Ultrasound-Triggered drug release and cytotoxicity of microbubbles with diverse drug Attributes DOI Creative Commons

Chi-Fen Chuang,

Chia-Wei Lin,

Chih‐Kuang Yeh

et al.

Ultrasonics Sonochemistry, Journal Year: 2024, Volume and Issue: 112, P. 107182 - 107182

Published: Dec. 1, 2024

Ultrasound (US)-triggered cavitation of drug-loaded microbubbles (MBs) represents a promising approach for targeted drug delivery, with substantial benefits attainable through precise control over release dosage and form. This study investigates Camptothecin-loaded MBs (CPT-MBs) Doxorubicin-loaded (DOX-MBs), focusing on how properties such as hydrophilicity, hydrophobicity, charged functional groups affect their interaction the lipid surfaces MBs, thereby influencing fundamental characteristics acoustic MBs. In comparison to DOX-MBs, CPT-MBs showed larger MB size (2.2 ± 0.3 1.4 0.1 μm, respectively), 2-fold increase in loading, an 18 % reduction leakage after 2 h at 37℃. Under 1 MHz US 100 ms pulse repetition interval (PRI), 1000 cycles, 5-minute duration, 550 kPa pressure, undergo inertial cavitation, while DOX-MBs stable cavitation. Drug particles released from these under US-induced were analyzed using dynamic light scattering, NanoSight, cryo-electron microscopy, density gradient ultracentrifugation. Results that mainly free CPT, multilayered DOX-lipid aggregates. The cytotoxicity C6 cells induced by US-triggered two types also differed. aggregates delayed initial uptake, leading less pronounced short-term (2 h) effects compared rapid CPT CPT-MBs. These findings underscore need optimize delivery strategies fine-tuning composition parameters kinetics achieve best tumoricidal outcomes.

Language: Английский

Emerging trends and hot topics in the application of multi-omics in drug discovery: A bibliometric and visualized study DOI Creative Commons
Ziheng Wang, Yang Zhao, Long Zhang

et al.

Current Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 21(1), P. 20 - 32

Published: Dec. 1, 2024

Language: Английский

Citations

5
Systems Biology of Dry Eye: Unraveling Molecular Mechanisms through Multi-Omics Integration DOI
Zhirui Zhang, Changxing Liu, Lingying Zhao

et al.

The Ocular Surface, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

1
Generative Models Validation via Manifold Recapitulation Analysis DOI Creative Commons
Nicolo’ Lazzaro,

Gianluca Leonardi,

Raffaele Marchesi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 26, 2024

Abstract Summary Single-cell transcriptomics increasingly relies on nonlinear models to harness the dimensionality and growing volume of data. However, most model validation focuses local manifold fidelity (e.g., Mean Squared Error other data likelihood metrics), with little attention global topology these should ideally be learning. To address this limitation, we have implemented a robust scoring pipeline aimed at validating model’s ability reproduce entire reference manifold. The Python library Cytobench demonstrates approach, along Jupyter Notebooks an example dataset help users get started workflow. Manifold recapitulation analysis can used develop assess intended learn full network cellular dynamics, as well validate their performance external datasets. Availability A implementing has been made available via pip inspected GitHub alongside some demonstrating its application. Contact [email protected] or [email protected]

Language: Английский

Citations

0
Ultrasound-Triggered drug release and cytotoxicity of microbubbles with diverse drug Attributes DOI Creative Commons

Chi-Fen Chuang,

Chia-Wei Lin,

Chih‐Kuang Yeh

et al.

Ultrasonics Sonochemistry, Journal Year: 2024, Volume and Issue: 112, P. 107182 - 107182

Published: Dec. 1, 2024

Ultrasound (US)-triggered cavitation of drug-loaded microbubbles (MBs) represents a promising approach for targeted drug delivery, with substantial benefits attainable through precise control over release dosage and form. This study investigates Camptothecin-loaded MBs (CPT-MBs) Doxorubicin-loaded (DOX-MBs), focusing on how properties such as hydrophilicity, hydrophobicity, charged functional groups affect their interaction the lipid surfaces MBs, thereby influencing fundamental characteristics acoustic MBs. In comparison to DOX-MBs, CPT-MBs showed larger MB size (2.2 ± 0.3 1.4 0.1 μm, respectively), 2-fold increase in loading, an 18 % reduction leakage after 2 h at 37℃. Under 1 MHz US 100 ms pulse repetition interval (PRI), 1000 cycles, 5-minute duration, 550 kPa pressure, undergo inertial cavitation, while DOX-MBs stable cavitation. Drug particles released from these under US-induced were analyzed using dynamic light scattering, NanoSight, cryo-electron microscopy, density gradient ultracentrifugation. Results that mainly free CPT, multilayered DOX-lipid aggregates. The cytotoxicity C6 cells induced by US-triggered two types also differed. aggregates delayed initial uptake, leading less pronounced short-term (2 h) effects compared rapid CPT CPT-MBs. These findings underscore need optimize delivery strategies fine-tuning composition parameters kinetics achieve best tumoricidal outcomes.

Language: Английский

Citations

0