Spatial characterization of interface dermatitis in cutaneous lupus reveals novel chemokine ligand-receptor pairs that drive disease DOI Creative Commons
Saeed Shakiba, Nazgol‐Sadat Haddadi, Khashayar Afshari

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 6, 2024

Abstract Chemokines play critical roles in the recruitment and activation of immune cells both homeostatic pathologic conditions. Here, we examined chemokine ligand-receptor pairs to better understand immunopathogenesis cutaneous lupus erythematosus (CLE), a complex autoimmune connective tissue disorder. We used suction blister biopsies measure cellular infiltrates with spectral flow cytometry interface dermatitis reaction, as well 184 protein analytes interstitial skin fluid using Olink targeted proteomics. Flow data concordantly demonstrated significant increases T antigen presenting (APCs). also performed spatial transcriptomics proteomics punch digital profiling (DSP) technology on CLE healthy margin controls examine discreet locations within tissue. Spatial confirmed elevation interferon (IFN) IFN-inducible CXCR3 ligands. Comparing involved versus uninvolved keratinocytes samples revealed upregulation essential inflammatory response genes areas near dermatitis, including AIM2 . Our Caspase 8, IL-18 which is final product activation, induced chemokines CCL8 CXCL6 lesional samples. Chemotaxis assays PBMCs from donors that are equally poised respond ligands, whereas CD14+CD16+ APC populations more sensitive via CXCR1 CD14+ CCR2. Taken together, our map pathway keratinocyte injury lymphocyte AIM2-Casp8-IL-18-CXCL6/CXCR1 CCL8/CCR2, IFNG/IFNL1-CXCL9/CXCL11-CXCR3. One Sentence Summary mapped orchestrators approaches archival fresh tissues.

Language: Английский

Type 2 immunity in allergic diseases DOI Creative Commons
İsmail Öğülür, Yasutaka Mitamura,

Duygu Yazıcı

et al.

Cellular and Molecular Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Language: Английский

Citations

4
Atopic Dermatitis Itch: Scratching for an Explanation DOI
Georgia Biazus Soares, Takashi Hashimoto, Gil Yosipovitch

et al.

Journal of Investigative Dermatology, Journal Year: 2024, Volume and Issue: 144(5), P. 978 - 988

Published: Feb. 16, 2024

Language: Английский

Citations

10
Sequential immunotherapy: towards cures for autoimmunity DOI
Francisco Ramírez‐Valle, Joseph Maranville,

Sophie Roy

et al.

Nature Reviews Drug Discovery, Journal Year: 2024, Volume and Issue: 23(7), P. 501 - 524

Published: June 5, 2024

Language: Английский

Citations

10
Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway DOI Creative Commons
Ga Hee Jeong,

Ki-Chan Kim,

Ji Hyun Lee

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(2), P. 83 - 83

Published: Jan. 9, 2025

Cannabinoid compounds have potential as treatments for a variety of conditions, with cannabigerol (CBG) being known its anti-inflammatory properties. In this study, we investigated the effects CBG in cellular model 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD). model, confirmed cytotoxicity and downregulated expression inflammatory markers CCL26, IL1B, IL6, TNF (p < 0.001). mouse clinical, histological, immunological changes were analyzed. The results showed that improved severity score, epidermal thickness, mast cell count reduced cytokines (Tslp, Il1b, Il4, Il6, Il13, Il17, Il18, Il22, Il33) by qRT-PCR Western blot modulated JAK1, JAK2, TYK2, STAT1, STAT2, STAT3, p-STAT3, STAT6, p-STAT6 0.05). Subsequently, p-IκBα, NF-κB, p-NF-κB signaling factors also 0.05), corresponding skin barrier factors. study indicate effectively alleviates AD-like symptoms suggest therapeutic agent.

Language: Английский

Citations

1
Neuroimmune mechanisms of type 2 inflammation in the skin and lung DOI Creative Commons
Masato Tamari, Aaron Ver Heul

Allergology International, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Type 2 inflammation has a major role in barrier tissues such as the skin and airways underlies common conditions including atopic dermatitis (AD) asthma. Cytokines interleukin 4 (IL-4), IL-5, IL-13 are key immune signatures of type targets multiple specific therapeutics for allergic diseases. Despite shared core mechanisms, distinct structures functions lead to unique therapeutic responses. It is increasingly recognized that nervous system sensing directing inflammatory processes. Indeed, crosstalk between activation somatosensory mediates tissue-specific itching skin. However, neuroimmune interactions shaped by neuronal landscapes, differ airways. In skin, dorsal root ganglia-derived neurons mediate pruritus via cytokines neurogenic mast cell or basophil activation. Conversely, vagal regulate airway responses releasing neuropeptides/neurotransmitters calcitonin gene-related peptides, neuromedin U, acetylcholine, noradrenaline. Sensory neuron-derived vasoactive intestinal peptide forms feedback loop with amplifying eosinophilic airways, mechanism absent These differences influence efficacy cytokine-targeted therapies. For instance, IL-4/IL-13-targeted therapies like dupilumab demonstrate AD diseases, whereas IL-5-targeted effective asthma but not AD. Understanding these underscores need tailored approaches address diseases where involved.

Language: Английский

Citations

1
Molecular mechanisms of pruritus in prurigo nodularis DOI Creative Commons
Yixin Shao, Duoqin Wang,

Yiqi Zhu

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 23, 2023

Pruritus is the most common symptom of dermatological disorders, and prurigo nodularis (PN) notorious for intractable severe itching. Conventional treatments often yield disappointing outcomes, significantly affecting patients’ quality life psychological well-being. The pathogenesis PN associated with a self-sustained “itch-scratch” vicious cycle. Recent investigations PN-related itch have partially revealed intricate interactions within cutaneous neuroimmune network; however, underlying mechanism remains undetermined. Itch mediators play key role in pruritus amplification understanding their action will undoubtedly lead to development novel targeted antipruritic agents. In this review, we describe series pruritogens receptors involved mediating itching PN, including cytokines, neuropeptides, extracellular matrix proteins, vasculogenic substances, ion channels, intracellular signaling pathways. Moreover, provide prospective outlook on potential therapies based existing findings.

Language: Английский

Citations

17
Crosstalk between ROS-inflammatory gene expression axis in the progression of lung disorders DOI
Sumel Ashique, Neeraj Mishra, Shubhrajit Mantry

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Language: Английский

Citations

6
The last step to achieve barrier damage control DOI Creative Commons
Ilaria Baglivo,

Stefania Colantuono,

Arianna Lumaca

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 13, 2024

Heterogeneity characterises inflammatory diseases and different phenotypes endotypes have been identified. Both innate adaptive immunity contribute to the immunopathological mechanism of these barrier damage plays a prominent role triggering type 2 inflammation through alarmins system, such as anti-Thymic Stromal Lymphopoietin (TSLP). Treatment with anti-TSLP monoclonal antibodies showed efficacy in severe asthma clinical trials for other eosinophilic are ongoing. The aim this perspective review is analyse current advances future applications TSLP inhibition control damage.

Language: Английский

Citations

4
Diagnosis and management of food allergy‐induced constipation in young children—An EAACI position paper DOI
Rosan Meyer, Yvan Vandenplas, Adriana Chebar Lozinsky

et al.

Pediatric Allergy and Immunology, Journal Year: 2024, Volume and Issue: 35(6)

Published: June 1, 2024

Abstract The recognition of constipation as a possible non‐Immunoglobulin E (IgE)‐mediated allergic condition is challenging because functional (unrelated to food allergies) common health problem with reported worldwide prevalence rate up 32.2% in children. However, many studies children report challenge proven cow's milk allergy and primary symptom have found that between 28% 78% improve on elimination diet. Due the paucity data focus IgE‐mediated allergy, not all guidelines list allergy. Yet, it included available English language. Exploring Non‐IgE‐Mediated Allergy (ENIGMA) Task Force (TF) European Academy for Clinical Immunology (EAACI) considers this paper context failure standard treatment discuss role allergens culprit This position used Delphi approach reaching consensus both diagnosis management, currently published are insufficient support systematic review.

Language: Английский

Citations

4
Beyond classical immunity: Mast cells as signal converters between tissues and neurons DOI Creative Commons
Thomas Plum, Thorsten B. Feyerabend, Hans‐Reimer Rodewald

et al.

Immunity, Journal Year: 2024, Volume and Issue: 57(12), P. 2723 - 2736

Published: Dec. 1, 2024

Language: Английский

Citations

4