NOVEOS and ImmunoCAP Have Similar Performances for Diagnosing Food Allergies

The Journal of Allergy and Clinical Immunology In Practice, Journal Year: 2024, Volume and Issue: 12(6), P. 1605 - 1613.e5

Published: March 6, 2024

BackgroundThe clinical significance of newly available platforms for specific IgE measurement must be evaluated. However, data are lacking NOVEOS (Hycor), especially food allergens.ObjectiveWe compared the technical and performance two (ImmunoCAP NOVEOS) to measure 10 allergens.MethodsSera from 289 clinically characterized patients were tested six allergen extracts (egg white, cow's milk, peanut, hazelnut, fish, shrimp) four molecular allergens (Gal d 1, Bos 8, Ara h 2, Cor a 14). Specific measurements carried out using ImmunoCAP methods. Food allergy diagnoses established according international guidelines.ResultsA strong correlation (ρ > 0.9) was present between whereas concentrations measured with consistently lower (mean, –15%) than ImmunoCAP. provided similar overall odds ratios relative risks diagnosis both allergens. When all considered, better receiver operating characteristic curves (P = .04). Finally, we found that most discordant results observed hazelnut peanut related cross-reactive carbohydrate determinants these ImmunoCAP.ConclusionsSpecific determination by either or (odds allergy, 25.1 33.0, respectively) is highly informative regarding risk in selected population. The platform presents advantage being less affected unwanted reactivity owing determinant-specific while requiring 10-fold test sample volume.

Language: Английский

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Molecular and Immunological Characterization of Troponin C: An Allergen from Scylla paramamosain

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Scylla paramamosain, a crustacean of substantial importance, is frequent trigger food allergies. This study examined the molecular and immunological properties troponin C from S. paramamosain (Scy p TnC) as an allergen. The findings indicated that thermal stability Scy TnC comprised 150 amino acids facilitated induction CD63/CD203c in basophils crab allergy patients. Furthermore, treatment with chemical denaturants caused structural degradation, which resulted diminished IgG binding capacity. Subsequently, 6 linear epitopes 4 conformational epitope regions were predicted, at C-terminal being conserved throughout 9 discovered TnCs. Concurrently, mice sensitized exhibited markedly increased levels IgE IL-4 release, provoking Th2 immune response. results reveal allergens enhance existing knowledge regarding allergenic components crabs, thereby facilitating advancement diagnostics targeted therapeutic strategies.

Language: Английский

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Clinical Pharmacognosy

Published: March 13, 2025

Two distinct and important fields of pharmacy are introduced in this chapter, "Clinical Pharmacognosy," along with a novel integrated multidisciplinary characteristic between the two subjects pharmacognosy clinical pharmacy. Clinical focuses on herbal medications that may interact other have either positive or negative effects. Monitoring is essential to reduce drug reactions improve safety remedies. pharmacists play an role managing harmful effects interactions drugs. Phyto equivalency one techniques used for tracking effectiveness medications, which required. This topic includes detailed discussion pharmacognosy, its application healthcare, herbal-drug interactions, phytoequivalence.

Language: Английский

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Enhancing production and assessing IgE reactivity of dog allergen Can f 6 in Pichia pastoris and Escherichia coli

Applied Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 109(1)

Published: March 29, 2025

Abstract Pet allergies are increasingly prevalent in developed nations, significantly affecting humans and strongly linked with asthma rhinitis. Allergic reactions to cats dogs affect 15.7% of Americans 27.2% Europeans, sensitization rates dog allergens reaching 56.0% Denmark. Despite these concerns, ownership remains widespread, 25% European 45.5% US households owning at least one dog. With on the rise current diagnostic therapeutic approaches predominantly relying inherently inconsistent allergen extracts derived from natural sources, recombinant production offers a pathway component-resolved diagnostics, improving specificity reliability allergy diagnosis. The present research explored, for first time, component glycoprotein Can f 6 eukaryotic expression system Pichia pastoris compared its IgE antigenicity (rCan 6) variants produced Escherichia coli . Yields were increased by fusing maltose binding protein (MBP), resulting 1.8-fold increase E. threefold P. Antigenicity analysis showed that N-glycosylation is not critical folding or recognition 6, making both systems equally suitable producing allergen. Notably, -produced MBP fused purified through cation exchange chromatography yielded lower quantity. Still, it exhibited stronger reactivity than same using anion chromatography. Graphical abstract

Language: Английский

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Allergen chip in molecular diagnostic: from cohort analysis to personalized patient care

Russian Journal of Biotherapy, Journal Year: 2025, Volume and Issue: 24(1), P. 10 - 26

Published: April 15, 2025

Background . The allergic diseases are growing everywhere. exact diagnosis of allergens becomes more and critical because it significantly affects the further treatment patient, including question about using allergen-specific immunotherapy. There several laboratory instrumental tests among which skin most available. Despite its low cost, this method is characterized by a large percentage false-positive and, importantly, false-negative results. In addition, that can be detected limited. Aim. To conduct comparative analysis effectiveness diagnostic accuracy allergen chips from different manufacturers to evaluate their applicability in molecular diseases. Results ImmunoCAP ISAC Allergy Explorer (ALEX) gradually occupying niche Both methods have benefits disadvantages. high cost difficulties supplying foreign materials sharply limit widespread use allergy profiles Russia. On other hand, personalized medicine requires doctors patient-centered care, completed existing tools. panels for various regions Russia solve problem. Identifying common clinically significant molecules each region will enable accurate allergies patient treatment. This improve medical care quality increase therapy effectiveness. Conclusion. Developing implementing new creating optimal important steps towards improving These measures help clinicians accurately identify relevant develop individual plans ultimately leading better public health lower economic costs treating allergies.

Language: Английский

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