Recent Advances in Biomolecule‐Engineered Metal‐Organic Frameworks (Bio‐MOFs): From Design, Bioengineering, and Structural/functional Regulation to Biocatalytic Applications

The Chemical Record, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Biomolecule‐engineered metal‐organic frameworks (Bio‐MOFs) are designed by incorporating biomolecules into or onto MOFs through covalent and non‐covalent interactions. These composite exhibit unique catalytic biological activities, making them highly suitable for various biocatalytic applications. In this review, we highlight recent advances in the material design, bioengineering methods, structural functional regulation techniques, applications of Bio‐MOFs. From a materials perspective, explore their structures multifunctional properties, including high surface area, tunable pore sizes, excellent biocompatibility. We also discuss techniques such as biomineralization post‐synthetic modification that employed synthesis. Furthermore, examine regulations Bio‐MOFs, which enhance activity stability interactions with enzymes, peptides, other biomolecules. Finally, analyze diverse reactions, biosensors/sensors, drug delivery, therapy, organic wastewater purification, emerging bio‐energy science. This review underscores pivotal role enhancing functions aims to inspire design synthesis novel Bio‐MOFs future bio‐related

Language: Английский

Enzymatic Synthesis of Unprotected α,β-Diamino Acids via Direct Asymmetric Mannich Reactions

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(29), P. 20263 - 20269

Published: July 13, 2024

α,β-Diamino acids are important structural motifs and building blocks for numerous bioactive natural products, peptidomimetics, pharmaceuticals, yet efficient asymmetric synthesis to access these stereoarrays remains a challenge. Herein, we report the development of pyridoxal 5'-phosphate (PLP)-dependent enzyme that is engineered catalyze stereoselective Mannich-type reactions between free α-amino enolizable cyclic imines. This biocatalyst enabled one-step enzymatic unusual pyrrolidine-containing amino acid L-tambroline at gram-scale with high enantio- diastereocontrol. Furthermore, this platform capable utilizing diverse range as Mannich donor various imines acceptor. By coupling different imine-generating enzymes, established versatile biocatalytic cascades demonstrated general, concise, versatile, atom-economic approach unprotected α,β-diamino acids, including structurally complex α,α-disubstituted contiguous stereocenters.

Language: Английский

Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion

iScience, Journal Year: 2023, Volume and Issue: 26(12), P. 108452 - 108452

Published: Nov. 17, 2023

We present a significant finding that Cu(II) ions can activate hematin (Hem) to generate more HOO⋅ and O2⋅- radicals from the decomposition of H2O2. Galactose oxidase (GO) have been simultaneously immobilized by coordinating (GO&Hem@Cu(II)). The dioxygen O2 be in situ generated byproduct H2O2 Cu(II)-activated hematin. Ample experimental evidence supports discovery GO is reactivated O2⋅-. For conversion 100 mM 5-hydroxymethylfurfural (HMF) water, GO&Hem@Cu(II) (0.8 mg/mL encapsulated) has achieved 99.5% within 180 min. In contrast, 0.8 free M3-5 variant (ACS Catalysis 2018, 8, 4025) an HMF 17.3%. (1,000 mM) (4 encapsulated), 98.8% after 8 h reaction.

Language: Английский