SNP-Associated Differential Methylation in ARHGEF38: Insights into Genetic-Epigenetic Interactions DOI Open Access

Emese H.C. Kovács,

Lucas G. Casten, Niamh Mullins

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Objective Associations have been seen between suicidal behavior and differential DNA methylation of certain genes, with one study showing significant hypomethylation ARHGEF38 in postmortem brain samples from individuals bipolar disorder who died by suicide. Our objective was to explore a history suicide attempt. Method With pyrosequencing, we looked at the previously identified region interest ARHGEF38. We investigated levels 3 CpG sites 47 attempt, without non-bipolar controls. Results None measured had an association groups, although there were distinct clusters each group. Applying genotypes SNPs found interest, rs2121558 rs1447093, these showed stepwise site, regardless phenotype. Conclusions In this relatively small sample size study, not associated failing replicate findings related outcome, death. However, did provide evidence SNP interplay region. This highlights potential relevance considering genetics when interrogating epigenetic mechanisms. Highlights is attempt Methylation heavily influenced presence Suicide phenotype, genetics, type impact

Language: Английский

SNP-Associated Differential Methylation in ARHGEF38: Insights into Genetic-Epigenetic Interactions DOI Open Access

Emese H.C. Kovács,

Lucas G. Casten, Niamh Mullins

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Objective Associations have been seen between suicidal behavior and differential DNA methylation of certain genes, with one study showing significant hypomethylation ARHGEF38 in postmortem brain samples from individuals bipolar disorder who died by suicide. Our objective was to explore a history suicide attempt. Method With pyrosequencing, we looked at the previously identified region interest ARHGEF38. We investigated levels 3 CpG sites 47 attempt, without non-bipolar controls. Results None measured had an association groups, although there were distinct clusters each group. Applying genotypes SNPs found interest, rs2121558 rs1447093, these showed stepwise site, regardless phenotype. Conclusions In this relatively small sample size study, not associated failing replicate findings related outcome, death. However, did provide evidence SNP interplay region. This highlights potential relevance considering genetics when interrogating epigenetic mechanisms. Highlights is attempt Methylation heavily influenced presence Suicide phenotype, genetics, type impact

Language: Английский

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