Molecules,
Journal Year:
2022,
Volume and Issue:
27(22), P. 7903 - 7903
Published: Nov. 15, 2022
Amino-terminal
CuII
and
NiII
(ATCUN)
binding
sequences
are
widespread
in
the
biological
world.
Here,
we
report
on
study
of
eight
ATCUN
peptides
aimed
at
targeting
copper
ions
stopping
associated
formation
reactive
oxygen
species
(ROS).
This
was
actually
more
focused
Cu(Aβ)-induced
ROS
production
which
Aβ
peptide
is
“villain”
linked
to
Alzheimer’s
disease.
The
full
characterization
peptides,
extraction
from
CuII(Aβ),
ability
prevent
and/or
stop
described
relevant
conditions.
We
highlighted
this
research
that
all
motifs
studied
formed
same
thermodynamic
complex
but
addition
a
second
histidine
position
1
or
2
allowed
for
an
improvement
uptake
kinetics.
kinetic
rate
directly
related
CuII(Aβ)-induced
ROS,
with
most
efficient
being
HWHG
HGHW.
Advanced Sensor Research,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 23, 2025
Abstract
Biorecognition
elements
immobilized
into
nanopores
have
transformed
point‐of‐care
(POC)
diagnostics
by
converting
molecular
interactions
electrical
and
fluorescent
signals.This
study
introduces
Bio‐Sensei,
a
high‐throughput
screening
(HTS)
microfluidic
platform
based
on
nanopore
biosensing.
Integrating
robotic
sampler,
electrochemical,
fluorescence
setup,
Bio‐Sensei
operates
as
an
Internet
of
Things
(IoT)
with
integrated
data
analysis.
The
platform's
utility
is
demonstrated
functionalized
amino
terminal
Cu(II)‐
Ni(II)‐binding
(ATCUN)
peptide
ion
track‐etched
membrane.
Automated
testing
atchieves
significantly
higher
F‐stat
value
than
the
critical
treshold,
while
unsupervised
clustering
reveals
optimalnanopores
pore
size.
biosensordemonstrates
remarkable
stability,
selectivity,
sensitivity
detection
limits
10
−6
using
−15
Musing
cyclic
voltammetry
measurements.
Combining
these
methods
enhances
machine
learning
models
for
Cu
2+
concentration
prediction,
achieving
receiver
operating
characteristic
area
under
curve
values
exceeding
95%.
Biotechnology Advances,
Journal Year:
2025,
Volume and Issue:
unknown, P. 108570 - 108570
Published: March 1, 2025
This
review
provides
a
comprehensive
analysis
of
antimicrobial
peptides
(AMPs),
exploring
their
diverse
sources,
secondary
structures,
and
unique
characteristics.
The
explores
into
the
mechanisms
underlying
antibacterial,
immunomodulatory
effects,
antiviral,
antiparasitic
antitumour
AMPs.
Furthermore,
it
discusses
three
principal
synthesis
pathways
for
AMPs
assesses
current
clinical
applications
preclinical
research
status.
paper
also
addresses
limitations
AMPs,
including
issues
related
to
stability,
resistance,
toxicity,
while
offering
insights
strategies
enhancement.
Recent
advancements
in
AMP
research,
such
as
chemical
modifications
(including
amino
acid
sequence
optimisation,
terminal
side-chain
modifications,
PEGylation,
conjugation
with
small
molecules,
photosensitisers,
metal
ligands,
polymerisation,
cyclisation
specifically
targeted
peptides)
are
highlighted.
goal
is
provide
foundation
future
design
optimisation
Chemistry - A European Journal,
Journal Year:
2022,
Volume and Issue:
28(23)
Published: Jan. 26, 2022
Abstract
Sulfite
is
a
potent
toxic
substance
causing
harm
to
multi‐organ
in
human.
Despite
toxicity,
it
widely
used
as
preservative,
anti‐browning
and
anti‐oxidant
foods,
beverages,
pharmaceuticals,
which
cause
easy
admission
of
sulfite
also
produced
endogenously
during
the
catabolism
cysteine
methionine.
In
vivo,
serum
level
at
physiological
range
strictly
maintained
by
molybdenum
dependent
oxidase
(SO),
catalyzes
sulfate
oxidation
via
two‐electron
pathway.
The
loss
SO
activity
causes
high
that
fosters
several
diseases,
including
asthma,
neurological
dysfunction,
birth
defects,
heart
diseases.
cytotoxicity
(bi)sulfite
implicated
radicals,
are
generated
mainly
heme‐peroxidases
one‐electron
On
other
hand,
radicals
neutralized
heme‐globins.
enzymatic
reduction
sulfide
catalyzed
reductase,
contains
an
unusual
metal
cofactor,
siroheme‐[4Fe4S]‐cluster.
Overall,
interaction
with
various
metalloproteins
vivo
close
relation
human
health.
Therefore,
this
review
describes
metabolic
conversion
sulfate,
radical
or
pathways
(specially
SOs,
peroxidases,
heme‐globins,
reductases),
potential
applications
biosensors/biofuel
cells,
anti‐browning,
advance
process.
Journal of Inorganic Biochemistry,
Journal Year:
2023,
Volume and Issue:
244, P. 112203 - 112203
Published: March 30, 2023
Transition
metal
ions
such
as
iron,
copper,
zinc,
manganese
or,
nickel
are
essential
in
many
biological
processes.
Bacteria
have
developed
a
number
of
mechanisms
for
their
acquisition
and
transport,
which
numerous
proteins
smaller
molecules
involved.
One
the
representatives
these
is
FeoB,
belongs
to
Feo
(ferrous
ion
transporter)
family.
Although
ferrous
iron
transport
system
widespread
among
microorganisms,
it
still
poorly
described
Gram-positive
pathogens,
Staphylococcus
aureus.
In
this
work,
combined
potentiometric
spectroscopic
studies
(UV-Vis,
CD
EPR)
were
carried
out
determine
Cu(II),
Fe(II)
Zn(II)
binding
modes
FeoB
fragments
(Ac-IDYHKLMK-NH2,
Ac-ETSHDKY-NH2,
Ac-SFLHMVGS-NH2).
For
first
time
iron(II)
complexes
with
peptides
characterized
by
potentiometry.
All
studied
ligands
able
form
variety
thermodynamically
stable
transition
ions.
It
was
concluded
that
systems,
most
effective
observed
Ac-ETSHDKY-NH2
peptide.
Moreover,
comparing
preferences
all
towards
different
ions,
copper(II)
ones
at
physiological
pH.
Chemistry - A European Journal,
Journal Year:
2021,
Volume and Issue:
27(72), P. 18093 - 18102
Published: Oct. 20, 2021
Abstract
Redox‐active
Cu(II)
complexes
are
able
to
form
reactive
oxygen
species
(ROS)
in
the
presence
of
and
reducing
agents.
Recently,
Faller
et
al.
reported
that
ROS
generation
by
ATCUN
is
not
as
high
assumed
for
decades.
High
complex
stability
results
silencing
Cu(II)/Cu(I)
redox
cycle
therefore
leads
low
generation.
In
this
work,
we
demonstrate
an
exchange
α‐amino
acid
Gly
with
β‐amino
β‐Ala
at
position
2
(Gly2→β‐Ala2)
motif
reinstates
production
(
•
OH
H
O
).
Potentiometry,
cyclic
voltammetry,
EPR
spectroscopy
DFT
simulations
were
utilized
explain
increased
these
β‐Ala2‐containing
complexes.
We
also
observed
enhanced
oxidative
cleavage
activity
towards
plasmid
DNA
β‐Ala2
compared
Gly2
Modifications
positively
charged
Lys
residues
affinity
through
electrostatic
interactions
determined
UV/VIS,
fluorescence,
CD
spectroscopy,
consequently
led
a
further
increase
nuclease
activity.
A
similar
trend
was
regarding
cytotoxic
against
several
human
cancer
cell
lines
where
peptide
had
lower
IC
50
values
Gly2.
The
higher
cytotoxicity
could
be
attributed
cellular
uptake
ICP‐MS
measurements.