The RNA-binding protein LARP6 regulates the alternative splicing of related genes in MDA-MB-231 cells

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 6, 2025

Triple-negative breast cancer (TNBC) has the highest mortality rate of all subtypes and currently lacks effective targeted therapies. LARP6 is an RNA-binding protein associated with promotion, but its mechanism action in TNBC remains unclear. We conducted RNA sequencing (RNA-seq) improved immunoprecipitation (iRIP-seq) to identify differentially expressed genes (DEGs) alternative splice sites bound regulated by MDA-MB-231 cells. Finally, both RT-qPCR RIP-qPCR were employed for verification. Our study revealed that overexpression altered expression levels 171 number splicing events (RASEs) exceeded 1000. The (RASGs) corresponding RASEs enriched biological processes such as DNA repair, cell cycle, cellular response damage stimulus. In addition, we found tends bind CGACGAG motif. intersection peak-related RASGs suggested can 16 regulate their (AS), thus playing important role progression. research indicated may promote proliferation invasion cells directly regulating AS related genes, providing new clues therapy TNBC.

Language: Английский

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Prediction of neoadjuvant chemotherapy efficacy and prognostic biomarker analysis in patients with triple-negative breast cancer

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Background Neoadjuvant chemotherapy has become a common and effective treatment modality for triple-negative breast cancer (TNBC). The primary goal is to reduce the size of tumor, enabling breast-conserving surgery, axillary preservation, transition operability, thereby providing patients with more therapeutic options. Although neoadjuvant (NAC) demonstrated favorable outcomes in clinical practice, predicting its efficacy prognostic value TNBC remains key challenge current research. Methods This study included 248 who received NAC at two centers. By employing modeling validation approach, we aim explore predictors potential biomarkers associated NAC. Results In multivariable analysis training set, factors pathological complete response (pCR) include high biopsy-sTILs expression, biopsy-Ki67 > 20%, positive expression biopsy-androgen receptor (AR). disease-free survival (DFS) are ypN3, postoperative sTIL receipt radiotherapy, overall (OS) ypN2, Ki67 C-indices sets prediction pCR using nomogram were 0.729 0.816, respectively. DFS 0.895 0.865, OS 0.899 0.860, Conclusion established validated model pCR, DFS, undergoing demonstrates good discrimination accuracy.

Language: Английский

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Musashi inhibitor Ro 08–2750 attenuates triple-negative breast cancer cell proliferation and migration and acts as a novel chemo- and radiosensitizer

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 186, P. 118002 - 118002

Published: March 22, 2025

Language: Английский

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CircATP5C1 promotes triple-negative breast cancer progression by binding IGF2BP2 to modulate CSF-1 secretion

Cancer Biology & Therapy, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 2, 2025

Triple-negative breast cancer (TNBC) is a common malignant disease among females and severely threatens the health of women worldwide. Nowadays, circular RNAs (circRNAs) aroused our interest for their functions in human cancers, including TNBC. However, mechanism most circRNAs progression TNBC remains unclear. We found novel circRNA named circATP5C1, whose function uncovered. Tissue microarray was used to analyze association between expression circATP5C1 prognoses patients. Gain-and loss-of-function experiments were performed validate biological different cell lines. RNA-seq analyses conducted find out target genes regulated by circATP5C1. RNA pull-down assay mass spectrometry select proteins associated with FISH-immunofluorescence immunoprecipitation (RIP) complemented interaction its binding protein. CircATP5C1 identified have predictive prognosis advanced cells. Mechanistically, Colony stimulating factor 1 (CSF-1) vital downstream gene The alteration CSF-1 level validated due insulin-like growth 2 mRNA protein (IGF2BP2). Rescue demonstrated that accelerates partly via IGF2BP2 increase secretion CSF-1. This study uncovers circATP5C1/IGF2BP2/CSF-1 pathway regulating

Language: Английский

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A comprehensive review on targeted therapies for triple negative breast cancer: an evidence-based treatment guideline

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 17, 2025

Abstract Triple-negative breast cancer (TNBC) is an aggressive malignancy characterized by limited therapeutic options and poor prognosis. Despite advancements in precision oncology, conventional chemotherapy remains the cornerstone of TNBC treatment, often accompanied debilitating side effects suboptimal outcomes. This review presents a comprehensive analysis clinical trials on targeted therapies, aiming to establish novel, evidence-based treatment strategy exclusively leveraging molecularly agents. By integrating patient-specific genetic profiles with responses observed across various trial phases, this approach seeks optimize efficacy while minimizing toxicity. The proposed therapy combinations hold significant potential revolutionize offering paradigm shift toward medicine improved patient

Language: Английский

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