Voglibose Attenuates Amyloid Beta–Induced Memory Deficits in a Rodent Model: A Potential Alzheimer’s Therapy via Wnt Signaling Modulation DOI Creative Commons
Suman Manandhar, Prasada Chowdari Gurram, Anusha Govindula

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 17, 2025

Disruption of the Wnt signaling pathway (WSP), a highly conserved essential for growth and organ development, has been proven to play role in pathogenesis Alzheimer's disease (AD). This study focused on repurposing FDA-approved drug, Voglibose target DKK1-LRP6 site with goal upregulating WSP vitro as well rodent model AD. Based our previous computational approach, was evaluated DKK1 binding, neuroprotective effects were examined using SHSY5Y cells, while activation analyzed through RTPCR HEK293/LRP6 cell line. Rodent AD developed intracerebroventricular administration Aβ25-35. Male Wistar rats randomly assigned receive oral doses (1 10 mg/kg) 28 days, after which behavioral assessments, biochemical analyses, RT-PCR, histopathological evaluations conducted. showed significant reduction property LRP6 overexpressed HEK293 cells. There decrease island latency treated lower dose (p < 0.01) higher 0.05) when compared control rats. Similarly, tests, significantly improved cognition. The deposition amyloid plaques found be considerably more got reduced treatment groups observed slides stained Congo red. Significant alterations mRNA levels protein expression glycogen synthase kinase-β (GSK-3β), β-catenin (β-cat) rat brain homogenates indicating upregulation WSP. In conclusion, demonstrated potential line cognitive benefits Furthermore, its ability activate highlights immense therapeutic enhance memory modulate key mechanisms.

Language: Английский

The miR-21-5p/DUSP8/MAPK signaling pathway mediates inflammation and apoptosis in vascular endothelial cells induced by intermittent hypoxia and contributes to the protective effects of N-acetylcysteine DOI
Yan Zhao, Renjun Lv,

Yao He

et al.

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: 997, P. 177462 - 177462

Published: March 7, 2025

Language: Английский

Citations

1
Voglibose Attenuates Amyloid Beta–Induced Memory Deficits in a Rodent Model: A Potential Alzheimer’s Therapy via Wnt Signaling Modulation DOI Creative Commons
Suman Manandhar, Prasada Chowdari Gurram, Anusha Govindula

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 17, 2025

Disruption of the Wnt signaling pathway (WSP), a highly conserved essential for growth and organ development, has been proven to play role in pathogenesis Alzheimer's disease (AD). This study focused on repurposing FDA-approved drug, Voglibose target DKK1-LRP6 site with goal upregulating WSP vitro as well rodent model AD. Based our previous computational approach, was evaluated DKK1 binding, neuroprotective effects were examined using SHSY5Y cells, while activation analyzed through RTPCR HEK293/LRP6 cell line. Rodent AD developed intracerebroventricular administration Aβ25-35. Male Wistar rats randomly assigned receive oral doses (1 10 mg/kg) 28 days, after which behavioral assessments, biochemical analyses, RT-PCR, histopathological evaluations conducted. showed significant reduction property LRP6 overexpressed HEK293 cells. There decrease island latency treated lower dose (p < 0.01) higher 0.05) when compared control rats. Similarly, tests, significantly improved cognition. The deposition amyloid plaques found be considerably more got reduced treatment groups observed slides stained Congo red. Significant alterations mRNA levels protein expression glycogen synthase kinase-β (GSK-3β), β-catenin (β-cat) rat brain homogenates indicating upregulation WSP. In conclusion, demonstrated potential line cognitive benefits Furthermore, its ability activate highlights immense therapeutic enhance memory modulate key mechanisms.

Language: Английский

Citations

0