Virology Journal, Journal Year: 2023, Volume and Issue: 20(1)
Published: Nov. 27, 2023
Language: Английский
Viruses, Journal Year: 2022, Volume and Issue: 14(12), P. 2770 - 2770
Published: Dec. 12, 2022
There is mounting evidence of SARS-CoV-2 spillover from humans into many domestic, companion, and wild animal species. Research indicates that have infected white-tailed deer, deer-to-deer transmission has occurred, indicating deer could be a wildlife reservoir source novel variants. We examined the hypothesis Omicron variant actively asymptomatically infecting free-ranging New York City. Between December 2021 February 2022, 155 on Staten Island, York, were anesthetized for gross abnormalities illnesses. Paired nasopharyngeal swabs blood samples collected analyzed presence RNA antibodies. Of 135 serum samples, 19 (14.1%) indicated exposure, 11 reacted most strongly to wild-type B.1 lineage. 71 swabs, 8 positive (4 4 Delta). Two animals had active infections robust neutralizing antibodies, revealing reinfection or early seroconversion in deer. Variants concern continue circulate among may reinfect US populations, establish enzootic cycles wild: this warrants coordinated One Health response, proactively surveil, identify, curtail variants before they can spill back humans.
Language: Английский
Citations
31
Heliyon, Journal Year: 2024, Volume and Issue: 10(12), P. e33040 - e33040
Published: June 1, 2024
There has been considerable emphasis recently on the zoonotic origins of emerging infectious diseases in humans, including SARS-CoV-2 pandemic; however, reverse zoonoses (infections transmitted from humans to other animals) have received less attention despite their potential importance. The effects can be devastating for infected species and also result transmission pathogen back human populations or animals either original form as a variant. Humans animals, virus is able circulate evolve those species. As global travel resumes, zoonosis threatens endangered Nonhuman primates are particular concern given susceptibility respiratory infections. Enforcing safety measures all people working visiting wildlife areas, especially with nonhuman primates, increasing access living near protected areas that home will help mitigate transmission.
Language: Английский
Citations
5
Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 11
Published: Jan. 3, 2025
Widespread surveillance for SARS-CoV-2 was conducted across wildlife, captive animals in zoological collections, and domestic cats Nebraska from 2021 to 2023. The goal of this effort determine the prevalence, phylogenetic spatial distribution characteristics circulating variants using various diagnostic methodologies that can utilize both antemortem postmortem samples, which may be required wildlife such as white-tailed deer. Statewide testing revealed high variation prevalence among species, with deer identified primary reservoir. In 2021, seroprevalence 63.73% (n = 91) 39.66% 237) 2022, while virus detection retropharyngeal lymph nodes (RLN) 16.35% 483) 3.61% 277) 2022. Phylogenetic analysis on 11 positive samples 2021. This presence four lineages Delta variant: AY.100, AY.119, AY.3, AY.46.4. Conversely, other species showed no detection, except cats, had a low 2.38% 628) indicating minimal exposure. identification multiple underscores need ongoing importance different methodologies. These efforts are critical understanding circulation evolution animals, informing public health strategies, mitigating risks zoonotic transmission emerging infectious diseases.
Language: Английский
Citations
0
Emerging infectious diseases, Journal Year: 2025, Volume and Issue: 31(2)
Published: Jan. 10, 2025
White-tailed deer (Odocoileus virginianus) have high value for research, conservation, agriculture, and recreation might be key SARS-CoV-2 reservoirs. In November 2023, we sampled 15 female in a captive facility Texas, USA. All had neutralizing antibodies to SARS-CoV-2; respiratory swab samples from 11 were SARS-CoV-2-positive by quantitative reverse transcription PCR, 1 also positive rectal sample. Six of the yielded infectious virus; replication kinetics most displayed lower growth 24-48 hours postinfection vitro than Omicron lineages isolated humans Texas same period. Virus was similar between groups 72 hours, suggesting no strong attenuation deer-derived virus. viruses clustered XBB clade demonstrated more mutations expected compared with contemporaneous humans, that crossing species barrier accompanied substitution rate.
Language: Английский
Citations
0
Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 600 - 600
Published: March 5, 2025
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) continues to pose a significant threat public health. Notably, SARS-CoV-2 demonstrates the capacity infect various non-human animal species, including both captive and free-living animals. Earlier experimental studies revealed low susceptibility of domestic cattle (Bos taurus) ancestral B.1 lineage; however, recent findings indicate greater permissiveness Delta variant. While some detected evidence infection in Italy, Germany, India, Nigeria, currently, there is no infections US cattle. We have investigated over 600 samples, pre-pandemic pandemic sera collected from Pennsylvania for presence antibodies. Since serological tests inherent problems false positives negatives, we conducted comprehensive assessment multiple assays. As are known positive serum used hyperimmune raised with SARS-CoV-2-spike receptor binding domain (RBD) as control test validation. found that pseudovirus neutralization assays luciferase reporter system can produce results, care must be taken interpret diagnosis using these natural or transmission among US. This study underscores importance robust evaluation when employing detection populations.
Language: Английский
Citations
0
Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 507 - 507
Published: March 31, 2025
During the COVID-19 pandemic, severe acute respiratory syndrome coronavirus 2 (SC2) infection was confirmed in various animal species demonstrating a wide host range of virus. Prior studies have shown that ACE2 protein is primary receptor used by virus to gain cellular entry and begin replication cycle. In previous studies, we demonstrated human bat proteins can be utilized SC2 viruses for entry. Bats are suspected natural because genetic homology with other coronaviruses. this work, demonstrate expression genes from common vampire (CVB) (Desmodus rotundus) pallid (PB) (Antrozous pallidus), supports some cell culture. Two lines were produced, CVB-ACE2 PB-ACE2, expressing these along TMPRSS2, model previously established using non-permissive chicken DF-1 line. Results original Wuhan lineage (WA1) Delta variant able infect replicate either lines. contrast, Lambda Omicron infected both lines, but viral titers did not increase following infection. Viral detection immunofluorescence abundant spike (S) staining WA1 variants little signal variants. These while CVB PB infection, later (Lambda Omicron) poorly observations suggest more efficient adaption become less fit species.
Language: Английский
Citations
0
Microorganisms, Journal Year: 2022, Volume and Issue: 10(11), P. 2166 - 2166
Published: Oct. 31, 2022
In the midst of a persistent pandemic probable zoonotic origin, one needs to constantly evaluate interplay SARS-CoV-2 (severe acute respiratory syndrome-related coronavirus-2) with animal populations. Animals can get infected from humans, and certain species, including mink white-tailed deer, exhibit considerable animal-to-animal transmission resulting in potential endemicity, mutation pressure, possible secondary spillover humans. We attempt comprehensive review available data on species by SARS-CoV-2, as presented scientific literature official reports relevant organizations. further lessons humans should learn outbreaks, deer zoo threat for conservation, implication rodents evolution novel variants such Omicron, role pets reservoirs virus. Finally, we outline need broader approach epidemics, general, incorporating principles One Health Planetary Health.
Language: Английский
Citations
18
Viruses, Journal Year: 2022, Volume and Issue: 14(10), P. 2290 - 2290
Published: Oct. 18, 2022
Several studies reported a high prevalence of SARS-CoV-2 among white-tailed deer in North America. Monitoring cervids all regions to better understand infection and circulation other populations has been urged. To evaluate exposure and/or to/by Poland, we sampled 90 red shot by hunters five hunting districts north-eastern Poland. Serum nasopharyngeal swabs were collected, then an immunofluorescent assay (IFA) detect anti-SARS-CoV-2 antibodies was performed as well real-time PCR with reverse transcription for direct virus detection. No positive samples detected. There is no evidence spillover from the human population
Language: Английский
Citations
12
British Journal of Clinical Pharmacology, Journal Year: 2024, Volume and Issue: 90(9), P. 2137 - 2158
Published: May 31, 2024
Aim Understanding how COVID‐19 impacts the expression of clinically relevant drug metabolizing enzymes and membrane transporters (DMETs) is vital for addressing potential safety efficacy concerns related to systemic peripheral concentrations. This study investigates impact severity on DMETs underlying mechanisms inform design precise clinical dosing regimens affected patients. Methods Transcriptomics analysis 102 DMETs, 10 inflammatory markers, 12 xenosensing regulatory genes was conducted nasopharyngeal swabs from 50 SARS‐CoV‐2 positive (17 outpatients, 16 non‐ICU, 17 ICU) 13 negative individuals, tested through qPCR, in Greater Toronto area October 2020 2021. Results We observed a significant differential gene 42 6 9 genes. associated with upregulation AKR1C1 , MGST1 SULT1E1 downregulation ABCC10 CYP3A43 SLC29A4 expressions. Altogether, SARS‐CoV‐2‐positive patients showed an CYP2C9 CYP2C19 SULT1B1 SULT2B1 SLCO4A1 FMO5 MGST3 ABCC5 SLCO4C1 compared individuals. These dysregulations were changes driven by disease. GSTM3 PPARA are biomarkers dysregulation pattern ICU patients, respectively. Conclusion The involved processing commonly prescribed drugs, suggesting disease–drug interactions, especially narrow therapeutic index drugs.
Language: Английский
Citations
2
Animals, Journal Year: 2023, Volume and Issue: 13(16), P. 2554 - 2554
Published: Aug. 8, 2023
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in humans late 2019 and spread rapidly, becoming a global pandemic. A zoonotic spillover event from animal to human was identified as the presumed origin. Subsequently, reports began emerging regarding spillback events resulting SARS-CoV-2 infections multiple species. These highlighted critical links between health while also raising concerns about development of new reservoir hosts potential viral mutations that could alter virulence transmission or evade immune responses. Characterizing susceptibility, prevalence, species became priority help protect health. In this study, we coalesced large team investigators community partners surveil for domestic free-ranging animals around Ohio May 2020 August 2021. We focused on with known predicted susceptibility infection, highly congregated medically compromised (e.g., shelters, barns, veterinary hospitals), had frequent contact pets, agricultural animals, zoo wildlife hospitals). This included deer (
Language: Английский
Citations
6