Bioinformatics based exploration of the anti-NAFLD mechanism of Wang’s empirical formula via TLR4/NF-κB/COX2 pathway DOI Creative Commons
Suhong Chen,

Chuanjie Zhou,

Jia-Hui Huang

et al.

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Dec. 27, 2024

Abstract Background Nonalcoholic fatty liver disease (NAFLD) has developed as a leading public wellness challenge result of changes in dietary patterns. Unfortunately, there is still lack effective pharmacotherapy methods for NAFLD. Wang’s empirical formula (WSF) demonstrated considerable clinical efficacy treating metabolic disorders years. Nevertheless, the protective effect WSF against NAFLD and its underlying mechanism remains poorly understood. Methods The model was established using 17-week high-sucrose high-fat (HSHF) diet with 32 ICR mice. In assessing therapeutic on NAFLD, we detected body weight, viscera biomarkers glycolipid metabolism serum liver, transaminase levels histopathology H&E Oil Red O staining after oral administration. chemical components were extensively identified gathered utilizing HPLC-Q-TOF/MS system, database mining from HMDB, MassBank, TCMSP databases, alongside literature searches CNKI, Wanfang VIP databases. forecast network pharmacology approach then utilized to investigate probable mechanisms by which improves based performance prospective target identification pathway enrichment analysis. Besides, molecular docking also conducted verification combination activities between active core proteins related final, validation experiments obtained pathways through ELISA, immunohistochemistry (IHC), western blot (WB) Results Pharmacodynamic outcomes indicated that intervention effectively mitigated obesity, fat accumulation organs, lipid disorders, abnormal pathology injury mice ( P < 0.05, 0.01). A total 72 existent ingredients acquired database, 254 common targets (11.6% targets) identified. Network revealed presses via modulating TNF, IL6, AKT1, IL1B, PTGS2 (COX2), other targets, primarily inflammatory signaling pathways, confirmed docking. Molecular biology further conformed could decrease factors like IL-1β, IL-6 TNF-α 0.01) expression TLR4, NF-κB COX-2 liver. Conclusion treatment protects inflammation HSHF diet-induced mice, might be suppressing TLR4/NF-κB/COX-2 reduce release cytokines Graphical abstract

Language: Английский

LC-HRMS profiling of Dendrobium huoshanense aqueous extract and its therapeutic effects on nonalcoholic fatty liver disease in mice through the TLR2-NF-κB and AMPK-SREBP1-SIRT1 signaling pathways DOI
Qiyan Lin, Huang Ke, Xiyu Ge

et al.

Journal of Chromatography B, Journal Year: 2025, Volume and Issue: unknown, P. 124563 - 124563

Published: March 1, 2025

Language: Английский

Citations

0
Recent advances in the role of polysaccharides in liver diseases: a review DOI Creative Commons
Junfeng Wang, Hanxiang Wang,

Xiawen Yang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 27, 2025

Liver diseases are a serious health problem worldwide, especially with sustained increase in the burden of it every year. However, drugs commonly used patients have limited efficacy and adverse reactions associated long-term use. Therefore, is urgent to find effective safe alternatives. Polysaccharides currently considered promising alternatives traditional because their extensive activity low toxicity. This review investigated studies on hepatoprotective polysaccharides over past 6 years, detailing effects, potential mechanisms, drug carrier applications. These findings suggest that prominent preventive therapeutic effects various liver such as drug-induced injury, alcoholic disease, hepatitis B, non-alcoholic fatty fibrosis, hepatocellular carcinoma. Its mechanism includes multiple aspects metabolic regulation, reduction oxidative stress inflammation, regulation gut microbiota. Furthermore, owing good physicochemical properties, been applied delivery systems for chemotherapy small molecule drugs. further research essential bioavailability, structure-activity relationship, more clinical evidence polysaccharides. Continued exploration will provide tremendous treatment diseases.

Language: Английский

Citations

0
Calpain-1 is required for abnormality of liver function and metabolism in apolipoprotein E knockout mouse evaluated noninvasively by small animal MRI and PET-CT DOI

Madiha Awad,

Wenjiao Gu,

Shuangyi Li

et al.

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Journal Year: 2025, Volume and Issue: unknown, P. 159616 - 159616

Published: April 1, 2025

Language: Английский

Citations

0
Bioinformatics based exploration of the anti-NAFLD mechanism of Wang’s empirical formula via TLR4/NF-κB/COX2 pathway DOI Creative Commons
Suhong Chen,

Chuanjie Zhou,

Jia-Hui Huang

et al.

Molecular Medicine, Journal Year: 2024, Volume and Issue: 30(1)

Published: Dec. 27, 2024

Abstract Background Nonalcoholic fatty liver disease (NAFLD) has developed as a leading public wellness challenge result of changes in dietary patterns. Unfortunately, there is still lack effective pharmacotherapy methods for NAFLD. Wang’s empirical formula (WSF) demonstrated considerable clinical efficacy treating metabolic disorders years. Nevertheless, the protective effect WSF against NAFLD and its underlying mechanism remains poorly understood. Methods The model was established using 17-week high-sucrose high-fat (HSHF) diet with 32 ICR mice. In assessing therapeutic on NAFLD, we detected body weight, viscera biomarkers glycolipid metabolism serum liver, transaminase levels histopathology H&E Oil Red O staining after oral administration. chemical components were extensively identified gathered utilizing HPLC-Q-TOF/MS system, database mining from HMDB, MassBank, TCMSP databases, alongside literature searches CNKI, Wanfang VIP databases. forecast network pharmacology approach then utilized to investigate probable mechanisms by which improves based performance prospective target identification pathway enrichment analysis. Besides, molecular docking also conducted verification combination activities between active core proteins related final, validation experiments obtained pathways through ELISA, immunohistochemistry (IHC), western blot (WB) Results Pharmacodynamic outcomes indicated that intervention effectively mitigated obesity, fat accumulation organs, lipid disorders, abnormal pathology injury mice ( P < 0.05, 0.01). A total 72 existent ingredients acquired database, 254 common targets (11.6% targets) identified. Network revealed presses via modulating TNF, IL6, AKT1, IL1B, PTGS2 (COX2), other targets, primarily inflammatory signaling pathways, confirmed docking. Molecular biology further conformed could decrease factors like IL-1β, IL-6 TNF-α 0.01) expression TLR4, NF-κB COX-2 liver. Conclusion treatment protects inflammation HSHF diet-induced mice, might be suppressing TLR4/NF-κB/COX-2 reduce release cytokines Graphical abstract

Language: Английский

Citations

0