Nitric oxide facilitates the S‐nitrosylation and deubiquitination of Notch1 protein to maintain cancer stem cells in human NSCLC DOI Creative Commons
Tenglong Zhang, Jiaxin Lei, Ming Zheng

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(21)

Published: Nov. 1, 2024

Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality, with tumour heterogeneity, fueled by stem cells (CSCs), intricately linked to treatment resistance. Therefore, it imperative advance therapeutic strategies targeting CSCs in NSCLC. In this study, we utilized RNA sequencing investigate metabolic pathway alterations NSCLC and identified a crucial role nitric oxide (NO) metabolism governing CSC stemness, primarily through modulation Notch1 protein. Mechanistically, NO-induced S-nitrosylation facilitated its interaction deubiquitylase UCHL1, increased protein stability enhanced stemness. By inhibiting NO synthesis downregulating UCHL1 expression, validated impact on Notch signalling Importantly, effectively reduced populations within patient-derived organoids (PDOs) during radiotherapy. This mechanism presents promising target surmount radiotherapy resistance treatment.

Language: Английский

Nanoparticles of Cerium-Doped Zeolitic Imidazolate Framework-8 Promote Soft Tissue Integration by Reprogramming the Metabolic Pathways of Macrophages DOI Creative Commons

Qiqian Yan,

Shanwei Shi,

Ge Yang

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(7), P. 4241 - 4254

Published: June 8, 2023

Soft tissue integration around the abutment of implants is basis long-term retention implants. Macrophages are an important component involved in repair soft due to their crucial role improving biological structure connective tissues by regulating fiber synthesis, adhesion, and contraction gingival fibroblasts. Recent studies have illustrated that cerium-doped zeolitic imidazolate framework-8 (Ce@ZIF-8) nanoparticles (NPs) can attenuate periodontitis via both antibacterial anti-inflammatory effects. However, effect Ce@ZIF-8 NPs on unknown. Herein, we first prepared a one-pot synthesis. Then, probed regulatory macrophage polarization, further experiments were performed study changes synthesis as well adhesion fibroblasts M2 environment stimulated NPs. Strikingly, be internalized M1 macrophages through macropinocytosis caveolae-mediated endocytosis addition phagocytosis. By catalyzing hydrogen peroxide produce oxygen, mitochondrial function was remedied, while hypoxia inducible factor-1α restrained. shifted from phenotype this metabolic reprogramming pathway, provoking integration. These results provide innovative insights into facilitating

Language: Английский

Citations

2
INOS ablation promotes corneal wound healing via activation of Akt signaling DOI
Ziwen Sun, Kunpeng Lu,

Qing He

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 243, P. 109886 - 109886

Published: April 5, 2024

Language: Английский

Citations

0
Exploring the role of iNOS in HFpEF-Related myocardial fibrosis: Involvement of PTEN-PI3K/AKT signaling pathway DOI
Hongjun You,

Qiling Gou,

Mengya Dong

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 734, P. 150589 - 150589

Published: Aug. 23, 2024

Language: Английский

Citations

0
Charnolosome nanotheranostics in acute ischemic stroke and traumatic brain injury DOI

Sushil Sharma

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 569 - 581

Published: Oct. 4, 2024

Language: Английский

Citations

0
Nitric oxide facilitates the S‐nitrosylation and deubiquitination of Notch1 protein to maintain cancer stem cells in human NSCLC DOI Creative Commons
Tenglong Zhang, Jiaxin Lei, Ming Zheng

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2024, Volume and Issue: 28(21)

Published: Nov. 1, 2024

Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality, with tumour heterogeneity, fueled by stem cells (CSCs), intricately linked to treatment resistance. Therefore, it imperative advance therapeutic strategies targeting CSCs in NSCLC. In this study, we utilized RNA sequencing investigate metabolic pathway alterations NSCLC and identified a crucial role nitric oxide (NO) metabolism governing CSC stemness, primarily through modulation Notch1 protein. Mechanistically, NO-induced S-nitrosylation facilitated its interaction deubiquitylase UCHL1, increased protein stability enhanced stemness. By inhibiting NO synthesis downregulating UCHL1 expression, validated impact on Notch signalling Importantly, effectively reduced populations within patient-derived organoids (PDOs) during radiotherapy. This mechanism presents promising target surmount radiotherapy resistance treatment.

Language: Английский

Citations

0