Use of FISH‐FLOW as a Method for the Identification and Quantification of Bacterial Populations

Molecular Nutrition & Food Research, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Abstract The gastrointestinal tract (GIT) harbors the largest group of microbiotas among microbial communities human host. resident organisms typical a healthy gut are well adapted to environment while alteration these populations can trigger disorders that may affect health and well‐being Various investigations have applied different tools study bacterial in their correlation with such as inflammatory bowel disease (IBD), obesity, diabetes. This proposes fluorescent situ hybridization, combined flow cytometry (FISH‐FLOW), an alternative approach for phylum level identification Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria quantification target bacteria from GIT based on analysis fecal samples, where results validated by quantitative polymerase chain reaction (qPCR) 16S ribosomal ribonucleic acid (16s rRNA) sequencing. obtained via FISH‐FLOW experimental show high specificity developed probes hybridization bacteria. study, therefore, suggests reliable method studying correlating those metagenomic same samples.

Language: Английский

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Depression: The Vagus Nerve and Gut-Brain Axis

Published: Jan. 1, 2024

Language: Английский

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