Intestinal organoids: The path towards clinical application DOI Creative Commons

Hady Yuki Sugihara,

Ryuichi Okamoto,

Tomohiro Mizutani

et al.

European Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 104(1), P. 151474 - 151474

Published: Dec. 25, 2024

Organoids have revolutionized the whole field of biology with their ability to model complex three-dimensional human organs in vitro. Intestinal organoids were especially consequential as first successful long-term culture intestinal stem cells, which raised hopes for translational medical applications. Despite significant contributions basic research, challenges remain develop into clinical tools diagnosis, prognosis, and therapy. In this review, we outline current state research involving adult cell pluripotent derived organoids, highlighting advances limitations disease modeling, drug-screening, personalized medicine, Preclinical studies demonstrated a remarkable functional recapitulation infectious genetic diseases, there is mounting evidence reliability patient-specific avatar. Breakthroughs now allow generation structurally cellularly models better capture wider range pathophysiology. As develops evolves, need standardized frameworks generation, culture, storage, analysis ensure reproducibility, comparability, interpretability these preclinical ultimately enable translation.

Language: Английский

Inflamed intestinal epithelial cells from patients with ulcerative colitis restore a non-inflamed transcriptional profile upon in vitro expansion DOI Creative Commons
Alexander Hammerhøj, Theresa Louise Boye, Jiayi Yao

et al.

Laboratory Investigation, Journal Year: 2025, Volume and Issue: unknown, P. 104172 - 104172

Published: April 1, 2025

Language: Английский

Citations

0
Advances in the research of intestinal fungi in Crohn's disease DOI
Mo-Wei Kong, Yu Yang, Peng Wang

et al.

World Journal of Gastroenterology, Journal Year: 2024, Volume and Issue: 30(39), P. 4318 - 4323

Published: Oct. 11, 2024

This article reviews of the original research published by Wu et al in World Journal Gastroenterology , delving into pivotal role gut microbiota pathogenesis Crohn's disease (CD). Insights were gained from fecal transplantation (FMT) mouse models, revealing intricate interplay between microbiota, mesenteric adipose tissue (MAT), and creeping fat. The study uncovered characteristics inflammation fibrosis MAT intestinal tissues patients with CD; moreover, through FMT model, it observed impact samples healthy those CD on symptoms. is complex, its etiology remains unclear; however, widely believed that dysbiosis plays a significant role. Recently, development application next-generation sequencing technology, fungi chronicity has deepened. editorial serves as supplement to who discussed advances related CD.

Language: Английский

Citations

1
Dissecting Innate and Adaptive Immunity in Inflammatory Bowel Disease: Immune Compartmentalization, Microbiota Crosstalk, and Emerging Therapies DOI Creative Commons

Ningning Yue,

Peng Hu,

Cheng‐mei Tian

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 9987 - 10014

Published: Nov. 1, 2024

The intestinal immune system is the largest organ in human body. Excessive response to cavity induced by harmful stimuli including pathogens, foreign substances and food antigens an important cause of inflammatory diseases such as celiac disease bowel (IBD). Although great progress has been made treatment IBD some immune-related biotherapeutic products, yet a considerable proportion patients remain unresponsive or tolerant immunotherapeutic strategy. Therefore, it necessary further understand mechanism cell populations involved enteritis, dendritic cells, macrophages natural lymphocytes, steady-state tolerance IBD, order find effective therapy. In this review, we discussed role innate adaptive immunity development IBD. And relationship between disorders microflora crosstalk were also presented. We focus on new findings field T immunity, which might identify novel cytokines, chemokines anti-cytokine antibodies approaches for

Language: Английский

Citations

1
Establishment of an ulcerative colitis model using colon organoids derived from human induced pluripotent stem cells DOI Creative Commons

Fuki Yokoi,

Sayaka Deguchi, Yukio Watanabe

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(10), P. 111049 - 111049

Published: Sept. 27, 2024

Language: Английский

Citations

0
Intestinal organoids: The path towards clinical application DOI Creative Commons

Hady Yuki Sugihara,

Ryuichi Okamoto,

Tomohiro Mizutani

et al.

European Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 104(1), P. 151474 - 151474

Published: Dec. 25, 2024

Organoids have revolutionized the whole field of biology with their ability to model complex three-dimensional human organs in vitro. Intestinal organoids were especially consequential as first successful long-term culture intestinal stem cells, which raised hopes for translational medical applications. Despite significant contributions basic research, challenges remain develop into clinical tools diagnosis, prognosis, and therapy. In this review, we outline current state research involving adult cell pluripotent derived organoids, highlighting advances limitations disease modeling, drug-screening, personalized medicine, Preclinical studies demonstrated a remarkable functional recapitulation infectious genetic diseases, there is mounting evidence reliability patient-specific avatar. Breakthroughs now allow generation structurally cellularly models better capture wider range pathophysiology. As develops evolves, need standardized frameworks generation, culture, storage, analysis ensure reproducibility, comparability, interpretability these preclinical ultimately enable translation.

Language: Английский

Citations

0