Biomedicines,
Journal Year:
2024,
Volume and Issue:
13(1), P. 10 - 10
Published: Dec. 25, 2024
Background/Objectives:
Peroxisome
proliferator-activated
receptor
gamma
(PPARγ)
is
a
fatty
acid-binding
transcription
activator
of
the
adipokine
chemerin.
The
key
role
PPARγ
in
adipogenesis
was
established
by
reports
on
adipose
tissue-resident
macrophages
that
express
PPARγ.
present
study
examined
PPARγ+
human
skeletal
muscle
tissues,
their
response
to
acid
(FA)
species,
and
correlations
with
age,
obesity,
expression,
an
abundance
other
macrophage
phenotypes.
Methods:
An
ex
vivo
model
surgical
specimens
were
maintained
without
or
FAs
for
up
11
days
utilized.
Immunofluorescence
analysis
used
detect
phenotypes
mitochondrial
activity.
Preconfigured
arrays
expression
34
different
adipokines
chemokines.
Results:
Data
from
14
adults
revealed
exclusively
reside
intermuscular
tissue
(IMAT),
correlates
metabolic
status
surrounding
adipocytes
during
maintenance
vitro
9-11
days.
Elevated
levels
lead
significant
increases
populations,
which
are
correlated
donor's
body
mass
index
(BMI).
Conclusions:
represent
distinctly
specialized
population
regulatory
cells
within
IMATs
accordance
status.
Thus,
future
in-depth
studies
IMAT-resident
action
mechanisms
will
elucidate
pathogenesis
dysfunction.
Journal of Hematology & Oncology,
Journal Year:
2024,
Volume and Issue:
17(1)
Published: Aug. 24, 2024
The
emergence
of
spatial
multi-omics
has
helped
address
the
limitations
single-cell
sequencing,
which
often
leads
to
loss
context
among
cell
populations.
Integrated
analysis
genome,
transcriptome,
proteome,
metabolome,
and
epigenome
enhanced
our
understanding
biology
molecular
basis
human
diseases.
Moreover,
this
approach
offers
profound
insights
into
interactions
between
intracellular
intercellular
mechanisms
involved
in
development,
physiology,
pathogenesis
In
comprehensive
review,
we
examine
current
advancements
technologies,
focusing
on
their
evolution
refinement
over
past
decade,
including
improvements
throughput
resolution,
modality
integration,
accuracy.
We
also
discuss
pivotal
contributions
revealing
heterogeneity,
constructing
detailed
atlases,
deciphering
crosstalk
tumor
immunology,
advancing
translational
research
cancer
therapy
through
precise
mapping.
Journal for ImmunoTherapy of Cancer,
Journal Year:
2024,
Volume and Issue:
12(11), P. e009494 - e009494
Published: Nov. 1, 2024
Background
Patient-derived
colorectal
cancer
(CRC)
organoids
(PDOs)
solely
consisting
of
malignant
cells
led
to
major
advances
in
the
understanding
treatments.
Yet,
a
limitation
is
absence
from
tumor
microenvironment,
thereby
prohibiting
potential
investigation
treatment
responses
on
immune
and
structural
cells.
Currently
there
are
sparse
reports
describing
interaction
PDOs,
cancer-associated
fibroblasts
(CAFs)
tumor-associated
macrophages
(TAMs)
complex
primary
co-culture
assay
systems.
Methods
Primary
PDOs
patient
matched
CAF
cultures
were
generated
surgical
resections.
Co-culture
systems
CAFs
monocytic
myeloid
set
up
recapitulate
features
seen
tumors.
Single-cell
transcriptomics
flow
cytometry
was
used
show
effects
culture
TAM
populations
assays
under
chemotherapeutic
oncolytic
viral
treatment.
Results
In
contrast
co-cultures
monocytes,
CAF/monocyte
CAF/monocyte/tumor
cell
triple
resulted
partial
differentiation
into
phenotypic
switch,
characterized
by
expression
immunosuppressive
markers
comparable
TAMs
CRC.
Oxaliplatin
5-fluorouracil,
standard-of-care
chemotherapy
for
CRC,
induced
polarization
pro-inflammatory
phenotype
immunogenic
with
an
virus.
Monitoring
phagocytosis
as
functional
proxy
macrophage
activation
subsequent
onset
response,
revealed
that
chemotherapy-induced
death,
but
not
virus-mediated
necessary
induce
CRC
Moreover,
enhanced
phagocytic
activity
treated
cultures.
Conclusions
CAF-containing
successfully
model
TAM-like
phenotypes
ex
vivo
allow
assessment
their
changes
response
treatments
following
precision
medicine
approach.
Virulence,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Nov. 13, 2024
Periodontitis
is
one
of
the
chronic
diseases
that
have
greatest
impact
on
human
health,
and
it
associated
with
several
other
diseases.
Tissue
damage
periodontitis
often
connected
immune
response.
Immune
cells
are
a
crucial
component
system
directly
involved
in
during
inflammatory
phase
disease.
Macrophages,
as
key
system,
responsible
for
defence,
antigen
presentation
phagocytosis
healthy
tissue.
They
also
closely
linked
to
development
resolution
periodontitis,
through
mechanisms
such
macrophage
polarization,
pattern
recognition
receptors
recognition,
efferocytosis,
Specialized
Pro-resolving
Mediators
(SPMs)
production.
Additionally,
apoptosis
autophagy
known
play
role
recovery
periodontitis.
This
review
aims
investigate
aforementioned
more
detail
identify
novel
therapeutic
approaches
Journal of Tissue Engineering,
Journal Year:
2025,
Volume and Issue:
16
Published: March 1, 2025
By
integrating
3D-inkjet
bioprinting
technology,
differentiated
human
cells
can
be
assembled
into
artificial
lung
tissue
structure
to
achieve
a
rapid,
efficient,
and
reproducible
disease
model
construction
process.
Here,
we
developed
novel
bioprinting-based
method
construct
(ALTs)
for
acute
injury
(ALI)
modeling,
research
application.
It
also
used
study
the
role
of
relevant
in
by
adjusting
cell
type
adapted
bio-functions
immune
during
cell-cell
interactions.
Firstly,
series
process
optimizations
were
done
mass-produce
alginate
hydrogel
microspheres
(Alg)
with
particle
size
262.63
±
5
μm
using
3D
bioprinter,
then
I
collagen
polydopamine
deposited
turns
adhesion
layer
on
surfaces
Alg
(P-Alg)
was
increased
328.41
3.81
μm.
This
platform
exhibites
good
stability,
timescale-dependent
behavior,
long-term
adhesion.
Subsequently,
several
including
endothelial,
epithelial,
fibroblast,
even
such
as
macrophages
adhered
P-Alg
through
rotational
culture,
leading
contractions
aggregation,
subsequently
formed
ALTs
or
(ALTs@M)
alveolar-like
structure.
Finally,
successfully
constructed
an
ALI
barrier
damage
lipopolysaccharide
stimulation
vitro,
comparison
secreted
inflammatory
factors
between
ALTs@M.
Results
demonstrated
that
ALTs@M
more
effective
than
stimulating
microenvironment
lungs,
providing
vitro
cellular
interactions
macrophage
research.
Altogether,
this
strategy
technology
allowed
flexible
development
structures
potential
models
preclinical
studies.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(9), P. 4287 - 4287
Published: May 1, 2025
Psoriasis
and
atopic
dermatitis
(AD)
are
both
chronic
inflammatory
skin
diseases.
Their
pathogenesis
remains
incompletely
understood.
The
polarization
states
of
macrophages,
as
a
crucial
part
the
innate
immune
system,
influenced
by
various
factors
such
cytokines,
mediators,
epigenetics.
Research
has
demonstrated
that
macrophages
play
“double-edged
sword”
role
in
pathological
process
diseases:
they
drive
inflammation
progression
participate
tissue
repair.
This
article
summarizes
roles
development
homeostasis
psoriasis
dermatitis.
It
explores
impact
different
on
In
conclusion,
understanding
classification
plasticity
is
for
deeper
AD
personalized
treatments.
Frontiers in Immunology,
Journal Year:
2025,
Volume and Issue:
16
Published: April 2, 2025
Pregnancy
involves
complex
physiological
adaptations
across
maternal
organs
and
the
immune
system
to
support
fetal
development.
Macrophages
play
a
dual
role
during
pregnancy:
defending
against
pathogens
supporting
tissue
adaptation.
However,
comprehensive
in-depth
studies
of
cross-tissue
transcriptional
heterogeneity
macrophages
healthy
pregnancy
at
single-cell
level
remain
elusive.
We
performed
RNA
sequencing
(scRNA-seq)
profile
from
pregnant
pig
49
tissues.
Immunofluorescence
was
verify
specific
expression
transcription
factors.
In
this
study,
we
generated
macrophage
atlas
containing
114,881
tissues/organs
within
one
single
pig,
identified
33
subtypes,
revealed
extensive
tissue-specific
diversity.
observed
significant
subtypes
five
different
anatomical
sites
adipose
tissue.
Notably,
Mφ
MARCO+
subtype,
primarily
derived
mesenteric
tissue,
showed
higher
activity
in
pattern
recognition
receptor
signaling
pathways
compared
other
tissues,
including
fat
depots.
Cross-tissue
analysis
distinct
patterns
factors,
cytokines,
cell
surface
receptors,
factor
PLSCR1,
specifically
expressed
lung
verified
by
immunofluorescence.
Cross-species
unveiled
conservation
among
pigs,
humans,
mice.
constructed
multiple-tissue
transcriptome
revealing
their
molecular
differences
commonalities
tissues
species.
Our
study
provides
valuable
resource
for
understanding
diversity
pigs.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(19), P. 10722 - 10722
Published: Oct. 5, 2024
Human
skeletal
muscle
contains
different
types
of
tissues
with
fibers
(SMFs)
and
intermuscular
adipose
(IMATs)
as
the
main
components.
We
maintained
human
from
12
study
participants
under
native
conditions
in
vitro
for
11
days
to
investigate
dynamics
macrophages
that
reside
adjacent
IMATs
SMFs
simultaneously.
The
samples
were
subjected
immunohistochemical
analysis
macrophage
phenotyping
mitochondrial
mass
assessment
before
after
maintenance
vitro.
Multiplex
protein
was
used
determine
cytokine/chemokine
expression
tissue
extracts.
results
revealed
significant
correlations
between
donor
age
or
body
index
(BMI)
distinct
phenotypes
resident
IMATs.
SMF-
IMAT-resident
differed
significantly
exhibited
inverse
chemokine/cytokine
levels
activity.
Moreover,
responses
saturated
unsaturated
fatty
acids
(FAs)
substantially
These
findings
showed
functional
diversity
phenotypically
identical
niches.
Thus,
currently
available
markers
cannot
capture
tissue-resident
macrophages.
model
present
may
help
elucidate
how
affect
homeostasis
disease
humans.
Cancer Medicine,
Journal Year:
2024,
Volume and Issue:
13(21)
Published: Nov. 1, 2024
ABSTRACT
Introduction
Macrophages
are
essential
in
maintaining
homeostasis,
combating
infections,
and
influencing
the
process
of
various
diseases,
including
cancer.
originate
from
diverse
lineages:
Notably,
tissue‐resident
macrophages
(TRMs)
differ
hematopoietic
stem
cells
circulating
monocyte‐derived
based
on
genetics,
development,
function.
Therefore,
understanding
recruited
TRM
populations
is
crucial
for
investigating
disease
processes.
Methods
By
searching
literature
databses,
we
summarized
recent
relevant
studies.
Research
has
shown
that
tumor‐associated
(TAMs)
distinct
origins
accumulate
tumor
microenvironment
(TME),
with
TRM‐derived
TAMs
closely
resembling
gene
signatures
normal
TRMs.
Results
Recent
studies
have
revealed
TRMs
play
a
role
cancer
progression.
However,
organ‐specific
effects
complicate
investigations.
Nonetheless,
precise
involvement
tumors
unclear.
This
review
explores
multifaceted
roles
cancer,
presenting
insights
into
their
origins,
proliferation,
latest
research
methodologies,
impact
across
sites,
potential
strategies
as
therapeutic
targets,
interactions
other
within
TME,
internal
heterogeneity
Conclusions
We
believe
comprehensive
will
pave
way
targeted
therapies
treatment
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(22), P. 12330 - 12330
Published: Nov. 17, 2024
Macrophage
polarization
critically
contributes
to
a
multitude
of
human
pathologies.
Hence,
modulating
macrophage
is
promising
approach
with
enormous
therapeutic
potential.
Macrophages
are
characterized
by
remarkable
functional
and
phenotypic
plasticity,
pro-inflammatory
(M1)
anti-inflammatory
(M2)
states
at
the
extremes
multidimensional
spectrum.
Cell
morphology
major
indicator
for
activation,
describing
M1(-like)
(rounded)
M2(-like)
(elongated)
different
cell
shapes.
Here,
we
introduced
painting
macrophages
better
reflect
their
multifaceted
plasticity
associated
phenotypes
beyond
rigid
dichotomous
M1/M2
classification.
Using
high-content
imaging,
established
deep
learning-
feature-based
image
analysis
tools
elucidate
cellular
fingerprints
that
inform
about
subtle
blood
monocyte-derived
iPSC-derived
as
screening
surrogate.
Moreover,
show
feature
profiling
suitable
identifying
inter-donor
variance
describe
relevance
'cell
roundness'
dissect
distinct
signatures
after
stimulation
known
biological
or
small-molecule
modulators
(re-)polarization.
Our
novel
AI-fueled
provide
resource
high-content-based
drug
candidate
profiling,
which
set
stage
(re-)polarization
in
health
disease.
