Comparative analysis of five mitochondrial genomes of the subfamily Galerucinae (Coleoptera: Chrysomelidae) and evolution of control regions inferred from phylogeny DOI Creative Commons
Shiwen Xu, Pu Miao, Qiaoqiao Liu

et al.

Frontiers in Ecology and Evolution, Journal Year: 2025, Volume and Issue: 13

Published: April 28, 2025

Introduction Mitochondrial genome (mitogenome) is a widely utilized source of genetic data for animal species identification, molecular phylogeny, phylogeography, and evolutionary research at various taxonomic scales. With 14,500 recognized placed in over 1,100 genera, Galerucinae (Coleoptera: Chrysomelidae), also known as leaf beetles, the largest subfamily within Chrysomelidae. Methods We newly sequenced annotated five mitogenomes from Galerucinae. Additionally, we investigated their nucleotide composition, codon usage, relative synonymous usage rate protein-coding genes (PCGs). The phylogenetic relationship been inferred by gathering 97 GenBank our mitogenomes. divergence times were estimated using three calibration nodes MCMCTree. Furthermore, structural features control regions (CRs) framework Results discussion length ranged 15,737 to 16,484 bp. All these exhibited high AT content, well positive skew negative GC skew. Most PCGs have ATN start codons, TAA TAG most assigned stop codons. substitution analysis suggested that ATP8 experienced selection, while COX1 underwent purifying selection. Phylogenetic analyses showed both tribes Alticini Galerucini monophyletic sister groups. Within two tribes, groups subtribes monophyletic. Our time inference, dating origin Middle Cretaceous period (102.69 Ma), indicated main diverged occurred during Paleogene. Finally, revealed variations organization CRs even among closely related groups, which can be attributed specific elements.

Language: Английский

Evolutionary history and divergence times of Tettigoniidae (Orthoptera) inferred from mitochondrial phylogenomics DOI Creative Commons
Tian-You Zhao, Ziqi Lin,

Hailin Yang

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 13, 2025

Background Advances in high-throughput sequencing technology have led to a rapid increase the number of sequenced mitochondrial genomes (mitogenomes), ensuring emergence phylogenomics, as powerful tool for understanding evolutionary history various animal groups. Methods In this study, we utilized assemble and annotate mitogenomes Letana rubescens (Stål) Isopsera denticulata Ebner. We described characteristics genes these two species. Utilizing 13 PCGs 2 rRNA genes, reconstructed phylogenetic relationships Tettigoniidae by combining published data with our newly generated data. used likelihood mapping, signal-to-noise ratio (SNR), saturation analysis across different datasets ensure robustness inferred topologies. Results conclusion Selective pressure on protein-coding (PCGs) ribosomal RNA (rRNA) revealed that only ND1 COX1 contained positively selected sites, while negative selection dominated all indicating primarily function maintain genetic integrity. Additionally, assessed rates five major subfamilies using mean pairwise identity analysis. Phylogenetic results study provide more precise insights into within Tettigoniidae, spanning subfamilies, tribes, genera, further estimated divergence times four fossil calibration nodes MCMCTree, dating origin katydids early Paleogene period (approximately 60.86 Mya), identifying subfamilies.

Language: Английский

Citations

1
Comparative analysis of five mitochondrial genomes of the subfamily Galerucinae (Coleoptera: Chrysomelidae) and evolution of control regions inferred from phylogeny DOI Creative Commons
Shiwen Xu, Pu Miao, Qiaoqiao Liu

et al.

Frontiers in Ecology and Evolution, Journal Year: 2025, Volume and Issue: 13

Published: April 28, 2025

Introduction Mitochondrial genome (mitogenome) is a widely utilized source of genetic data for animal species identification, molecular phylogeny, phylogeography, and evolutionary research at various taxonomic scales. With 14,500 recognized placed in over 1,100 genera, Galerucinae (Coleoptera: Chrysomelidae), also known as leaf beetles, the largest subfamily within Chrysomelidae. Methods We newly sequenced annotated five mitogenomes from Galerucinae. Additionally, we investigated their nucleotide composition, codon usage, relative synonymous usage rate protein-coding genes (PCGs). The phylogenetic relationship been inferred by gathering 97 GenBank our mitogenomes. divergence times were estimated using three calibration nodes MCMCTree. Furthermore, structural features control regions (CRs) framework Results discussion length ranged 15,737 to 16,484 bp. All these exhibited high AT content, well positive skew negative GC skew. Most PCGs have ATN start codons, TAA TAG most assigned stop codons. substitution analysis suggested that ATP8 experienced selection, while COX1 underwent purifying selection. Phylogenetic analyses showed both tribes Alticini Galerucini monophyletic sister groups. Within two tribes, groups subtribes monophyletic. Our time inference, dating origin Middle Cretaceous period (102.69 Ma), indicated main diverged occurred during Paleogene. Finally, revealed variations organization CRs even among closely related groups, which can be attributed specific elements.

Language: Английский

Citations

0