Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Xinjiang, China, from 2020 to 2022

Archives of Virology, Journal Year: 2024, Volume and Issue: 169(5)

Published: April 15, 2024

Language: Английский

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Towards a Safer Future: Enhancing Vaccine Development to Combat Animal Coronaviruses

Vaccines, Journal Year: 2024, Volume and Issue: 12(3), P. 330 - 330

Published: March 19, 2024

Coronaviruses (CoVs) are a large class of positively stranded RNA viruses that pose significant threat to public health, livestock farming, and wild animals. These have the ability cross species barriers cause devastating epidemics. Animals considered be intermediate hosts for many coronaviruses, animal coronaviruses also potential cross-species transmission humans. Therefore, controlling epidemic is great importance human health. Vaccination programs proven effective in infections, offering cost-effective approach reducing morbidity mortality, so re-emergence lethal emphasizes urgent need development vaccines. In this regard, we explore progress coronavirus vaccine development, covering latest taxonomy main spillover events, diverse platforms, targets primary challenges facing We emphasize create “dual-effect” capable eliciting both cellular humoral immune responses. The goal highlight contributions veterinary scientists field interdisciplinary collaboration between medical communities. By promoting communication cooperation, can enhance novel super vaccines combat infections future.

Language: Английский

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Genome-scale CRISPR screen identifies TRIM2 and SLC35A1 associated with porcine epidemic diarrhoea virus infection

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 250, P. 125962 - 125962

Published: July 25, 2023

Language: Английский

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NS7a of SADS-CoV promotes viral infection via inducing apoptosis to suppress type III interferon production

Journal of Virology, Journal Year: 2024, Volume and Issue: 98(5)

Published: April 16, 2024

ABSTRACT Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered swine with potential cross-species transmission risk. Although SADS-CoV-induced host cell apoptosis and innate immunity antagonization has been revealed, underlying signaling pathways remain obscure. Here, we demonstrated that infection of SADS-CoV induced in vivo vitro , viral protein NS7a mainly responsible for cells. Furthermore, found interacted apoptosis-inducing factor mitochondria associated 1 (AIFM1) to activate caspase-3 via caspase-6 SADS-CoV-infected cells, enhanced replication. Importantly, suppressed poly(I:C)-induced expression type III interferon (IFN-λ) activating cleave regulatory 3 (IRF3), inhibitor protects piglets against . These findings reveal how inhibit provide valuable clue the development effective drugs clinical control infection. IMPORTANCE Over last 20 years, multiple animal-originated coronaviruses, including severe respiratory (SARS-CoV), middle east (MERS-CoV), SARS-CoV-2, have caused millions deaths, seriously jeopardized human health, hindered social development, indicating study coronaviruses particularly important. Bat-originated (SADS-CoV), 2017, can not only cause fatal piglets, but also infect risk transmission, its pathogenesis unclear. In this study, suppresses IFN-λ production (AIFM1)-caspase-6-caspase-3-interferon (IRF3) pathway, (Z-DEVD-FMK) effectively replication protect infected piglets. Our contribute better understanding SADS-CoV-host interactions as part using apoptosis-inhibitor drug therapeutic approaches prevention

Language: Английский

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Towards developing multistrain PEDV vaccines: Integrating basic concepts and SARS-CoV-2 pan-sarbecovirus strategies

Virology, Journal Year: 2025, Volume and Issue: 604, P. 110412 - 110412

Published: Jan. 19, 2025

Language: Английский

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Salinomycin inhibits porcine epidemic diarrhea virus infection by targeting Wnt/β-catenin pathway

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 155, P. 114652 - 114652

Published: April 10, 2025

Language: Английский

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Subcellular localization of viral proteins after porcine epidemic diarrhea virus infection and their roles in the viral life cycle

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 274, P. 133401 - 133401

Published: June 24, 2024

Language: Английский

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Accurate location of two conserved linear epitopes of PEDV utilizing monoclonal antibodies induced by S1 protein nanoparticles

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 127276 - 127276

Published: Oct. 6, 2023

Language: Английский

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Diversity for endoribonuclease nsp15-mediated regulation of alpha-coronavirus propagation and virulence

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(6)

Published: Nov. 8, 2023

Understanding the role of endoribonuclease non-structural protein 15 (nsp15) (EndoU) in coronavirus (CoV) infection and pathogenesis is essential for vaccine target discovery. Whether EndoU activity CoV nsp15, as a virulence-related protein, has diverse effect on viral virulence needs to be further explored. Here, we found that transmissible gastroenteritis virus (TGEV) feline infectious peritonitis (FIPV) nsp15 proteins antagonize SeV-induced interferon-β (IFN-β) production human embryonic kidney 293 cells. Interestingly, compared with wild-type infection, EnUmt-TGEV or EnUmt-FIPV did not change IFN-β response reduce propagation immunocompetent The results animal experiments showed EnUmt viruses clinical presentation mortality caused by TGEV FIPV. Our findings enrich understanding nsp15-mediated regulation alpha-CoV reveal conserved functions nonstructural have effects pathogenicity CoVs.

Language: Английский

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Identification of Three Novel Linear B-Cell Epitopes in Non-Structural Protein 3 of Porcine Epidemic Diarrhea Virus Using Monoclonal Antibodies

Viruses, Journal Year: 2024, Volume and Issue: 16(3), P. 424 - 424

Published: March 9, 2024

Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic swine coronavirus that causes and high mortality in piglets, resulting significant economic losses within the global industry. Nonstructural protein 3 (Nsp3) largest coronavirus, playing critical roles viral replication, such as processing of polyproteins formation replication-transcription complexes (RTCs). In this study, three monoclonal antibodies (mAbs), 7G4, 5A3, 2D7, targeting PEDV Nsp3 were successfully generated, distinct linear B-cell epitopes identified these mAbs by using Western blotting analysis with 24 truncations Nsp3. The epitope against 7G4 was located on amino acids 31-TISQDLLDVE-40, 5A3 found 141-LGIVDDPAMG-150, 2D7 situated 282-FYDAAMAIDG-291. Intriguingly, 31-TISQDLLDVE-40 recognized mAb appears to be due its antigenic index exposed location surface protein. addition, bioinformatics unveiled conserved most genotypes PEDV. These findings present first characterization novel provide potential tools for identifying host proteins may facilitate infection.

Language: Английский

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