Use of Antibody Structural Information in Disease Prediction Models Reveals Antigen Specific B Cell Receptor Sequences in Bulk Repertoire Data DOI Creative Commons
Onyekachi Nwogu, Kirandeep K. Gill,

Carolina Moore

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Convergent antibodies are highly similar elicited in multiple individuals response to the same antigen. provide insight into shared immunological responses and show great promise as diagnostic biomarkers. They have typically been identified using methods that consider amino acid sequence of third complementarity-determining region (CDR3) immunoglobulin heavy chain (IgH). In this study, we extend definition convergent use structural information about three IgH CDR regions (CDR1-3). We benchmark performance both definitions convergence by their ability predict disease status from bulk sequencing data for two different diseases (HIV infection food sensitization). predicted outperforms prior approaches prediction sensitization performs on par HIV status. Additionally, structurally antibody groups driving known binders. Thus, allows identification antigen specific data.

Language: Английский

Screening and Interaction Analysis of Shark-Derived Nanobodies against Crayfish Major Allergen Pro c 2 DOI
Yang Yang,

Xinrong He,

Si‐Yang He

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Pro c 2 (arginine kinase) is a major allergen in crayfish (Procambarus clarkii). Shark-derived variable domains of new antigen receptors (VNARs) have advantages developing detection and immunotherapy. This study constructed VNAR domain library from Chiloscyllium plagiosum immunized with 2. Three VNARs (VNAR-11, VNAR-20, VNAR-29) against obtained by screening the were expressed HEK293F cells, fusing immunoglobulin (Ig) G1 Fc fragment (VNAR-Fc-11, VNAR-Fc-29, VNAR-Fc-20). The VNAR-Fc fusions bound to an affinity KD ranging 0.2131 ∼ 465.3 μM, ability inhibit patients' IgE binding VNAR-20 VNAR-29 displayed more stable during molecular dynamics simulation. sites are distributed conserved epitopes arginine kinase. These achievements indicate application potential allergy therapeutics.

Language: Английский

Citations

0
Role of IgG4 Antibodies in Human Health and Disease DOI Creative Commons
Lili Shi, Peng Xiong,

Minglin Yang

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(9), P. 639 - 639

Published: April 25, 2025

Immunoglobulin G4 (IgG4), a unique subclass of IgG antibodies, plays diverse roles in human health and disease. Its distinct features, such as Fab-arm exchange specific mutations, confer reduced effector functions compared to other subclasses. In health, IgG4 responses contribute immune tolerance, particularly the context allergen-specific immunotherapy (AIT), where they can mediate tolerance environmental antigens, inhibit IgE-dependent mast cell degranulation, compete with IgE for allergen binding. This helps attenuating allergic symptoms is associated increased levels IgG4. However, disease scenarios, role complex. lacks complement fixation and, thus, shows ability activate pathways, it was initially thought be protective against autoimmune diseases. emerging evidence suggests that pathology. For instance, autoantibodies antigens aggravate conditions certain disorders. some cancers, may play evasion, higher correlating poor patient survival, albeit others, its exact function remains elusive. Overall, understanding precise various physiological pathological crucial developing targeted therapeutic strategies improving outcomes.

Language: Английский

Citations

0
Origins and diversity of pan-isotype human bone marrow plasma cells DOI Creative Commons
Gaspar A. Pacheco, Vishal Rao, Duck Kyun Yoo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 10, 2024

Abstract Bone marrow plasma cells (BMPCs) produce durable, protective IgM, IgG, and IgA antibodies, in some cases, pro-allergic IgE but their properties sources are unclear. We charted single BMPC transcriptional clonal heterogeneity food-allergic non-allergic individuals across CD19 protein expression given its inverse correlation to longevity. Transcriptional diversity revealed distinct functional profiles. Additionally, distribution of somatic hypermutation intraclonal antibody sequence variance suggest that CD19low CD19high BMPCs arise from recalled memory germinal center B cells, respectively. Most were peanut-allergic individuals; two out 32 independent donors bound peanut antigens vitro vivo. These findings shed light on origins highlight the bone as a source pathogenic allergy.

Language: Английский

Citations

3
B cell memory of Immunoglobulin E (IgE) antibody responses in allergy DOI
Weslley Fernandes‐Braga, Maria A. Curotto de Lafaille

Current Opinion in Immunology, Journal Year: 2024, Volume and Issue: 91, P. 102488 - 102488

Published: Sept. 27, 2024

Language: Английский

Citations

2
Leaping toward Tolerance DOI

Sarita U. Patil,

Stephanie K. Dougan, Michael Dougan

et al.

NEJM Evidence, Journal Year: 2024, Volume and Issue: 3(6)

Published: May 28, 2024

The LEAP (Learning Early About Peanut allergy) trial established that early introduction of peanuts in high-risk infants prevents the childhood development peanut allergy for most.1 LEAP-Trio, a 12-year follow up participants, published this issue NEJM Evidence, demonstrates long-term protection from allergy.2 In comparison with 80% reduction LEAP, LEAP-Trio found 75% consumption group compared avoidance group, despite ad libitum children who were originally peanut-tolerant.2 Clinically, results long-lived tolerance.

Language: Английский

Citations

0
Whence and wherefore IgE? DOI
Rifat Rahman, Duane R. Wesemann

Immunological Reviews, Journal Year: 2024, Volume and Issue: 326(1), P. 48 - 65

Published: July 23, 2024

Summary Despite the near ubiquitous presence of Ig‐based antibodies in vertebrates, IgE is unique to mammals. How and why it emerged remains mysterious. expression greatly constrained compared other IgH isotypes. While isotypes are relatively abundant, soluble has a truncated half‐life, plasma cells mostly short‐lived. its rarity, consequential can trigger life‐threatening anaphylaxis. production reflects dynamic steady state with IgG memory B feeding short‐lived production. Emerging evidence suggests that may also potentially be produced longer‐lived as well, perhaps an aberrancy stemming from evolutionary roots antibody isotype likely functioned more like IgG. As late derivative ancient systemic system, benefits mammals stems system's adaptive recognition response capability. However, tendency for massive, systemic, long‐lived production, common IgG, were not good fit IgE. The derivation system millions years was at antigen de‐sensitization now functioning highly specialized antigen‐sensitization function required heavy restrictions on production—insufficiency which contribute allergic disease.

Language: Английский

Citations

0
The Potential of Human Monoclonal IgE Antibodies to Establish Biological Potency and Stability of Allergen Extracts DOI Creative Commons
Ronald L. Rabin

Current Allergy and Asthma Reports, Journal Year: 2024, Volume and Issue: 24(9), P. 471 - 475

Published: July 24, 2024

Abstract Purpose of Review Allergenic extracts are often standardized to control for potency, either by measuring concentrations major allergens or “overall allergenicity” competition IgE in pooled sera from highly allergic subjects with a reference extract. Recent developments present an opportunity use human mAb cloned define potency allergenic extracts. Findings Two recent revising measurements allergen extracts: cloning specific and extensive epitope mapping proteins. Summary Because recognize biologically relevant epitopes, they novel determine the potencies may contribute science base standardization.

Language: Английский

Citations

0
T follicular helper cells in food allergy DOI
Jennifer Chen, Dong‐Uk Lee,

Uthaman Gowthaman

et al.

Current Opinion in Immunology, Journal Year: 2024, Volume and Issue: 91, P. 102461 - 102461

Published: Sept. 14, 2024

Language: Английский

Citations

0
Influence du sexe sur l’épidémiologie, la sévérité et la réponse au traitement de l’allergie alimentaire DOI

C. Elbany,

Philippe Bégin

Revue française d'allergologie, Journal Year: 2024, Volume and Issue: 65(1), P. 104159 - 104159

Published: Oct. 16, 2024

Citations

0
Evaluating the Pathogenic Potential of IgE Targeting Cross-Reactive Carbohydrate Determinants in Dogs DOI Creative Commons
Thierry Olivry, Ana Mas‐Fontao, Laura Widorn

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(22), P. 3275 - 3275

Published: Nov. 14, 2024

Cross-reactive carbohydrate determinants (CCDs) are complex N-glycans shared among allergens of plant, insect venom, and nematode origin. In allergic humans, IgE anti-CCD often develop cause discrepancies between serological skin tests. Overall, CCD-IgE believed to be low pathogenic relevance. IgE-targeting CCDs also detected in companion animals, but their potential biological relevance unknown. Herein, we first establish that, 34 dogs with atopic dermatitis, the presence serum was 14 pets (41.2%). dogs, as heterogeneous, they differentially recognized four distinct CCD-expressing proteins. The associated a higher more frequent recognition plant extracts not intradermal Two different proteins did elicit immediate reactions when injected intradermally detectable IgE. Similarly, two induce activation mast cells passively transferred canine Altogether, these results suggest that likely have little blocking them allergen-specific tests is warranted avoid false-positive extracts.

Language: Английский

Citations

0