Automating Predictive Phage Therapy Pharmacology

Antibiotics, Journal Year: 2023, Volume and Issue: 12(9), P. 1423 - 1423

Published: Sept. 8, 2023

Viruses that infect as well often kill bacteria are called bacteriophages, or phages. Because of their ability to act bactericidally, phages increasingly being employed clinically antibacterial agents, an infection-fighting strategy has been in practice now for over one hundred years. As with agents generally, the development this phage therapy can be aided via application various quantitative frameworks. Therefore, reviewed here considerations multiplicity infection, bacterial likelihood becoming adsorbed a function titers, susceptibility also and use Poisson distributions predict impacts on bacteria. Considered addition is simulations take into account both replication. These approaches automated, i.e., by employing number online-available apps provided author, which review emphasizes. In short, mathematical whose implementation eased online automation.

Language: Английский

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Bacteriophages in the fish mucosa: updates on their biology, interactions, and applications

Biologia, Journal Year: 2024, Volume and Issue: 79(6), P. 1861 - 1873

Published: March 12, 2024

Language: Английский

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Biophysical Breakthroughs Projected for the Phage Therapy of Bacterial Disease

Biophysica, Journal Year: 2024, Volume and Issue: 4(2), P. 195 - 206

Published: April 12, 2024

Past anti-bacterial use of bacteriophages (phage therapy) is already well reviewed as a potential therapeutic response to the emergence multidrug-resistant, pathogenic bacteria. Phage therapy has been limited by following. (1) The success rate too low for routine and Food Drug Administration (FDA) approval. (2) Current strategies phage characterization do not sufficiently improve therapy. (3) stability many phages at ambient temperature high enough routinely store transport temperature. In present communication, we new previous data that interpret introductory biophysically efficiently transforming needed level effectiveness. Included are procedure preliminary native gel electrophoresis (a low-cost procedure) projecting effectiveness newly isolated phage, suggest way achieve stabilizing dried, ambient-temperature via polymer embedding, means increase blood persistence, therefore effectiveness, what would otherwise be relatively low-persistence phage.

Language: Английский

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The role of bacteriophages in shaping bacterial composition and diversity in the human gut

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 19, 2023

The microbiota of the gut has continued to co-evolve alongside their human hosts conferring considerable health benefits including production nutrients, drug metabolism, modulation immune system, and playing an antagonistic role against pathogen invasion gastrointestinal tract (GIT). is said provide a habitat for diverse groups microorganisms where they all co-habit interact with one another system humans. Phages are bacterial parasites that require host metabolic replicate via lytic or lysogenic cycle. phage populations regarded as most dominant in ecosystem. As such, among various microbial interactions, phage-bacteria although complex, have been demonstrated over time using different mechanisms such predation, conversion, induction, counterdefense by population. With help models dynamics complexity behind survival ecosystem was demystified, roles maintaining homeostasis promoting overall humans were elucidated. Although treatment infections be successful multidrug-resistant causative agents, concerns about this technique still very much alive researchers owing potential phages evolve. Since dearth knowledge exists regarding use therapeutic purposes, more studies involving experimental clinical trials needed widen understanding bacteria-phage interactions association immunological responses

Language: Английский

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Effective treatment of a broad-host-range lytic phage SapYZU15 in eliminating Staphylococcus aureus from subcutaneous infection

Microbiological Research, Journal Year: 2023, Volume and Issue: 276, P. 127484 - 127484

Published: Aug. 30, 2023

Language: Английский

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Bacterial Pathogens in Food and Their Control by Bacteriophages

Advances in environmental engineering and green technologies book series, Journal Year: 2024, Volume and Issue: unknown, P. 175 - 228

Published: Jan. 17, 2024

Food safety ensures reduced food losses and therefore contributes to security. The factors causing the contamination of may threaten safe consumption make foods harmful. For this reason, it is necessary utilize various resources prevent from being contaminated in all stages chain. Purified high titer phage lysates have been used for species-specific control bacteria during pre postharvest phases production storage. This chapter presents recent advances bacteriophage research their application area safety. Some components are general facts on biology that relevant detection bacterial pathogens environmental samples, bacteriophages growth spoilage organisms processing environment, identification pathogens, advantages bacteriophage-based methods shortcomings.

Language: Английский

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Quantifying Plant Signaling Pathways by Integrating Bacterial-Based Sensors and Mathematical Modeling for Feedback Networks

Published: Jan. 1, 2024

Language: Английский

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Phytopathological management through bacteriophages: enhancing food security amidst climate change

Journal of Industrial Microbiology & Biotechnology, Journal Year: 2024, Volume and Issue: 51

Published: Jan. 1, 2024

Abstract The increasing global population and climate change pose significant challenges to agriculture, particularly in managing plant diseases caused by phytopathogens. Traditional methods, including chemical pesticides antibiotics, have become less effective due pathogen resistance environmental concerns. Phage therapy emerges as a promising alternative, offering sustainable precise approach controlling bacterial without harming beneficial soil microorganisms. This review explores the potential of bacteriophages biocontrol agents, highlighting their specificity, rapid multiplication, minimal impact. We discuss historical context, current applications, prospects phage emphasizing its role enhancing crop yield quality. Additionally, paper examines integration with modern agricultural practices development cocktails genetically engineered phages combat resistant pathogens. findings suggest that could revolutionize phytopathological management, contributing food security practices. One-Sentence Summary burden phage-based treatment.

Language: Английский

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Multi-strain phage induced clearance of bacterial infections

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 7, 2024

Bacteriophage (or ‘phage’ – viruses that infect and kill bacteria) are increasingly considered as a therapeutic alternative to treat antibiotic-resistant bacterial infections. However, bacteria can evolve resistance phage, presenting significant challenge the near- long-term success of phage therapeutics. Application mixtures multiple (i.e., ‘cocktails’) have been proposed limit emergence phage-resistant mutants could lead failure. Here, we combine theory computational models in vivo therapy study efficacy cocktail, composed two complementary phages motivated by example Pseudomonas aeruginosa facing exploit different surface receptors, LUZ19v PAK P1. As confirmed Luria-Delbrück fluctuation test, this motivating serves model for instances where extremely unlikely develop simultaneous mutations against both phages. We then quantify outcomes given single- or double-phage treatment models, function traits host immune strength. Building upon prior work showing monophage immunocompetent hosts, here show cocktails comprised targeting independent receptors improve outcome immunocompromised hosts reduce chance pathogens simultaneously combinations. The finding cocktail is qualitatively robust differences virus-bacteria interactions dynamics. Altogether, combined use analysis highlights influence viral life history receptor complementarity when designing deploying hosts.

Language: Английский

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Phage-induced disturbance of a marine sponge microbiome

Environmental Microbiome, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 26, 2024

Abstract Background Bacteriophages are known modulators of community composition and activity in environmental host-associated microbiomes. However, the impact single phages have on bacterial dynamics under viral predation, extent duration their effect, not completely understood. In this study, we combine morphological genomic characterization a novel marine phage, isolated from Baltic sponge Halichondria panicea , report first attempts controlled phage-manipulation natural sponge-associated Results We used culture-based culture-independent (16S rRNA gene amplicon sequencing) methods to investigate microbiomes with without addition phages. Upon application Maribacter specialist phage Panino conditions, were able detect community-wide shifts microbiome load after 72 h. While became more dissimilar over time presence phages, species evenness richness maintained. exposure, observed loss several low-abundance constituent taxa resident microbiota, while other originally underrepresented increased. Virulent likely induce disturbances, evident changes total microbial profile by specific elimination taxa, which leads an increase abundance opportunistic such as genera Vibrio Pseudoalteromonas Photobacterium . Conclusions Our findings suggest that diversity and, extension, its resilience depend maintenance members, irrespective abundance. Phage-induced disturbances can significantly alter structure promoting growth bacteria like shifting dysbiotic state. These insights highlight role bacteriophages shaping underscore potential for managing environments.

Language: Английский

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