het-Ballorecognition inPodospora anserinais determined by pseudo-allelic interaction of genes encoding a HET and lectin fold domain protein and a PII-like protein DOI Creative Commons
Corinne Clavé,

Sonia Dheur,

S. Lorena Ament‐Velásquez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 19, 2023

Abstract Filamentous fungi display allorecognition genes that trigger regulated cell death (RCD) when strains of unlike genotype fuse. Podospora anserina is one several model species for the study this process termed heterokaryon or vegetative incompatibility. Incompatibility restricts transmission mycoviruses between isolates. In P. , genetic analyses have identified nine incompatibility loci, het loci. Here we set out to clone controlling het-B displays two incompatible alleles, het-B1 and het-B2 . We find locus encompasses adjacent genes, Bh Bp exist as highly divergent allelic variants ( Bh1/Bh2 Bp1/Bp2 ) in haplotypes. encodes a protein with an N-terminal HET domain, inducing domain bearing homology Toll/interleukin-1 receptor (TIR) domains C-terminal predicted lectin fold. The product homologous PII-like proteins, family small trimeric proteins acting sensors adenine nucleotides bacteria. show although system appears genetically allelic, fact determined by non-allelic Bh1/Bp2 interaction while reciprocal Bh2/Bp1 plays no role fold BH determines recognition specificity. Population studies genome indicate under balancing selection trans-species polymorphism, highlighting evolutionary significance addition emphasizing anew central TIR-like fungal RCD, identifies novel players completes characterization entire gene species. Author summary Many cellular life forms systems protect individuality discriminate conspecific self from non-self. filamentous fungi, fusion events are check specific upon detection restrict mycovirus parasitism. good form allorecognition, harbors Previous revealed these can be immune functions other phyla including bacteria, plants animals. cloned het-B, last remained found it complex comprising (related TIR domains) BP known bacterial metabolite sensors. Intriguingly, despite apparent allelism, dictated Bh/Bp interaction. This stresses reoccurring involvement RCD signs completion loci species, enabling comparative analysis different architectures underlying allorecognition.

Language: Английский

TIR signaling activates caspase-like immunity in bacteria DOI Creative Commons
François Rousset, Ilya А. Osterman, Tali Scherf

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Abstract Proteases of the caspase family, as well Toll/Interleukin-1 Receptor (TIR)-domain proteins, have central roles in innate immunity and regulated cell death humans. In this study we describe a bacterial immune system comprising both caspase-like protease TIR-domain protein. We found that TIR protein, once it recognizes phage invasion, produces previously unknown signaling molecule ADP-cyclo[N7:1′′]-ribose (N7-cADPR). This specifically activates which then indiscriminately degrades cellular proteins to halt replication. The TIR-caspase defense system, denote type IV Thoeris, is abundant bacteria efficiently protects against propagation. Our highlights diversity TIR-produced molecules demonstrates by proteases family an ancient mechanism immunity.

Language: Английский

Citations

0
Sexual reproduction is controlled by successive transcriptomic waves inPodospora anserina DOI Open Access
Frédérique Bidard, Pierre Grognet, Gaëlle Lelandais

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abstract Despite the inherent challenge of finding suitable mating partners, most eukaryotes use sexual reproduction to produce offspring endowed with increase genetic diversity and fitness. The persistence this mode is a key question in evolutionary biology. Fungi offer valuable insights into question, due their short lifecycle. This study focuses on Podospora anserina , pseudohomothallic ascomycete fungus that bypasses self-sterility by maintaining two compatible nuclei one mycelium. We performed genome-wide gene expression profiling during ten stages P. identified five major patterns. Our expert annotation approaches differentially expressed genes related secondary metabolite production, fungal vegetative incompatibility, programmed cell death, epigenetic regulation. In addition, master transcriptional regulators target networks were uncovered. provides comprehensive database for future functional genomics experiments novel pathway characterization reproduction.

Language: Английский

Citations

0
het-Ballorecognition inPodospora anserinais determined by pseudo-allelic interaction of genes encoding a HET and lectin fold domain protein and a PII-like protein DOI Creative Commons
Corinne Clavé,

Sonia Dheur,

S. Lorena Ament‐Velásquez

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 19, 2023

Abstract Filamentous fungi display allorecognition genes that trigger regulated cell death (RCD) when strains of unlike genotype fuse. Podospora anserina is one several model species for the study this process termed heterokaryon or vegetative incompatibility. Incompatibility restricts transmission mycoviruses between isolates. In P. , genetic analyses have identified nine incompatibility loci, het loci. Here we set out to clone controlling het-B displays two incompatible alleles, het-B1 and het-B2 . We find locus encompasses adjacent genes, Bh Bp exist as highly divergent allelic variants ( Bh1/Bh2 Bp1/Bp2 ) in haplotypes. encodes a protein with an N-terminal HET domain, inducing domain bearing homology Toll/interleukin-1 receptor (TIR) domains C-terminal predicted lectin fold. The product homologous PII-like proteins, family small trimeric proteins acting sensors adenine nucleotides bacteria. show although system appears genetically allelic, fact determined by non-allelic Bh1/Bp2 interaction while reciprocal Bh2/Bp1 plays no role fold BH determines recognition specificity. Population studies genome indicate under balancing selection trans-species polymorphism, highlighting evolutionary significance addition emphasizing anew central TIR-like fungal RCD, identifies novel players completes characterization entire gene species. Author summary Many cellular life forms systems protect individuality discriminate conspecific self from non-self. filamentous fungi, fusion events are check specific upon detection restrict mycovirus parasitism. good form allorecognition, harbors Previous revealed these can be immune functions other phyla including bacteria, plants animals. cloned het-B, last remained found it complex comprising (related TIR domains) BP known bacterial metabolite sensors. Intriguingly, despite apparent allelism, dictated Bh/Bp interaction. This stresses reoccurring involvement RCD signs completion loci species, enabling comparative analysis different architectures underlying allorecognition.

Language: Английский

Citations

0