Echinacoside inhibits hepatocellular carcinoma progression by targeting the miR-30c-5p/FOXD1/KLF12 axis DOI
Guoyu Wang, Yang Han,

Juhua Zhuang

et al.

Technology and Health Care, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 16

Published: Aug. 29, 2024

BACKGROUND: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-attributed mortality and primary liver malignancy in world. Echinacoside a phenylethanoid glycoside derived from traditional Chinese medicinal herbs which possessed multiple health benefits on humans, including anti-tumor effects. OBJECTIVE: This study aimed to demonstrate function echinacoside HCC progression involvement miR-30c-5p/FOXD1/KLF12 axis. METHODS: The HepG2 cells were treated by different dose echinacoside, miR-30c-5p mimic, inhibitor, FOXD1 overexpression lentiviruses or siRNA individually simultaneously. cell invasion migration measured transwell assay. RNA protein levels tested RT-PCR western blot, respectively. regulatory Forkhead box D1 (FOXD1), Krüppel-like factor 12 (KLF12) was luciferase reporter assay or/and ChIP Meanwhile, cancer lung metastasis mice model used examine functions vivo. Moreover, correlations among miR-30c-5p, FOXD1, KLF12, prognosis analyzed using clinical sample TCGA database. RESULTS: Based both vitro vivo investigations, we found that could inhibit migration, invasiveness, tumor metastasis, associated with enhanced Furthermore, through analyzing interactions intermediate molecules, revealed KLF12üere clinically relevant each other patients, correlated prognosis, regulated contribute inhibition progression. CONCLUSIONS: These findings suggest progression, mechanism related abovementioned molecules might serve as prospective biomarkers for prognosis.

Language: Английский

KLF12 inhibits lipopolysaccharide-induced inflammatory responses, oxidative stress, pyroptosis, and endoplasmic reticulum stress in human airway epithelial cells through inhibition of the NF-κB pathway DOI

Xiujuan Xu,

Yiping Yu

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2025, Volume and Issue: unknown, P. 119917 - 119917

Published: Feb. 1, 2025

Language: Английский

Citations

0
KLF12 transcriptional activation by a novel LncRNA A930015D03Rik enhances melanoma metastasis DOI
Meng Xiang, Ziyi Li, Jie Su

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 309, P. 142686 - 142686

Published: April 4, 2025

Language: Английский

Citations

0
The role of circular RNAs in regulating cytokine signaling in cancer DOI Creative Commons
Vandana Joshi,

Swati Swati,

Amit Mishra

et al.

FEBS Open Bio, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Dysregulation of cytokine signaling is central to the development and progression cancer. Cytokines are not only involved in promoting cancer but also regulate anti‐tumor immune responses. Circular RNAs (circRNAs) single‐stranded, covalently closed RNA molecules lacking free ends, which have emerged as critical regulators signaling. Transcriptional post‐transcriptional regulation by circRNAs contributes pathogenesis. Here, we discuss emerging role modulating pathways that development. In particular, examine TGF‐β, IL‐6, IL‐10, TNF‐α, VEGF, FGF, PDGF, chemokine

Language: Английский

Citations

0
Echinacoside inhibits hepatocellular carcinoma progression by targeting the miR-30c-5p/FOXD1/KLF12 axis DOI
Guoyu Wang, Yang Han,

Juhua Zhuang

et al.

Technology and Health Care, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 16

Published: Aug. 29, 2024

BACKGROUND: Hepatocellular carcinoma (HCC) is the third leading cause of cancer-attributed mortality and primary liver malignancy in world. Echinacoside a phenylethanoid glycoside derived from traditional Chinese medicinal herbs which possessed multiple health benefits on humans, including anti-tumor effects. OBJECTIVE: This study aimed to demonstrate function echinacoside HCC progression involvement miR-30c-5p/FOXD1/KLF12 axis. METHODS: The HepG2 cells were treated by different dose echinacoside, miR-30c-5p mimic, inhibitor, FOXD1 overexpression lentiviruses or siRNA individually simultaneously. cell invasion migration measured transwell assay. RNA protein levels tested RT-PCR western blot, respectively. regulatory Forkhead box D1 (FOXD1), Krüppel-like factor 12 (KLF12) was luciferase reporter assay or/and ChIP Meanwhile, cancer lung metastasis mice model used examine functions vivo. Moreover, correlations among miR-30c-5p, FOXD1, KLF12, prognosis analyzed using clinical sample TCGA database. RESULTS: Based both vitro vivo investigations, we found that could inhibit migration, invasiveness, tumor metastasis, associated with enhanced Furthermore, through analyzing interactions intermediate molecules, revealed KLF12üere clinically relevant each other patients, correlated prognosis, regulated contribute inhibition progression. CONCLUSIONS: These findings suggest progression, mechanism related abovementioned molecules might serve as prospective biomarkers for prognosis.

Language: Английский

Citations

0