Mechano‐induced arachidonic acid metabolism promotes keratinocyte proliferation through cPLA2 activity regulation DOI
Shengzhou Shan, Rui Jin, X. Cheng

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(23)

Published: Dec. 5, 2024

Abstract Mechano‐induced keratinocyte hyperproliferation is reported to be associated with various skin diseases. Enhanced cell proliferation often requires the active metabolism of nutrients produce energy. However, how keratinocytes adapt their cellular homeostasis mechanical cues remains unclear. Here, we first found that stretched showed accumulation metabolic arachidonic acid by metabolomic analysis. Second, stretch promoted through activation cytosolic calcium‐dependent phospholipase A2 (cPLA2). Knockdown or inhibition cPLA2 could reduce release and inhibit in vitro vivo. Third, analyzing overlapping transcriptomes acid‐stimulated keratinocytes, identified upregulation hexokinase domain‐containing protein 1 (HKDC1) expression, a novel gene involved glucose metabolism, which was acid‐induced during stretching. Our data reveal regulation mechanism induces proliferation, thereby coupling mechanics microenvironment. Strategies change process may lead new way treat diseases are related biophysical forces.

Language: Английский

Topical Steroid Withdrawal Is a Targetable Excess of Mitochondrial NAD+ DOI
Nadia Shobnam, Grace Ratley,

Sarini Saksena

et al.

Journal of Investigative Dermatology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

2
An epidermal serine sensing system for skin healthcare DOI Creative Commons

Ying Yuan,

Bowen Zhong,

Xiaokun Qin

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 18, 2025

Portable biosensors mainly focus on detecting biomarkers in biofluids but neglect the abundant skin stratum corneum, which are associated with functionality and integrity of barrier. Here, we propose a sensing patch designed for direct sampling situ quantification epidermal serine, an important biomarker healthcare. The consists porous hydrogel serine diffusion ion conduction, molecular imprinted polymer-based electrochemical sensor. By integrating customized handheld tester, system enables measurement levels. We demonstrate application this assessing moisturizing effect skincare product tracking recovery progress barrier function patient atopic dermatitis. Our work opens up potential scenario portable personalized Serine is authors introduce capable

Language: Английский

Citations

0
Time-of-day dependent promotion of keratinocyte differentiation by Cinnamomum cassia bark extract through the p38 MAPK Pathway DOI Creative Commons
Miji Yeom,

Kyungeun Jeon,

Dehun Ryu

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0318360 - e0318360

Published: March 18, 2025

The skin serves as an essential barrier against pathogens and external insults, preventing moisture loss. Chronic conditions such atopic dermatitis stem from impairments in function. Circadian rhythms affect blood flow characteristics, which are significant for understanding dermatitis. Cinnamomum cassia bark, commonly known cinnamon, is extensively utilized both modern Traditional Chinese Medicine its therapeutic properties managing chronic diseases. This study aimed to investigate the potential use of bark enhancing We examined impact extract (CCBE) on circadian clock-mediated enhancement barrier. CCBE enhanced expression keratinocyte differentiation markers, including keratin 10, filaggrin, caspase 14, calpain-1. also increased production hyaluronic acid protein. Additionally, improved rhythm period regulator 2 ( PER2 ). Notably, upregulated markers specifically during morning hours. Furthermore, we discovered that siRNA-mediated knockdown diminished increase induced by CCBE. These findings demonstrate can regulate keratinocytes a time-of-day-dependent manner via clock. augmented phosphorylation p38 JNK, while CCBE-induced FLG was reduced MAPK inhibitors. results suggest strengthen diurnally pathway, representing novel more effective method function accommodates daily variations properties.

Language: Английский

Citations

0
The humanistic burden of atopic dermatitis in Greece: a cross-sectional study DOI Creative Commons
Georgia Kourlaba,

Katerina Lioliou,

Garyfallia Stefanou

et al.

Dermatology Reports, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

The purpose of this study was to evaluate the quality life (QoL) and psychosocial burden in adult Greek patients with atopic dermatitis (AD) using validated tools investigate factors that influence AD’s humanistic burden. This observational, cross-sectional conducted Greece (January-September 2023) 150 members patient association “EPIDERMIA”, all diagnosed AD. Data collected via a structured questionnaire, including socio-demographic details, clinical history, AD severity (using Patient-Oriented Eczema Measure [POEM]), QoL, sleep disorders, psychological health. Based on POEM scores, 11% participants had clear/almost clear skin, 27% mild eczema, 51% moderate 12% severe/very severe eczema. or very/extremely large impact QoL for 29% 42% participants, respectively. Insomnia affected 55%, while 31% anxiety, 23% moderate/severe 10% depression, 8% moderately severe/severe depression. associated reduced higher rates insomnia, Our highlights significant associations between severity, factors, emphasizing need comprehensive management strategies.

Language: Английский

Citations

0
Atopic dermatitis and food allergy: More than sensitization DOI Creative Commons

K. Davis,

Estefania Claudio-Etienne,

Pamela A Frischmeyer-Guerrerio

et al.

Mucosal Immunology, Journal Year: 2024, Volume and Issue: 17(5), P. 1128 - 1140

Published: June 19, 2024

Language: Английский

Citations

3
Mechano‐induced arachidonic acid metabolism promotes keratinocyte proliferation through cPLA2 activity regulation DOI
Shengzhou Shan, Rui Jin, X. Cheng

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(23)

Published: Dec. 5, 2024

Abstract Mechano‐induced keratinocyte hyperproliferation is reported to be associated with various skin diseases. Enhanced cell proliferation often requires the active metabolism of nutrients produce energy. However, how keratinocytes adapt their cellular homeostasis mechanical cues remains unclear. Here, we first found that stretched showed accumulation metabolic arachidonic acid by metabolomic analysis. Second, stretch promoted through activation cytosolic calcium‐dependent phospholipase A2 (cPLA2). Knockdown or inhibition cPLA2 could reduce release and inhibit in vitro vivo. Third, analyzing overlapping transcriptomes acid‐stimulated keratinocytes, identified upregulation hexokinase domain‐containing protein 1 (HKDC1) expression, a novel gene involved glucose metabolism, which was acid‐induced during stretching. Our data reveal regulation mechanism induces proliferation, thereby coupling mechanics microenvironment. Strategies change process may lead new way treat diseases are related biophysical forces.

Language: Английский

Citations

1