RELT Is Upregulated in Breast Cancer and Induces Death in Breast Cancer Cells DOI Creative Commons

Maryann Batiste,

Bethany Joy,

Cindy J. Yee

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2667 - 2667

Published: Nov. 22, 2024

Receptor Expressed in Lymphoid Tissues (RELT) is a TNFRSF member that has two paralogs, RELL1 and RELL2; the three proteins are collectively referred to as RELT family members (RELTfms). We sought evaluate expression cancerous cells by using real-time PCR, western blotting, flow cytometry, immunohistochemistry (IHC). The mechanism of RELT-induced cell death was assessed luciferase assays, morphology staining. localization detected through immunofluorescence co-immunoprecipitation used test whether mutated interacts with OXSR1 kinase. protein significantly elevated lines representing breast lung cancer, whereas RELL2 relatively consistent across different lines. surface highest monocytes. IHC staining revealed increased malignant cancer biopsies compared patient-matched benign tissue. RELTfm overexpression induced MDA-MB-231 (231) cells, accompanied phosphatidylserine externalization Caspase-3/7 activation. co-transfection plasmids predicted block phosphorylation kinase did not abrogate apoptosis, indicating activation p38 required for death. Interestingly, nuclear 231 HEK-293 cells. These results demonstrate induces an apoptotic pathway does require possesses ability translocate nucleus, novel finding warrants further investigation.

Language: Английский

The RELT Family of Proteins: An Increasing Awareness of Their Importance for Cancer, the Immune System, and Development DOI Creative Commons
John Cusick,

Jessa Alcaide,

Yihui Shi

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(10), P. 2695 - 2695

Published: Oct. 2, 2023

This review highlights Receptor Expressed in Lymphoid Tissues (RELT), a Tumor Necrosis Factor Superfamily member, and its two paralogs, RELL1 RELL2. Collectively, these three proteins are referred to as RELTfms have gained much interest recent years due their association with cancer other human diseases. A thorough knowledge of physiological functions, including the ligand for RELT, is lacking, yet emerging evidence implicates variety processes cytokine signaling pathways that either promote cell death or survival. T cells from mice lacking RELT exhibit increased responses against tumors inflammatory production, multiple lines indicate may an immunosuppressive environment tumors. The relationship individual different cancers not universal however, indicates be risk factors certain appear protective cancers. important additional related health microbial pathogenesis, inflammation, behavior, reproduction, development. All been strongly conserved all vertebrates, this aims provide clearer understanding current regarding interesting proteins.

Language: Английский

Citations

5
Identification and validation of a costimulatory molecule-related signature to predict the prognosis for uveal melanoma patients DOI Creative Commons

Minyao Zhao,

Yue Yu,

Zhengyu Song

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 21, 2024

Abstract Uveal melanoma (UVM) is the most common primary tumor in adult human eyes. Costimulatory molecules (CMs) are important maintaining T cell biological functions and regulating immune responses. To investigate role of CMs UVM exploit prognostic signature by bioinformatics analysis. This study aimed to identify validate a associated its progression prognosis UVM. The expression profile data training cohort validation were downloaded from Cancer Genome Atlas (TCGA) dataset Gene Expression Omnibus (GEO) dataset. 60 CM genes identified, 34 with univariate Cox regression. A was established six genes. Further, high- low-risk groups divided median, Kaplan–Meier (K-M) curves indicated that high-risk patients presented poorer prognosis. We analyzed correlation gender, age, stage, risk score on multivariate regression found only factor for Through integration microenvironment (TIME), it group more infiltration checkpoints obtained higher scores. Enrichment analysis two revealed differential parts mainly related cell–cell adhesion, regulation T-cell activation, cytokine–cytokine receptor interaction. No differences mutation burden (TMB) between groups. GNA11 BAP1 have frequencies patients. Finally, based Genomics Drug Sensitivity 2 (GDSC2) dataset, drug sensitivity may be potential beneficiaries treatment crizotinib or temozolomide. Taken together, our CM-related reliable biomarker provide ideas future treatments disease.

Language: Английский

Citations

1
SOX9 Expression Is Increased in Alzheimer’s Disease (AD) and Is Associated With Disease Progression and APOE4 Genotype: A Computational Approach DOI Open Access
Aliaa A. Alamoudi

Cureus, Journal Year: 2023, Volume and Issue: unknown

Published: March 14, 2023

Alzheimer's disease (AD) is a neurodegenerative characterized by depositions of amyloid-β protein leading to neuronal loss. Despite our understanding the several gaps remain, including role astrocytes and astrocytic genes in development progression. Recently, some reports have suggested that SOX9 transcription factor (TF), an important mediator astrocyte differentiation maturation, might be linked AD. Using human AD publicly available dataset, we aimed analyze expression its relation disease.

Language: Английский

Citations

3
Recombinant human protein TCFL5-activated NRSN2-AS1 promotes esophageal cancer progression via the microRNA-874-5p/RELT regulatory axis DOI
Wenjian Yao, Jian Liu,

Zhaoyao Hou

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 133814 - 133814

Published: July 10, 2024

Language: Английский

Citations

0
PRKN-mediated the ubiquitination of IQGAP3 regulates cell growth, metastasis and ferroptosis in early-onset colorectal cancer DOI

Gun Chen,

Linghua Cong,

Chijiang Gu

et al.

Journal of Bioenergetics and Biomembranes, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Language: Английский

Citations

0
RELT Is Upregulated in Breast Cancer and Induces Death in Breast Cancer Cells DOI Creative Commons

Maryann Batiste,

Bethany Joy,

Cindy J. Yee

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2667 - 2667

Published: Nov. 22, 2024

Receptor Expressed in Lymphoid Tissues (RELT) is a TNFRSF member that has two paralogs, RELL1 and RELL2; the three proteins are collectively referred to as RELT family members (RELTfms). We sought evaluate expression cancerous cells by using real-time PCR, western blotting, flow cytometry, immunohistochemistry (IHC). The mechanism of RELT-induced cell death was assessed luciferase assays, morphology staining. localization detected through immunofluorescence co-immunoprecipitation used test whether mutated interacts with OXSR1 kinase. protein significantly elevated lines representing breast lung cancer, whereas RELL2 relatively consistent across different lines. surface highest monocytes. IHC staining revealed increased malignant cancer biopsies compared patient-matched benign tissue. RELTfm overexpression induced MDA-MB-231 (231) cells, accompanied phosphatidylserine externalization Caspase-3/7 activation. co-transfection plasmids predicted block phosphorylation kinase did not abrogate apoptosis, indicating activation p38 required for death. Interestingly, nuclear 231 HEK-293 cells. These results demonstrate induces an apoptotic pathway does require possesses ability translocate nucleus, novel finding warrants further investigation.

Language: Английский

Citations

0