Alignment and characterization of remote-refocusing systems DOI Creative Commons
Wenzhi Hong, Hugh Sparks,

Chris Dunsby

et al.

Applied Optics, Journal Year: 2023, Volume and Issue: 62(28), P. 7431 - 7431

Published: Sept. 7, 2023

The technique of remote refocusing is used in optical microscopy to provide rapid axial scanning without mechanically perturbing the sample and techniques such as oblique plane that build on image a tilted within sample. magnification between pupils primary (O1) secondary (O2) microscope objectives remote-refocusing system has been shown previously by Mohanan Corbett [ J. Microsc. 288 , 95 ( 2022 ) JMICAR 0022-2720 10.1111/jmi.12991 ] be crucial obtaining broadest possible range. In this work, we performed an initial alignment then studied effect misalignments O1 O2, misalignment tube lens (TL1) relative (TL2), lateral TL2, changes focal length TL2. For each instance setup, measured mean point spread function FWHM xy 100 nm fluorescent beads normalized bead integrated fluorescence signal, calculated distortion system; all these quantities were mapped over range position. This allowed us estimate volume which diffraction-limited performance achieved how with system.

Language: Английский

Optoelectronic graded neurons for bioinspired in-sensor motion perception DOI
Jiewei Chen, Zheng Zhou, Beom Jin Kim

et al.

Nature Nanotechnology, Journal Year: 2023, Volume and Issue: 18(8), P. 882 - 888

Published: April 20, 2023

Language: Английский

Citations

194
How Flies See Motion DOI Creative Commons
Alexander Borst, Lukas N. Groschner

Annual Review of Neuroscience, Journal Year: 2023, Volume and Issue: 46(1), P. 17 - 37

Published: July 10, 2023

How neurons detect the direction of motion is a prime example neural computation: Motion vision found in visual systems virtually all sighted animals, it important for survival, and requires interesting computations with well-defined linear nonlinear processing steps—yet whole process moderate complexity. The genetic methods available fruit fly Drosophila charting connectome its system have led to rapid progress unprecedented detail our understanding how compute this organism. picture that emerged incorporates not only identity, morphology, synaptic connectivity each neuron involved but also neurotransmitters, receptors, their subcellular localization. Together neurons’ membrane potential responses stimulation, information provides basis biophysically realistic model circuit computes motion.

Language: Английский

Citations

22
Three-photon excited fluorescence imaging in neuroscience: From principles to applications DOI Creative Commons

Yujie Xiao,

Peng Deng,

Yaoguang Zhao

et al.

Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: Feb. 20, 2023

The development of three-photon microscopy (3PM) has greatly expanded the capability imaging deep within biological tissues, enabling neuroscientists to visualize structure and activity neuronal populations with greater depth than two-photon imaging. In this review, we outline history physical principles 3PM technology. We cover current techniques for improving performance 3PM. Furthermore, summarize applications various brain regions species. Finally, discuss future neuroscience.

Language: Английский

Citations

17
Recent advances in oblique plane microscopy DOI Creative Commons
Jeongmin Kim

Nanophotonics, Journal Year: 2023, Volume and Issue: 12(13), P. 2317 - 2334

Published: April 20, 2023

Abstract Oblique plane microscopy (OPM) directly captures object information in a tilted from the focal of objective lens without need for slow z-stack acquisition. This unconventional widefield imaging approach is made possible by using remote focusing principle that eliminates optical aberrations points beyond plane. Together with oblique lightsheet illumination, OPM can make conventional fully compatible standard biological specimens prepared on microscope slides. not only an excellent high-speed volumetric platform sweeping illumination mechanically moving either sample or space, but also provides solution direct along any orientation interest single shot. Since its first demonstration 2008, has continued to evolve into advanced biological, medical, and materials science applications. In recent years, many technological advances have been goal super-resolution, fast imaging, large field view, etc. review gives overview OPM’s working performance introduces technical developments methods strong potential variety research fields, including cellular developmental biology, clinical diagnostics histology ophthalmology, flow cytometry, microfluidic devices, soft materials.

Language: Английский

Citations

16
Alignment and Characterisation of Remote-Refocusing Systems DOI Creative Commons
Wenzhi Hong, Hugh Sparks,

Chris Dunsby

et al.

arXiv (Cornell University), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

The technique of remote refocusing is used in optical microscopy to provide rapid axial scanning without mechanically perturbing the sample and techniques such as oblique plane that build on image a tilted within sample. magnification between pupils primary (O1) secondary (O2) microscope objectives remote-refocusing system has been shown previously by Mohanan Corbett [J. Microsc.288, 95 (2022)JMICAR0022-272010.1111/jmi.12991] be crucial obtaining broadest possible range. In this work, we performed an initial alignment then studied effect misalignments O1 O2, misalignment tube lens (TL1) relative (TL2), lateral TL2, changes focal length TL2. For each instance setup, measured mean point spread function

Language: Английский

Citations

4
Alignment and characterization of remote-refocusing systems DOI Creative Commons
Wenzhi Hong, Hugh Sparks,

Chris Dunsby

et al.

Applied Optics, Journal Year: 2023, Volume and Issue: 62(28), P. 7431 - 7431

Published: Sept. 7, 2023

The technique of remote refocusing is used in optical microscopy to provide rapid axial scanning without mechanically perturbing the sample and techniques such as oblique plane that build on image a tilted within sample. magnification between pupils primary (O1) secondary (O2) microscope objectives remote-refocusing system has been shown previously by Mohanan Corbett [ J. Microsc. 288 , 95 ( 2022 ) JMICAR 0022-2720 10.1111/jmi.12991 ] be crucial obtaining broadest possible range. In this work, we performed an initial alignment then studied effect misalignments O1 O2, misalignment tube lens (TL1) relative (TL2), lateral TL2, changes focal length TL2. For each instance setup, measured mean point spread function FWHM xy 100 nm fluorescent beads normalized bead integrated fluorescence signal, calculated distortion system; all these quantities were mapped over range position. This allowed us estimate volume which diffraction-limited performance achieved how with system.

Language: Английский

Citations

4