SOX7 inhibits the malignant progression of bladder cancer via the DNMT3B/CYGB axis

Molecular Biomedicine, Journal Year: 2024, Volume and Issue: 5(1)

Published: Sept. 4, 2024

Abstract Bladder cancer (BCa) stands out as a highly prevalent malignant tumor affecting the urinary system. The Sex determining region Y-box protein family is recognized for its crucial role in BCa progression. However, effect of 7 (SOX7) on progression has not been fully elucidated. Herein, RNA-sequencing, western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF) and tissue microarray were utilized to assess SOX7 expression vitro vivo. Additionally, expression, prognosis, + cytoglobin (CYGB) score analyzed using R software. In vivo experiments performed with cell lines validate knockdown overexpression BCa. results showed that exhibits low It functions diverse capacities, inhibiting proliferative, migratory, invasive capabilities addition, experimental database demonstrated binds promoter DNA methyltransferase 3 beta (DNMT3B), leading transcriptional inhibition DNMT3B. This subsequently reduced methylation CYGB promoter, ultimately scores significantly linked patient prognosis. conclusion, inhibits via DNMT3B/CYGB axis. capable predicting prognostic outcomes patients. Therefore, may play an important bladder cancer, they can be used markers

Language: Английский

Bladder cancer: non-coding RNAs and exosomal non-coding RNAs

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(5)

Published: Aug. 31, 2024

Language: Английский