ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(21), P. 27714 - 27727

Published: May 8, 2024

Rapid and accurate quantification of metabolites in different bodily fluids is crucial for a precise health evaluation. However, conventional metabolite sensing methods, confined to centralized laboratory settings, suffer from time-consuming processes, complex procedures, costly instrumentation. Introducing the MXene/nitrogen-doped electrochemically exfoliated graphene (MXene@N-EEG) nanocomposite as novel biosensing platform this work addresses challenges associated with leveraging concept molecularly imprinted polymers (MIP) enables highly sensitive, specific, reliable detection metabolites. To validate our technology, we utilize agmatine significant biologically active metabolite. The MIP biosensor incorporates electrodeposited Prussian blue nanoparticles redox probe, facilitating direct electrical signaling binding polymeric matrix. MXene@N-EEG nanocomposite, excellent metal conductivity large electroactive specific surface area, effectively stabilizes nanoparticles. Furthermore, increasing content agmatine-imprinted cavities on electrode enhances sensitivity biosensor. Evaluation designed buffer solution plasma samples reveals wide linear concentration range 1.0 nM–100.0 μM (R2 = 0.9934) limit 0.1 nM. Notably, developed microfluidic offers low cost, rapid response time target molecule (10 min sample incubation), good recovery results detecting samples, acceptable autonomous performance on-chip detection. Moreover, its high reliability position MIP-based promising candidate miniaturized devices potential scalable production point-of-care applications.

Language: Английский