Bioinformatics Identification of Lactate‐Associated Genes in Hepatocellular Carcinoma: G6PD's Role in Immune Modulation

Cancer Medicine, Journal Year: 2025, Volume and Issue: 14(6)

Published: March 1, 2025

ABSTRACT Background Hepatocellular carcinoma (HCC) is a major global health issue, with poor prognosis often associated dysregulated metabolic pathways, especially lactate metabolism. This study explored the prognostic significance of lactate‐associated genes in HCC and their potential as therapeutic targets. Methods We analyzed RNA‐seq clinical data from 374 patients The Cancer Genome Atlas (TCGA) database. Using Cox regression, LASSO analysis, Kaplan–Meier survival curves, we identified key patient outcomes. Functional validations, including Western blot, flow cytometry, molecular docking studies, were performed to confirm biological impact these genes. Results G6PD, IK, CALML5 significant markers for HCC. A model was developed that effectively stratified into risk groups, which correlated survival. G6PD's role immune modulation its drug target validated through biochemical assays computational analyses. HepG2 cells confirmed alterations G6PD expression affect T cell activity, knockdown enhancing IFN‐γ production overexpression inhibiting it, demonstrating evasion. Conclusions establishes metabolism genes, particularly validation immunomodulatory effects further supports strategies aimed at surveillance treatment outcomes

Language: Английский

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Bioinformatics Identification of Lactate-Associated Genes in Hepatocellular Carcinoma: G6PD’s Role in Immune Modulation

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Background Hepatocellular carcinoma (HCC) is a major global health issue, with poor prognosis often associated dysregulated metabolic pathways, especially lactate metabolism. This study explored the prognostic significance of lactate-associated genes in HCC and their potential as therapeutic targets. Methods We analyzed RNA-seq clinical data from 374 patients The Cancer Genome Atlas (TCGA) database. Using Cox regression, LASSO analysis, Kaplan-Meier survival curves, we identified key patient outcomes. Functional validations, including Western blot, flow cytometry, molecular docking studies, were performed to confirm biological impact these genes. Results G6PD, IK, CALML5 significant markers for HCC. A model was developed that effectively stratified into risk groups, which correlated survival. G6PD’s role immune modulation its drug target validated through biochemical assays computational analyses. HepG2 cells confirmed alterations G6PD expression affect T cell activity, knockdown enhancing IFN-γ production overexpression inhibiting it, demonstrating evasion. Conclusions establishes metabolism genes, particularly validation immunomodulatory effects further supports strategies aimed at surveillance treatment outcomes

Language: Английский

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SiO2 particles induce pulmonary fibrosis by modulating NLRP3 through the ROS/Keap1/Nrf2 signaling pathway in rats

Food and Chemical Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 115520 - 115520

Published: May 1, 2025

Language: Английский

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Exosomal miRNA reprogramming in pyroptotic macrophage drives silica-induced fibroblast-to-myofibroblast transition and pulmonary fibrosis

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 483, P. 136629 - 136629

Published: Nov. 22, 2024

Language: Английский

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