Generation of bovine iPSCs from fetal fibroblasts for in vitro myogenesis and cultured meat DOI Creative Commons
Kaiana Recchia, Methi Wathikthinnakon, Fabiana Fernandes Bressan

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: May 16, 2025

Introduction Emerging biotechnologies are increasingly being explored for food production, including the development of cell-cultivated meat. Conventional approaches typically rely on satellite cell (SC) biopsies, which present challenges in scalability. Bovine induced pluripotent stem cells (biPSCs) represent a promising alternative due to their capacity self-renewal and developmental plasticity. Methods This study utilized both lentiviral (integrating) episomal (non-integrating) reprogramming strategies generate biPSCs suitable myogenic differentiation. fetal fibroblasts (bFFs) were reprogrammed using vectors pMaster K pCXB-EBNA1, leading emergence putative iPSC colonies 13 days post-nucleofection. A clonal line, bFF-iPSCs pMK, was selected further analysis. Results The pMK line expressed key pluripotency markers alkaline phosphatase (AP), OCT4 , SOX2 NANOG stably maintained over 33 passages, although plasmids remained detectable. vitro differentiation assessed by comparing this previously established (bFF-iPSCs mOSKM). Both lines exhibited downregulation upregulation early marker PAX3 . By day 30, formed elongated, multinucleated characteristic myotubes displayed corresponding gene expression profile. Discussion These results provide new insights into bovine myogenesis its application cultured meat production. While promising, also highlights difficulty achieving complete differentiation, indicating need optimization protocols.

Language: Английский

Food Safety Considerations in the Advancement of Cultured Meat: Evaluating Novel Ingredients DOI Creative Commons

Sawali Suhas Navare,

Ahmed Y. El-Moghazy

Future Foods, Journal Year: 2025, Volume and Issue: unknown, P. 100639 - 100639

Published: April 1, 2025

Language: Английский

Citations

0
Genome-scale metabolic models in cultivated meat: advances, challenges, and future directions DOI Creative Commons
Sandra Gomez Romero,

Isabella Spielmann,

Nanette R. Boyle

et al.

Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 94, P. 103313 - 103313

Published: May 15, 2025

Cultivated meat is an emerging field that integrates stem cell biology, tissue engineering, and bioprocessing to create a sustainable alternative conventional meat. However, its widespread adoption faces key metabolic challenges, including the need for serum-free media, optimization of line-specific growth conditions, enhanced cellular engineering strategies. GEnome-scale Metabolic models (GEMs) have been instrumental in optimizing conditions Chinese hamster ovaries, powerhouse species biomanufacturing, they can be adapted used cultivated same way. These facilitate transition cost-effective, media improve biomass production through flux analysis. Future advancements will require expanding publicly available data sets, refining models, integrating transcriptomic proteomic enhance predictive accuracy. By leveraging GEMs, overcome current limitations, paving way scalable, sustainable, economically viable traditional

Language: Английский

Citations

0
Generation of bovine iPSCs from fetal fibroblasts for in vitro myogenesis and cultured meat DOI Creative Commons
Kaiana Recchia, Methi Wathikthinnakon, Fabiana Fernandes Bressan

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: May 16, 2025

Introduction Emerging biotechnologies are increasingly being explored for food production, including the development of cell-cultivated meat. Conventional approaches typically rely on satellite cell (SC) biopsies, which present challenges in scalability. Bovine induced pluripotent stem cells (biPSCs) represent a promising alternative due to their capacity self-renewal and developmental plasticity. Methods This study utilized both lentiviral (integrating) episomal (non-integrating) reprogramming strategies generate biPSCs suitable myogenic differentiation. fetal fibroblasts (bFFs) were reprogrammed using vectors pMaster K pCXB-EBNA1, leading emergence putative iPSC colonies 13 days post-nucleofection. A clonal line, bFF-iPSCs pMK, was selected further analysis. Results The pMK line expressed key pluripotency markers alkaline phosphatase (AP), OCT4 , SOX2 NANOG stably maintained over 33 passages, although plasmids remained detectable. vitro differentiation assessed by comparing this previously established (bFF-iPSCs mOSKM). Both lines exhibited downregulation upregulation early marker PAX3 . By day 30, formed elongated, multinucleated characteristic myotubes displayed corresponding gene expression profile. Discussion These results provide new insights into bovine myogenesis its application cultured meat production. While promising, also highlights difficulty achieving complete differentiation, indicating need optimization protocols.

Language: Английский

Citations

0