Deep immune profiling of chronic rhinosinusitis in allergic and non-allergic cohorts using mass cytometry DOI Open Access

Fana Alem Kidane,

Lena Müller, Marianne Rocha-Hasler

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 22, 2023

ABSTRACT Background Chronic rhinosinusitis (CRS) is characterized by persistent nasal and paranasal sinus mucosa inflammation. It comprises two phenotypes, namely CRS with polyps (CRSwNP) without (CRSsNP). CRSwNP can be associated asthma hypersensitivity to non-steroidal anti-inflammatory drugs (NSAIDs) in a syndrome known as NSAID-exacerbated respiratory disease (N-ERD). Furthermore, frequently intertwines allergies. Objective This study investigated the phenotypic characteristics of peripheral blood mononuclear cells (PBMCs) within cohorts patients, additionally examining influence comorbid allergies on these parameters. Methods 24 participants were grouped into controls, CRSsNP, CRSwNP, N-ERD ( n=6 /group), half patients each group having Levels cytokines quantified secretions sera. The abundance features immune PBMCs evaluated through mass cytometry clustering methods. Results showed heightened type 2 cytokine levels. Mass analysis revealed increased activated naive B cell levels N-ERD, while resting higher CRSsNP. Th2a did not differ between subtypes but significantly elevated allergic subjects. In had lower CXCR5 CD45RA expression, NK displayed reduced CD56 Conclusions There are distinct immunological phenotypes allergy, Capsule summary examines profiles different allergy highlighting intricacies differences allergy.

Language: Английский

Immune signatures predict response to house dust mite subcutaneous immunotherapy in patients with allergic rhinitis DOI
Nan Wang, Jia Song,

Shi‐Ran Sun

et al.

Allergy, Journal Year: 2024, Volume and Issue: 79(5), P. 1230 - 1241

Published: Feb. 25, 2024

Identifying predictive biomarkers for allergen immunotherapy response is crucial enhancing clinical efficacy. This study aims to identify such in patients with allergic rhinitis (AR) undergoing subcutaneous (SCIT) house dust mite allergy.

Language: Английский

Citations

9
Dupilumab treatment decreases MBC2s, correlating with reduced IgE levels in pediatric atopic dermatitis DOI Creative Commons
Margot E Starrenburg, Manal Bel Imam, Juan Felipe Valdés‐López

et al.

Journal of Allergy and Clinical Immunology, Journal Year: 2024, Volume and Issue: 154(5), P. 1333 - 1338.e4

Published: July 20, 2024

BackgroundA preference for type 2 immunity plays a central role in the pathogenesis of atopic dermatitis (AD). Dupilumab, an mAb targeting IL-4 receptor α (IL-4Rα) subunit, inhibits and IL-13 signaling. These cytokines contribute significantly to IgE class switch recombination B cells, critical diseases. Recent studies indicate IgG+CD23hiIL-4Rα+ memory cells (MBC2s) as IgE-producing B-cell precursors, linked total serum levels patients. Total decreased during dupilumab treatment previous studies.ObjectiveWe sought assess effects comparison with alternative therapies on frequency MBC2s correlation pediatric patients AD.MethodsPediatric AD, participating ongoing trial, underwent randomization into 3 groups: (n = 12), cyclosporine topical 12). Plasma samples PBMCs were collected at baseline (T0) 6 months after starting therapy (T6). Flow cytometry was used PBMC phenotyping, ELISA plasma.ResultsOur findings revealed significant reduction MBC2 among treated dupilumab. In addition, observed between levels.ConclusionsSystemic blocking IL-4Rα subunit leads decrease circulating AD. Our unveiled novel mechanism through which exerts its influence signature.

Language: Английский

Citations

4
Comprehensive Single-Cell Profiling of T and B Cell Subsets in Mice Reveals Impacts on Memory Immune Responses in FMDV Infection DOI

Wei Zhao,

Jige Du,

Jia En Su

et al.

Virology, Journal Year: 2025, Volume and Issue: 606, P. 110496 - 110496

Published: March 12, 2025

Language: Английский

Citations

0
Revisiting Migraine Pathophysiology: from Neurons To Immune Cells Through Lens of Immune Regulatory Pathways DOI

Sugumar Subalakshmi,

Rapuru Rushendran, Chitra Vellapandian

et al.

Journal of Neuroimmune Pharmacology, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 2, 2025

Language: Английский

Citations

0
Recombinant Hypoallergenic Cat Allergy Vaccines DOI Creative Commons
Daria Trifonova, Mirela Curin, Margarete Focke‐Tejkl

et al.

Allergy, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Molecular forms of allergen-specific immunotherapy (AIT) for cat allergy are needed. Fel d 1, 4, and 7 the most important allergens. IgE epitopes 4 were mapped by blocking allergic patients' binding with allergen peptide-specific antisera. Five recombinant fusion proteins (PreS-Cat 1-PreS-Cat 5) each containing hepatitis B virus (HBV)-derived PreS as an immunological carrier non-allergenic peptides from sites expressed in Escherichia coli, purified, characterized mass spectrometry, circular dichroism (CD), size exclusion chromatography. ImmunoCAP basophil activation experiments demonstrated hypoallergenic activity PreS-Cat 1-5. The ability 1-5 to induce IgE-blocking antibodies rabbits was compared three licensed extract-based AIT vaccines. 1-5-specific IgG tested inhibition specific activation. T cell induction cytokine secretion allergens PBMC cultures. Recombinant hypoallergenic, biochemically structurally defined obtained. Two subcutaneous immunizations induced equal (Fel 1) or better 7) 5 > 1 3) than six fifteen PreS-Cat-specific strongly inhibited significantly more IL-10 cultured PBMCs patients highly promising molecular vaccine candidates allergy, combining 1-, 4-, 7-peptides single proteins.

Language: Английский

Citations

0
Extracellular Vesicles in Asthma: Intercellular Cross-Talk in TH2 Inflammation DOI Creative Commons
Naila Arif Cheema, Annalisa Castagna,

Francesca Ambrosani

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 542 - 542

Published: April 3, 2025

Asthma is a complex, multifactorial inflammatory disorder of the airways, characterized by recurrent symptoms and variable airflow obstruction. So far, two main asthma endotypes have been identified, type 2 (T2)-high or T2-low, based on underlying immunological mechanisms. Recently, extracellular vesicles (EVs), particularly exosomes, gained increasing attention due to their pivotal role in intercellular communication distal signaling modulation. In context pathobiology, an amount experimental evidence suggests that EVs secreted eosinophils, mast cells, dendritic T neutrophils, macrophages, epithelial cells contribute disease This review explores profiling molecular signatures T2-high T2-low asthma, offering novel perspectives mechanisms potential therapeutic targets.

Language: Английский

Citations

0
Recent advances in regulatory immune cells: exploring the world beyond Tregs DOI Creative Commons
Peng Shi, Yi Yu,

Hongwei Xie

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 16, 2025

Regulatory immune cells are pivotal in maintaining homeostasis and modulating responses to prevent pathologies. While T regulatory (Tregs) extensively recognized for their immunosuppressive roles, emerging subsets of cells, including CD8+ (CD8+Tregs) B (Bregs), myeloid-derived suppressor (MDSCs), dendritic (DCregs), innate lymphoid (ILCregs), natural killer (NKregs), garnering increased attention. This review delves into the phenotypic characteristics, mechanisms action, immune-regulatory functions these lesser-known but crucial cell subsets. The provides a comprehensive examination each type, detailing origins, unique functionalities, contributions homeostasis. It emphasizes complex interplay among how coordinated activities influence diverse pathological therapeutic contexts, autoimmunity, cancer immunotherapy, chronic inflammation, transplant tolerance. By unraveling mechanisms, outlines novel avenues, such as targeting modulate activity enhance precision medicine approaches. future immunotherapy modulation lies leveraging expanded knowledge presenting challenges opportunities clinical applications.

Language: Английский

Citations

0
Deep immune profiling of chronic rhinosinusitis in allergic and non-allergic cohorts using mass cytometry DOI Creative Commons

Fana Alem Kidane,

Lena Müller, Marianne Rocha-Hasler

et al.

Clinical Immunology, Journal Year: 2024, Volume and Issue: 262, P. 110174 - 110174

Published: March 8, 2024

Chronic rhinosinusitis (CRS) is a persistent nasal and paranasal sinus mucosa inflammation comprising two phenotypes, namely CRS with polyps (CRSwNP) without (CRSsNP). CRSwNP can be associated asthma hypersensitivity to non-steroidal anti-inflammatory drug (NSAID) in syndrome known as NSAID-exacerbated respiratory disease (N-ERD). Furthermore, frequently intertwines allergies. This study investigated levels of 33 different serum cytokines phenotypic characteristics peripheral blood mononuclear cells (PBMCs) within cohorts patients (n = 24), additionally examining the influence comorbid allergies by mass cytometry. N-ERD showed heightened type 2 cytokine levels. Mass cytometry revealed increased activated naive B cell N-ERD, while resting were higher CRSsNP. Th2a significantly elevated allergic subjects, but not groups. In conclusion, there are distinct immunological features PBMCs phenotypes allergy.

Language: Английский

Citations

3
Single‐cell RNA sequencing reveals the landscape of biomarker in allergic rhinitis patient undergoing intracervical lymphatic immunotherapy and related pan‐cancer analysis DOI
Li Yin, Hao Li, Weijun Huang

et al.

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 39(5), P. 2817 - 2829

Published: Jan. 30, 2024

Abstract Introduction Allergic rhinitis (AR) is one of the leading allergic diseases worldwide. Allergen immunotherapy (AIT) induces persistent specific allergen tolerance to achieve remission symptoms in AR patients. We creatively conducted intra‐cervical lymphatic (ICLIT) for However, underlying molecular mechanism immune cell response AIT remains elusive. Method To investigate transcriptome profile patients who underwent ICLIT, we comprehensively investigated transcriptional changes B cells from peripheral blood mononuclear patient by single‐cell RNA sequencing. Immunoglobulins and relative key gene, which influences differentiation, was demonstrated. The biomarkers' association with different types tumors investigated. Results Naive cells, germinal center activated memory constituted subsets. expression IGHE , IGHGs IGHA IGHD IGHM validated. Pseudotime analysis further indicated dynamic change immunoglobulins suggesting that ITGB1 may contribute differentiation procedure cells. cell–cell communication among these demonstrated significantly enhanced CD23, BTLA signaling after ICLIT patient. upregulated 13 downregulated six others. High linked poor prognosis eight tumors. showed correlations tumor mutation burden, tissue purity, microsatellite instability Discussion as a potential biomarker significant identifying infiltration predicting prognosis.

Language: Английский

Citations

2
IgG from Dermatophagoides pteronyssinus (Der p)-atopic individuals modulates non-atopic thymic B cell phenotype (alfa-4/beta-7) and cytokine production (IFN-γ, IL-9, and IL-10) with direct membrane interaction DOI Creative Commons

Daniela Terra de-Apoena Reche,

Nicolle Rakanidis Machado, Beatriz Oliveira Fagundes

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 27, 2024

Abstract Studies about thymic B cells are scarce in the literature, but it was suggested that they can exert modulatory and regulatory functions on immune system. Thymic play some role regulating most frequent allergic background worldwide, atopy induced by mite Dermatophagoides pteronyssinus (Der p). Here, we aimed to evaluate if polyclonal IgG repertoire produced Der p-atopic individuals influence homing cytokine profile of human derived from non-atopic children aged less than seven days. With this purpose, formulations cultivated thymocytes their presence. We also assessed subclasses direct interaction with cell membranes. Our results could demonstrate not reduce expression α4β7 molecule as observed response other frequency IFN-γ- IL-9-producing compared mock condition. induce IL-10-producing control conditions. The failed diminish population IL-13-producing cells, unlike reduction when All had similar levels directly interacted Finally, performed experiments using peripheral where effects were observed. In conclusion, our observation demonstrates modulate potentially generating prone allergy development, which seems occur mature cells.

Language: Английский

Citations

1