A neurogenetic toolkit to decodeAnopheles gambiaeolfaction DOI Open Access
Diego Giraldo, Andrew Hammond,

Wu Jinling

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 17, 2023

Summary The African malaria mosquito Anopheles gambiae exhibits a strong innate sensory drive to seek out human scent. To detect odorants, An. uses olfactory neurons (OSNs) that can be divided into different classes by unique repertoires of chemoreceptor gene expression. We applied CRISPR-Cas9-mediated T2A-In Frame Fusions and the QF2/QUAS system gain genetic access specific OSN subsets in expressing genes Ir25a , Ir76b Gr22 orco . first optimized methods generate cell-type QF2 driver QUAS responder lines map expression patterns these chemoreceptors across appendages. next transcuticular calcium imaging record neurophysiological responses select human-related odorants for each class. This neurogenetic toolkit tiling including those responsive CO 2 stands support systematic efforts decode olfaction this prolific disease vector at high-resolution combat malaria.

Language: Английский

Cross-modal sensory compensation increases mosquito attraction to humans DOI Creative Commons
Takeshi Morita, Nia G. Lyn, Ricarda K. von Heynitz

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 1, 2025

Sensory compensation occurs when loss of one sense leads to enhanced perception by another sense. We have identified a previously undescribed mechanism sensory in female Aedes aegypti mosquitoes. Odorant receptor co-receptor ( Orco ) mutants show attraction human skin temperature and increased heat-evoked neuronal activity foreleg neurons. Ir140 , foreleg-enriched member the ionotropic (IR) superfamily receptors, is up-regulated mutant legs. double do not heat seeking seen single mutants, suggesting that up-regulation key underlying mutants. Because expression sparse legs, this requires an indirect, long-range mechanism. Our findings highlight how mosquitoes, despite suffering olfactory loss, maintain overall effectiveness their host-seeking behavior up-regulating temperature, further enhancing status as most dangerous predator humans.

Language: Английский

Citations

3
Mosquito Cell Atlas: A single-nucleus transcriptomic atlas of the adultAedes aegyptimosquito DOI Creative Commons
Olivia V. Goldman,

Alexandra E. DeFoe,

Yanyan Qi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues adult male Aedes aegypti , providing cellular-level resolution mosquito We identify novel cell types expand our understanding sensory neuron organization chemoreceptors all tissues. Our analysis uncovers male-specific cells sexually dimorphic gene expression in antenna brain. In mosquitoes, find that glial brain, rather neurons, undergo most extensive transcriptional changes following blood feeding. findings provide insights into cellular basis behavior sexual dimorphism. MCA aims serve as resource for vector biology community, enabling systematic investigation cell-type specific across

Language: Английский

Citations

1
The auditory efferent system in mosquitoes DOI Creative Commons

YuMin M. Loh,

Matthew P. Su, David Ellis

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 27, 2023

Whilst acoustic communication forms an integral component of the mating behavior many insect species, it is particularly crucial for disease-transmitting mosquitoes; swarming males rely on hearing faint sounds flying females courtship initiation. That can hear within din a swarm testament to their fabulous auditory systems. Mosquito highly frequency-selective, remarkably sensitive and, most strikingly, supported by elaborate system efferent neurons that modulate function - only documented example amongst insects. Peripheral release octopamine, serotonin and GABA appears differentially across major disease-carrying mosquito with receptors from other neurotransmitter families also identified in ears. Because relies flight tones partners, offers new potential targets control. It represents unique model studying networks. Here we review current knowledge system, briefly compare its counterparts species highlight future research directions unravel contribution perception.

Language: Английский

Citations

17
A spatial map of antennal-expressed ionotropic receptors in the malaria mosquito DOI Creative Commons
Joshua I. Raji, Joanna K. Konopka, Christopher J. Potter

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(2), P. 112101 - 112101

Published: Feb. 1, 2023

The mosquito's antenna represents its main olfactory appendage for detecting volatile chemical cues from the environment. Whole-mount fluorescence in situ hybridization of ionotropic receptors (IRs) expressed antennae reveals that might be divisible into proximal and distal functional domains. number IR-positive cells appear stereotyped within each antennal segment (flagellomere). Highly odor-tuning IRs exhibit distinct co-localization patterns with IR coreceptors Ir8a, Ir25a, Ir76b predict their properties. Genetic knockin vivo imaging IR41c-expressing neurons indicate both odor-induced activation inhibition response to select amine compounds. Targeted mutagenesis IR41c does not abolish behavioral responses Our study provides a comprehensive map IR-expressing mosquitoes. These findings show organizing principles Anopheles neurons, which underlie contribution detection behaviorally relevant odors.

Language: Английский

Citations

12
The conserved IR75 subfamily mediates carboxylic acid detection in insects of public health and agricultural importance DOI Creative Commons

Matthew Cooke,

Michael Solomon Chembars,

R. Jason Pitts

et al.

Journal of Insect Science, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 1, 2025

Insects perceive and respond to carboxylic acids (CAs), amines, aldehydes primarily via conserved ionotropic receptors (IRs). These form the basis for a second olfactory system distinct from well-characterized odorant receptors. Neurons expressing IRs are housed in dedicated sensilla innervate glomeruli, separate those innervated by receptor neurons. The IR8a co-receptor is highly across insect orders and, together with tuning receptors, detects CAs. conservation of genes anatomical separation neural pathways underscore importance these compound classes CAs, specifically chemical ecology. We provide summary acid detection insects, focusing on dipteran lepidopteran species significance public health agriculture. An overview behavior toward CAs provided, as well comprehensive update function insects. Phylogenetic analysis publicly available genome databases reveals several that encode express homologs previously deorphanized highlighting avenues future research.

Language: Английский

Citations

0
An expanded neurogenetic toolkit to decode olfaction in the African malaria mosquito Anopheles gambiae DOI Creative Commons
Diego Giraldo, Andrew Hammond,

Wu Jinling

et al.

Cell Reports Methods, Journal Year: 2024, Volume and Issue: 4(2), P. 100714 - 100714

Published: Feb. 1, 2024

Anopheles gambiae uses its sense of smell to hunt humans. We report a two-step method yielding cell-type-specific driver lines for enhanced neuroanatomical and functional studies olfactory system. first integrated driver-responder-marker (DRM) system cassette consisting linked T2A-QF2 driver, QUAS-GFP responder, gut-specific transgenesis marker into four chemoreceptor genes (Ir25a, Ir76b, Gr22, orco) using CRISPR-Cas9-mediated homology-directed repair. The DRM facilitated rapid selection in-frame integrations via screening GFP+ sensory neurons (OSNs) in G

Language: Английский

Citations

2
An update and review of arthropod vector sensory systems: Potential targets for behavioural manipulation by parasites and other disease agents DOI
Jean‐François Doherty, Tahnee Ames, Leisl Imani Brewster

et al.

Advances in Parasitology/Advances in parasitology, Journal Year: 2024, Volume and Issue: unknown, P. 57 - 89

Published: Jan. 1, 2024

Language: Английский

Citations

1
Exploring new dimensions of immune cell biology in Anopheles gambiae through genetic immunophenotyping DOI Creative Commons
George-Rafael Samantsidis, Ryan C. Smith

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Abstract Mosquito immune cells, or hemocytes, are integral components of the innate responses that define vector competence. However, lack genetic resources has limited their characterization and our understanding functional roles in signaling. To overcome these challenges, we engineered transgenic Anopheles gambiae express fluorescent proteins under control candidate hemocyte promoters. Following five constructs through gene expression microscopy-based approaches, examine mosquito cell populations by leveraging advanced spectral imaging flow cytometry. Our results comprehensively map composition classifying them into twelve distinct based on size, granularity, ploidy, phagocytic capacity, PPO6, SPARC, LRIM15 markers. Together, novel use morphological properties markers provides increased resolution highlighting complexity plasticity populations, while providing foundation for deeper investigations immunity pathogen transmission.

Language: Английский

Citations

1
Livestock-Vector interaction using Volatile Organic Metabolites DOI
Merid N. Getahun

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: unknown, P. 101269 - 101269

Published: Sept. 1, 2024

Language: Английский

Citations

0
Development of a piggyBac transgenic system in Bactrocera dorsalis and its potential for research on olfactory molecular targets DOI Creative Commons
Jie Zhang, Qi Wang,

Jinxi Yuan

et al.

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Chemicals that modify pest behavior are developed to reduce crop damage by altering behavior, using specific genes within the olfactory system as molecular targets. The identification of these targets in Bactrocera dorsalis, also known functional study key genes, relies on CRISPR/Cas9-mediated gene knockout techniques. However, techniques face limitations when applied lethal genes. Transgenic technology offers a solution since it enables precise manipulation expression tissues or during certain developmental stages. Consequently, this piggyBac-mediated transgenic B. dorsalis investigate reporter organs, and assessed antennal electrophysiological responses lines. goal was assess potential approach for future research function. A universally expressed housekeeping from BdorActin family identified transcriptome dataset. Its candidate promoter region (BdorActinA3a-1P-2k) then cloned into piggyBac plasmid. We subsequently established two stable lines with TTAA insertion sites chromosomes 4 5, consistent characteristics transposition. strains exhibited essentially normal survival, hatchability adult lifespan unaffected, although there were slight reductions emergence rate oviposition capacity. fluorescent has been successfully olfactory-related such antennae, proboscis, maxillary palp, legs, external genitalia, brain. representative chemicals those wild type. some behaviors, pheromone response mating, significantly affected These findings suggest our could potentially be research, driving exogenous elements effective organs. caution is advised regarding its impact behavioral phenotypes.

Language: Английский

Citations

0