Sequence–Activity Relationship of ATCUN Peptides in the Context of Alzheimer’s Disease DOI Creative Commons
Margot Lefèvre, Kyangwi P. Malikidogo, Charlène Esmieu

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(22), P. 7903 - 7903

Published: Nov. 15, 2022

Amino-terminal CuII and NiII (ATCUN) binding sequences are widespread in the biological world. Here, we report on study of eight ATCUN peptides aimed at targeting copper ions stopping associated formation reactive oxygen species (ROS). This was actually more focused Cu(Aβ)-induced ROS production which Aβ peptide is “villain” linked to Alzheimer’s disease. The full characterization peptides, extraction from CuII(Aβ), ability prevent and/or stop described relevant conditions. We highlighted this research that all motifs studied formed same thermodynamic complex but addition a second histidine position 1 or 2 allowed for an improvement uptake kinetics. kinetic rate directly related CuII(Aβ)-induced ROS, with most efficient being HWHG HGHW.

Language: Английский

Peptide-based probe for colorimetric and fluorescent detection of Cu2+ and S2- in environmental and biological systems DOI
Yong An,

Linyu Li,

Lepeng Li

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 465, P. 133192 - 133192

Published: Dec. 6, 2023

Language: Английский

Citations

43
Automated Microfluidic Platform for High‐Throughput Biosensor Development DOI Creative Commons
Shitanshu Devrani, Daniel Tietze, Alesia A. Tietze

et al.

Advanced Sensor Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Biorecognition elements immobilized into nanopores have transformed point‐of‐care (POC) diagnostics by converting molecular interactions electrical and fluorescent signals.This study introduces Bio‐Sensei, a high‐throughput screening (HTS) microfluidic platform based on nanopore biosensing. Integrating robotic sampler, electrochemical, fluorescence setup, Bio‐Sensei operates as an Internet of Things (IoT) with integrated data analysis. The platform's utility is demonstrated functionalized amino terminal Cu(II)‐ Ni(II)‐binding (ATCUN) peptide ion track‐etched membrane. Automated testing atchieves significantly higher F‐stat value than the critical treshold, while unsupervised clustering reveals optimalnanopores pore size. biosensordemonstrates remarkable stability, selectivity, sensitivity detection limits 10 −6 using −15 Musing cyclic voltammetry measurements. Combining these methods enhances machine learning models for Cu 2+ concentration prediction, achieving receiver operating characteristic area under curve values exceeding 95%.

Language: Английский

Citations

1
Identification of Ni2+-binding peptides in sunflower meal protein hydrolysate for deeper understanding of peptide-metal interactions DOI Creative Commons
Jairo Andrés Camaño Echavarría,

Christelle Mathé,

Jean‐Michel Girardet

et al.

Journal of Inorganic Biochemistry, Journal Year: 2025, Volume and Issue: 269, P. 112877 - 112877

Published: March 5, 2025

Language: Английский

Citations

1
Advances in antimicrobial peptides: From mechanistic insights to chemical modifications DOI Creative Commons
Yang Ren, Xiaohan Ma, Feng Peng

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108570 - 108570

Published: March 1, 2025

This review provides a comprehensive analysis of antimicrobial peptides (AMPs), exploring their diverse sources, secondary structures, and unique characteristics. The explores into the mechanisms underlying antibacterial, immunomodulatory effects, antiviral, antiparasitic antitumour AMPs. Furthermore, it discusses three principal synthesis pathways for AMPs assesses current clinical applications preclinical research status. paper also addresses limitations AMPs, including issues related to stability, resistance, toxicity, while offering insights strategies enhancement. Recent advancements in AMP research, such as chemical modifications (including amino acid sequence optimisation, terminal side-chain modifications, PEGylation, conjugation with small molecules, photosensitisers, metal ligands, polymerisation, cyclisation specifically targeted peptides) are highlighted. goal is provide foundation future design optimisation

Language: Английский

Citations

1
Intercommunication between metal ions and amyloidogenic peptides or proteins in protein misfolding disorders DOI
Jong‐Min Suh, Mingeun Kim,

Jeasang Yoo

et al.

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 478, P. 214978 - 214978

Published: Dec. 16, 2022

Language: Английский

Citations

32
Cross‐talk Between (Hydrogen)Sulfite and Metalloproteins: Impact on Human Health DOI
Biplab K. Maiti

Chemistry - A European Journal, Journal Year: 2022, Volume and Issue: 28(23)

Published: Jan. 26, 2022

Abstract Sulfite is a potent toxic substance causing harm to multi‐organ in human. Despite toxicity, it widely used as preservative, anti‐browning and anti‐oxidant foods, beverages, pharmaceuticals, which cause easy admission of sulfite also produced endogenously during the catabolism cysteine methionine. In vivo, serum level at physiological range strictly maintained by molybdenum dependent oxidase (SO), catalyzes sulfate oxidation via two‐electron pathway. The loss SO activity causes high that fosters several diseases, including asthma, neurological dysfunction, birth defects, heart diseases. cytotoxicity (bi)sulfite implicated radicals, are generated mainly heme‐peroxidases one‐electron On other hand, radicals neutralized heme‐globins. enzymatic reduction sulfide catalyzed reductase, contains an unusual metal cofactor, siroheme‐[4Fe4S]‐cluster. Overall, interaction with various metalloproteins vivo close relation human health. Therefore, this review describes metabolic conversion sulfate, radical or pathways (specially SOs, peroxidases, heme‐globins, reductases), potential applications biosensors/biofuel cells, anti‐browning, advance process.

Language: Английский

Citations

29
Nuclease-like metalloscissors: Biomimetic candidates for cancer and bacterial and viral infections therapy DOI

Marzieh Anjomshoa,

Bagher Amirheidari

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 458, P. 214417 - 214417

Published: Feb. 5, 2022

Language: Английский

Citations

27
Rational design of site-specific artificial metallonucleases for therapeutic applications DOI Creative Commons
Xixi Lu, Chengchen Zhang

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113736 - 113736

Published: April 1, 2025

Language: Английский

Citations

0
Divalent metal ion binding to Staphylococcus aureus FeoB transporter regions DOI Creative Commons
Valentyn Dzyhovskyi, Kamila Stokowa‐Sołtys

Journal of Inorganic Biochemistry, Journal Year: 2023, Volume and Issue: 244, P. 112203 - 112203

Published: March 30, 2023

Transition metal ions such as iron, copper, zinc, manganese or, nickel are essential in many biological processes. Bacteria have developed a number of mechanisms for their acquisition and transport, which numerous proteins smaller molecules involved. One the representatives these is FeoB, belongs to Feo (ferrous ion transporter) family. Although ferrous iron transport system widespread among microorganisms, it still poorly described Gram-positive pathogens, Staphylococcus aureus. In this work, combined potentiometric spectroscopic studies (UV-Vis, CD EPR) were carried out determine Cu(II), Fe(II) Zn(II) binding modes FeoB fragments (Ac-IDYHKLMK-NH2, Ac-ETSHDKY-NH2, Ac-SFLHMVGS-NH2). For first time iron(II) complexes with peptides characterized by potentiometry. All studied ligands able form variety thermodynamically stable transition ions. It was concluded that systems, most effective observed Ac-ETSHDKY-NH2 peptide. Moreover, comparing preferences all towards different ions, copper(II) ones at physiological pH.

Language: Английский

Citations

8
Incorporation of β‐Alanine in Cu(II) ATCUN Peptide Complexes Increases ROS Levels, DNA Cleavage and Antiproliferative Activity** DOI Creative Commons
Julian Heinrich, Karolina Bossak‐Ahmad, Mie Riisom

et al.

Chemistry - A European Journal, Journal Year: 2021, Volume and Issue: 27(72), P. 18093 - 18102

Published: Oct. 20, 2021

Abstract Redox‐active Cu(II) complexes are able to form reactive oxygen species (ROS) in the presence of and reducing agents. Recently, Faller et al. reported that ROS generation by ATCUN is not as high assumed for decades. High complex stability results silencing Cu(II)/Cu(I) redox cycle therefore leads low generation. In this work, we demonstrate an exchange α‐amino acid Gly with β‐amino β‐Ala at position 2 (Gly2→β‐Ala2) motif reinstates production ( • OH H O ). Potentiometry, cyclic voltammetry, EPR spectroscopy DFT simulations were utilized explain increased these β‐Ala2‐containing complexes. We also observed enhanced oxidative cleavage activity towards plasmid DNA β‐Ala2 compared Gly2 Modifications positively charged Lys residues affinity through electrostatic interactions determined UV/VIS, fluorescence, CD spectroscopy, consequently led a further increase nuclease activity. A similar trend was regarding cytotoxic against several human cancer cell lines where peptide had lower IC 50 values Gly2. The higher cytotoxicity could be attributed cellular uptake ICP‐MS measurements.

Language: Английский

Citations

15