6, a novel ferroptosis suppressor, drives oral squamous cell carcinoma by maintaining endoplasmic reticulum hemostasis

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137565 - 137565

Published: Nov. 1, 2024

Language: Английский

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