PubMed, Journal Year: 2024, Volume and Issue: 2024

Published: Jan. 1, 2024

Monitoring specific antibodies derived from whole-cell immunization through cell-based ELISA methods poses challenges due to humoral responses against various cell proteins. In this report, we outline a technique involving pre-adsorption on cells remove undesirable immune serum. This step provides the subsequent monitoring of targeted antigen using tANCHOR-based ELISA. Notably, approach accelerates result acquisition, eliminating necessity purify expressed or obtain customized peptide for coating assay plates.

Language: Английский