
Inflammation, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 14, 2025
Atopic dermatitis (AD) is a multifaceted inflammatory skin condition characterized by the involvement of various cell types, such as keratinocytes, macrophages, neutrophils, and mast cells. Research indicates that flavonoids possess anti-inflammatory properties may be beneficial in management AD. However, investigation glycoside forms for anti-AD therapy limited. We aimed to assess ability quercetin-3-O-glycosides treating AD-like lesions through silico-, cell-, animal-based platforms. The glycosylated flavonols quercitrin, isoquercitrin, rutin were used this study. also tried understand influence glycone type on bioactivity delivery glycosides. glycosides effectively reduced overexpression proinflammatory effectors interleukin (IL)-6, chemokine (C-X-C motif) ligand (CXCL)1, CXCL8, regulated upon activation normal T expressed secreted (RANTES), thymus activation-regulated (TARC) activated keratinocytes. This reduction could due inhibition extracellular signal-regulated kinase (ERK) p38 phosphorylation. Isoquercitrin (but not quercitrin rutin) arrest upregulated IL-6 CCL5 macrophage model. significantly prevented histamine release from RBL-2H3 absorption examination showed greater permeation isoquercitrin than with dual sugar moieties smaller molecular volume higher lipophilicity. deposition was enhanced about 11-fold stripped delipidized skins, which mimicked AD lesions. vivo dinitrochlorobenzene (DNCB)-induced mouse model demonstrated less erosion, scaling, epidermal hyperplasia after topical treatment. concentration cytokines/chemokines lesion decreased isoquercitrin. These effects similar those tacrolimus ointment. immunohistochemistry (IHC) displayed hyperproliferation immune infiltration results indicated quercetin provide an efficient safe way treat inflammation.
Language: Английский