Nuclear Structure, Size Regulation, and Role in Cell Migration DOI Creative Commons
Yuhao Li,

Shanghao Ge,

Jiayi Liu

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2130 - 2130

Published: Dec. 23, 2024

The nucleus serves as a pivotal regulatory and control hub in the cell, governing numerous aspects of cellular functions, including DNA replication, transcription, RNA processing. Therefore, any deviations nuclear morphology, structure, or organization can strongly affect activities. In this review, we provide an updated perspective on structure function components, focusing linker nucleoskeleton cytoskeleton complex, envelope, lamina, chromatin. Additionally, size should be considered fundamental parameter for state. Its regulation is tightly linked to environmental changes, development, various diseases, cancer. Hence, also concise overview different mechanisms by which determined, emerging role mechanical sensor, implications altered morphology physiology diseased cells.

Language: Английский

A novel SUN1-ALLAN complex coordinates segregation of the bipartite MTOC across the nuclear envelope during rapid closed mitosis in Plasmodium DOI Open Access
Mohammad Zeeshan, Igor Blatov, Ryuji Yanase

et al.

Published: April 10, 2025

Mitosis in eukaryotes involves reorganisation of the nuclear envelope (NE) and microtubule-organising centres (MTOCs). During male gametogenesis Plasmodium, causative agent malaria, mitosis is exceptionally rapid highly divergent. Within 8 min, haploid gametocyte genome undergoes three replication cycles (1N to 8N), while maintaining an intact NE. Axonemes assemble cytoplasm connect a bipartite MTOC-containing pole (NP) cytoplasmic basal body, producing eight flagellated gametes. The mechanisms coordinating NE remodelling, MTOC dynamics, flagellum assembly remain poorly understood. We identify SUN1-ALLAN complex as novel mediator remodelling coordination during Plasmodium berghei gametogenesis. SUN1, conserved protein, localises dynamic loops focal points at nucleoplasmic face spindle poles. ALLAN, divergent allantoicase, has location like that these proteins form unique complex, detected by live-cell imaging, ultrastructural expansion microscopy, interactomics. Deletion either SUN1 or ALLAN genes disrupts organisation, leading body mis-segregation, defective assembly, impaired microtubule-kinetochore attachment, but axoneme formation remains intact. Ultrastructural analysis revealed miscoordination, aberrant gametes lacking material. These defects block development mosquito parasite transmission, highlighting essential functions this complex.

Language: Английский

Citations

0
In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana DOI Creative Commons
Hugo Germain, Ingrid Berenice Sanchez Carrillo, Patrick C. Hoffmann

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract The nucleus is delimited by the nuclear envelope (NE), where pore complexes (NPCs) are embedded. While this complex has been well studied in vertebrates, yeast, and, more recently, algae, situ structural data of higher plants still missing. Here, we show that many individual nucleoporins Arabidopsis thaliana and human present high similarity. We report a first plant NPC structure, derived from A. root protoplasts using cryo electron tomography, subtomogram averaging homology-based integrative modeling. model based on predicted models NUPs identified mass spectrometry. scaffold exhibits differences diameter height to C. reinhardtii, while sharing some features with H. sapiens NPCs. Notably, observed contains NUP155 connectors like NPC, which may explain difference when compared reinhardtii NPC.

Language: Английский

Citations

0
A novel SUN1-ALLAN complex coordinates segregation of the bipartite MTOC across the nuclear envelope during rapid closed mitosis in Plasmodium DOI Open Access
Mohammad Zeeshan, Igor Blatov, Ryuji Yanase

et al.

Published: April 10, 2025

Mitosis in eukaryotes involves reorganization of the nuclear envelope (NE) and microtubule-organizing centres (MTOCs). During male gametogenesis Plasmodium, causative agent malaria, mitosis is exceptionally rapid highly divergent. Within 8 min, haploid gametocyte genome undergoes three replication cycles (1N to 8N), while maintaining an intact NE. Axonemes assemble cytoplasm connect a bipartite MTOC-containing pole (NP) cytoplasmic basal body, producing eight flagellated gametes. The mechanisms coordinating NE remodelling, MTOC dynamics, flagellum assembly remain poorly understood.We identify SUN1-ALLAN complex as novel mediator remodelling coordination during Plasmodium gametogenesis. SUN1, conserved protein, localizes dynamic loops focal points at nucleoplasmic face spindle poles. ALLAN, divergent allantoicase, has location like that these proteins form unique complex, detected by live-cell imaging, ultrastructural expansion microscopy, interactomics. Deletion either SUN1 or ALLAN genes disrupts organization, leading body mis-segregation, defective assembly, impaired microtubule-kinetochore attachment, but axoneme formation remains intact. Ultrastructural analysis revealed miscoordination, aberrant gametes lacking material. These defects block development mosquito parasite transmission, highlighting essential functions this complex.

Language: Английский

Citations

0
Function of nuclear envelope proteins in plant growth and development DOI Creative Commons

Olivia S Hazelwood,

Norman B. Best, M. Arif Ashraf

et al.

Current Opinion in Plant Biology, Journal Year: 2025, Volume and Issue: 85, P. 102738 - 102738

Published: May 16, 2025

Language: Английский

Citations

0
A novel SUN1-ALLAN complex coordinates segregation of the bipartite MTOC across the nuclear envelope during rapid closed mitosis in Plasmodium berghei DOI Creative Commons
Mohammad Zeeshan, Igor Blatov, Ryuji Yanase

et al.

eLife, Journal Year: 2025, Volume and Issue: 14

Published: May 20, 2025

Mitosis in eukaryotes involves reorganisation of the nuclear envelope (NE) and microtubule-organising centres (MTOCs). During male gametogenesis Plasmodium , causative agent malaria, mitosis is exceptionally rapid highly divergent. Within 8 min, haploid gametocyte genome undergoes three replication cycles (1N to 8N), while maintaining an intact NE. Axonemes assemble cytoplasm connect a bipartite MTOC-containing pole (NP) cytoplasmic basal body, producing eight flagellated gametes. The mechanisms coordinating NE remodelling, MTOC dynamics, flagellum assembly remain poorly understood. We identify SUN1-ALLAN complex as novel mediator remodelling coordination during berghei gametogenesis. SUN1, conserved protein, localises dynamic loops focal points at nucleoplasmic face spindle poles. ALLAN, divergent allantoicase, has location like that these proteins form unique complex, detected by live-cell imaging, ultrastructural expansion microscopy, interactomics. Deletion either SUN1 or ALLAN genes disrupts organisation, leading body mis-segregation, defective assembly, impaired microtubule-kinetochore attachment, but axoneme formation remains intact. Ultrastructural analysis revealed miscoordination, aberrant gametes lacking material. These defects block development mosquito parasite transmission, highlighting essential functions this complex.

Language: Английский

Citations

0
A novel SUN1-ALLAN complex coordinates segregation of the bipartite MTOC across the nuclear envelope during rapid closed mitosis in Plasmodium DOI Open Access
Mohammad Zeeshan, Igor Blatov, Ryuji Yanase

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

Mitosis in eukaryotes involves reorganization of the nuclear envelope (NE) and microtubule-organizing centres (MTOCs). During male gametogenesis Plasmodium, causative agent malaria, mitosis is exceptionally rapid highly divergent. Within 8 min, haploid gametocyte genome undergoes three replication cycles (1N to 8N), while maintaining an intact NE. Axonemes assemble cytoplasm connect a bipartite MTOC-containing pole (NP) cytoplasmic basal body, producing eight flagellated gametes. The mechanisms coordinating NE remodelling, MTOC dynamics, flagellum assembly remain poorly understood. We identify SUN1-ALLAN complex as novel mediator remodelling coordination during Plasmodium gametogenesis. SUN1, conserved protein, localizes dynamic loops focal points at nucleoplasmic face spindle poles. ALLAN, divergent allantoicase, has location like that these proteins form unique complex, detected by live-cell imaging, ultrastructural expansion microscopy, interactomics. Deletion either SUN1 or ALLAN genes disrupts organization, leading body mis-segregation, defective assembly, impaired microtubule-kinetochore attachment, but axoneme formation remains intact. Ultrastructural analysis revealed miscoordination, aberrant gametes lacking material. These defects block development mosquito parasite transmission, highlighting essential functions this complex.

Language: Английский

Citations

2
Nuclear pores beyond macromolecule channels DOI
Sachihiro Matsunaga, Nanami Ito

Nature Plants, Journal Year: 2024, Volume and Issue: 10(6), P. 842 - 843

Published: May 21, 2024

Language: Английский

Citations

0
Nuclear Structure, Size Regulation, and Role in Cell Migration DOI Creative Commons
Yuhao Li,

Shanghao Ge,

Jiayi Liu

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2130 - 2130

Published: Dec. 23, 2024

The nucleus serves as a pivotal regulatory and control hub in the cell, governing numerous aspects of cellular functions, including DNA replication, transcription, RNA processing. Therefore, any deviations nuclear morphology, structure, or organization can strongly affect activities. In this review, we provide an updated perspective on structure function components, focusing linker nucleoskeleton cytoskeleton complex, envelope, lamina, chromatin. Additionally, size should be considered fundamental parameter for state. Its regulation is tightly linked to environmental changes, development, various diseases, cancer. Hence, also concise overview different mechanisms by which determined, emerging role mechanical sensor, implications altered morphology physiology diseased cells.

Language: Английский

Citations

0