P2X7 receptor antagonists modulate experimental autoimmune neuritis via regulation of NLRP3 inflammasome activation and Th17 and Th1 cell differentiation

Journal of Neuroinflammation, Journal Year: 2024, Volume and Issue: 21(1)

Published: March 25, 2024

Abstract Background Guillain–Barré syndrome (GBS), a post-infectious, immune-mediated, acute demyelinating disease of the peripheral nerves and nerve roots, represents most prevalent severe paralyzing neuropathy. Purinergic P2X7 receptors (P2X7R) play crucial role in central nervous system inflammation. However, little is known about their immune-inflammatory response within system. Methods Initially, we assessed expression purinergic P2X7R blood patients with GBS using flow cytometry qRT-PCR. Next, explored P2 X7R CD4 + T cells, CD8 macrophages sciatic spleens rats immunofluorescence labeling cytometry. The antagonist brilliant blue G (BBG) was employed to examine its therapeutic impact on experimental autoimmune neuritis (EAN) induced by immunization P0 180 − 199 peptide. We analyzed cell differentiation splenic mononuclear cells cytometry, Th17 through staining, examined pro-inflammatory cytokine mRNA RT-PCR. Additionally, performed protein blotting assess NLRP3-related inflammatory proteins nerve. Lastly, utilized NLRP3 EAN. Results elevated not only but also In EAN, inhibiting BBG alleviated neurological symptoms, reduced demyelination, decreased infiltration nerves, improved conduction. limited production molecules, down-regulated NLRP3, suppressed Th1 thus protecting against These effects collectively contribute modifying environment enhancing outcomes EAN rats. Conclusions Suppression relieved manifestation regulating inflammasome activation. This finding underscores potential significance as target for anti-inflammatory treatments, advancing research management GBS.

Language: Английский

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Guillain-Barré syndrome after surgery: a literature review

Frontiers in Neurology, Journal Year: 2024, Volume and Issue: 15

Published: April 4, 2024

Guillain-Barré syndrome (GBS) is a rare postoperative complication that sometimes characterized by serious motor weakness and prolonged weaning from mechanical ventilation. Although the exact nature of relationship between GBS surgical procedure still unclear, there clear increased incidence in post-surgical patients compared to non-surgical patients. after surgery unique several ways. The course unfolds more rapidly than other situations where develops, condition often severe, respiratory muscles are commonly involved. Prompt diagnosis appropriate treatment essential, can worsen if treated inappropriately. Postoperative sedation, intubation, restraint use make difficult, as onset symptoms or numbness those contexts not obvious. misdiagnosed, being attributed complications, subsequently mishandled. lack relevant information further obscures clinical picture. We sought better understand performing an analysis literature, focusing on clearly documenting characteristics, diagnosis, management emerges following surgery. underscore importance physicians aware possibility major variety laboratory investigations early suspected cases.

Language: Английский

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Sex differences in Guillain Barré syndrome, chronic inflammatory demyelinating polyradiculoneuropathy and experimental autoimmune neuritis

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 9, 2022

Guillain Barré syndrome (GBS) and its variants, chronic inflammatory demyelinating polyradiculoneuropathy (CIDP are regarded as immune mediated neuropathies. Unlike in many autoimmune disorders, GBS CIDP more common males than females. Sex is not a clear predictor of outcome. Experimental neuritis (EAN) an animal model these diseases, but there no studies the effects sex EAN. The pathogenesis involves response to non-protein antigens, antigen presentation through non-conventional T cells and, with nodopathy, IgG4 antibody responses antigens. There some reported differences elements system we speculate that could contribute male predominance suggest peripheral nerves topic worthy further study.

Language: Английский

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Infection, vaccination and narcolepsy type 1: Evidence and potential molecular mechanisms

Journal of Neuroimmunology, Journal Year: 2024, Volume and Issue: 393, P. 578383 - 578383

Published: June 1, 2024

Language: Английский

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Guillain-Barré syndrome

Evolution Medicine and Public Health, Journal Year: 2024, Volume and Issue: 12(1), P. 169 - 171

Published: Jan. 1, 2024

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