A single pair of pharyngeal neurons functions as a commander to reject high salt inDrosophila melanogaster DOI Creative Commons
Jiun Sang, Subash Dhakal, Bhanu Shrestha

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 17, 2023

Abstract Salt is an essential nutrient for survival, while excessive NaCl can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in pharynx are critical gatekeepers impacting decision to accept or reject a food. Currently, our understanding of mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high salt rudimentary. Here, we found that member ionotropic family, Ir60b expressed exclusively pair GRNs activated by salt. Using two-way choice assay (DrosoX) measure ingestion volume, demonstrate IR60b and two coreceptors IR25a IR76b, required prevent consumption. Mutants lacking external but retaining exhibit much higher avoidance than flies with all missing three IRs. Our findings highlight vital role IRs GRN control

Language: Английский

Age-dependent switched taste behavior to ribose DOI
Jiun Sang, Youngseok Lee

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 174, P. 104194 - 104194

Published: Oct. 13, 2024

Language: Английский

Citations

2
A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster DOI Creative Commons
Jiun Sang, Subash Dhakal, Bhanu Shrestha

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: April 4, 2024

Salt (NaCl), is an essential nutrient for survival, while excessive salt can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in pharynx are critical gatekeepers impacting decision to accept or reject a food. Currently, our understanding of mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high rudimentary. Here, we found that member ionotropic family, Ir60b expressed exclusively pair GRNs activated by salt. Using two-way choice assay (DrosoX) measure ingestion volume, demonstrate IR60b and two co-receptors IR25a IR76b required prevent consumption. Mutants lacking external but retaining exhibit much higher avoidance than flies with all missing three IRs. Our findings highlight vital role IRs GRN control

Language: Английский

Citations

1
Disposable electrochemical aptasensor with mesoporous carbon spheres modified screen-printed dual-working electrodes for Pb2+ and Hg2+ detection DOI
Zhenping Liu,

F. P. Zhong,

Zihao Wu

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 422, P. 136558 - 136558

Published: Aug. 30, 2024

Language: Английский

Citations

1
<i>Drosophila melanogaster </i>as a Bioindicator in Comparative Copper and Lead Toxicology: Exploring the Health Implications of Heavy Metal Exposure DOI Open Access
Ioan Sărac,

Emilian Onisan,

I. Petrescu

et al.

Published: March 5, 2024

Drosophila melanogaster serves as a important research model both for genetic investigations and the study of metal toxicity, facilitating elucidation physiological mechanisms comparable to those human organisms. In our research, we evaluated toxicity effect resulting from exposure various concentrations lead copper on prolificity rates throughout life cycle (egg-adult) four genotypes melanogaster: wild-type (control), white, brown, white-vestigial. During study, were examined across three repetitions under impact 0.50, 1.00, 2.00, 4.00 mM (CuSO4) (Pb(C2H3O2)2). Prolificity exhibited variations direct consequence factors, concentration exposure, specific type metal, either or lead. The mutant white-vestigial genotype revealed an IC50 inhibition at lower doses 2.00 lead, in contrast control (wild-type), which rate &gt;IC50 only case copper. Our results concluded that (i) dose influences directly proportional manner, (ii) comparative analyses between displayed all within range 0.50 mM. contrast, 1.00 mM, highlighting (iii) more acute characteristic Thus, this reflect importance using interaction factors metals, offering significant insights into monitoring their defining maximum permissible

Language: Английский

Citations

1
A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster DOI Open Access
Jiun Sang, Subash Dhakal, Bhanu Shrestha

et al.

Published: March 20, 2024

Salt is an essential nutrient for survival, while excessive NaCl can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in pharynx are critical gatekeepers impacting decision to accept or reject a food. Currently, our understanding of mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high salt rudimentary. Here, we found that member ionotropic family, Ir60b expressed exclusively pair GRNs activated by salt. Using two-way choice assay (DrosoX) measure ingestion volume, demonstrate IR60b and two coreceptors IR25a IR76b, required prevent consumption. Mutants lacking external but retaining exhibit much higher avoidance than flies with all missing three IRs. Our findings highlight vital role IRs GRN control

Language: Английский

Citations

0
Pharyngeal neuronal mechanisms governing sour taste perception in Drosophila melanogaster DOI Open Access

Bhanu Shrestha,

Jiun Sang, Suman Rimal

et al.

Published: Oct. 2, 2024

Sour taste, which is elicited by low pH, may serve to help animals distinguish appetitive from potentially harmful food sources. In all species studied date, the attractiveness of oral acids contingent on concentration. Many carboxylic are attractive at ecologically relevant concentrations but become aversive beyond some maximal Recent work found that Drosophila ionotropic receptors IR25a and IR76b expressed sweet-responsive gustatory receptor neurons (GRNs) in labellum, a peripheral organ, mediate feeding behaviors toward dilute acids. Here, we disclose existence pharyngeal sensors D. melanogaster detect ingested also involved responses These rely IR51b, IR94a, IR94h, together with IR76b, drive We then demonstrate optogenetic activation either Ir94a + or Ir94h GRNs promotes an response, confirming their contributions behavior. Our discovery internal sour taste opens up new avenues for investigating sensation tastants insects.

Language: Английский

Citations

0
Pharyngeal neuronal mechanisms governing sour taste perception in Drosophila melanogaster DOI Creative Commons
Bhanu Shrestha, Jiun Sang, Suman Rimal

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Dec. 11, 2024

Sour taste, which is elicited by low pH, may serve to help animals distinguish appetitive from potentially harmful food sources. In all species studied date, the attractiveness of oral acids contingent on concentration. Many carboxylic are attractive at ecologically relevant concentrations but become aversive beyond some maximal Recent work found that Drosophila ionotropic receptors IR25a and IR76b expressed sweet-responsive gustatory receptor neurons (GRNs) in labellum, a peripheral organ, mediate feeding behaviors toward dilute acids. Here, we disclose existence pharyngeal sensors melanogaster detect ingested also involved responses These rely IR51b, IR94a, IR94h, together with IR76b, drive We then demonstrate optogenetic activation either Ir94a + or Ir94h GRNs promotes an response, confirming their contributions behavior. Our discovery internal sour taste opens up new avenues for investigating sensation tastants insects.

Language: Английский

Citations

0
Pharyngeal neuronal mechanisms governing sour taste perception inDrosophila melanogaster DOI Creative Commons
Bhanu Shrestha, Jiun Sang, Suman Rimal

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 30, 2024

Abstract Sour taste, which is elicited by low pH, may serve to help animals distinguish appetitive from potentially harmful food sources. In all species studied date, the attractiveness of oral acids contingent on concentration. Many carboxylic are attractive at ecologically relevant concentrations but become aversive beyond some maximal Recent work found that Drosophila ionotropic receptors IR25a and IR76b expressed sweet-responsive gustatory receptor neurons (GRNs) in labellum, a peripheral organ, mediate feeding behaviors toward dilute acids. Here, we disclose existence pharyngeal sensors D. melanogaster detect ingested also involved responses These rely IR51b, IR94a, IR94h, together with IR76b, drive We then demonstrate optogenetic activation either Ir94a + or Ir94h GRNs promotes an response, confirming their contributions behavior. Our discovery internal sour taste opens up new avenues for investigating sensation tastants insects.

Language: Английский

Citations

0
Experimental Evolution Induced by Maternal Post-copulatory Factors in Drosophila DOI

Ferveur Jean-François,

Sanjenbam Pratibha,

Fraichard Stéphane

et al.

Behavior Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Language: Английский

Citations

0
A single pair of pharyngeal neurons functions as a commander to reject high salt inDrosophila melanogaster DOI Creative Commons
Jiun Sang, Subash Dhakal, Bhanu Shrestha

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 17, 2023

Abstract Salt is an essential nutrient for survival, while excessive NaCl can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in pharynx are critical gatekeepers impacting decision to accept or reject a food. Currently, our understanding of mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high salt rudimentary. Here, we found that member ionotropic family, Ir60b expressed exclusively pair GRNs activated by salt. Using two-way choice assay (DrosoX) measure ingestion volume, demonstrate IR60b and two coreceptors IR25a IR76b, required prevent consumption. Mutants lacking external but retaining exhibit much higher avoidance than flies with all missing three IRs. Our findings highlight vital role IRs GRN control

Language: Английский

Citations

0