Succinate exacerbates mastitis in mice via gut microbiota-derived extracellular vesicles DOI Creative Commons

Min Qiu,

Yubo Hu, Chen‐Yu Zou

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 16, 2023

Abstract Background Ecological imbalance of the gut microbiota may be an important endogenous trigger mastitis, but underlying mechanisms are unclear. Recent findings indicated that selected bacteria maternal gastrointestinal can reach mammary gland through entero-mammary pathway. In addition, metabolic disturbances one most prominent ways in which dysbiosis promote distal organ diseases. Results present study, we found Gastrointestinal-associated mastitis showed significant changes levels, among succinate was significantly increased. treatment exacerbates endotoxemia-induced mice. Meanwhile, induced intestinal ecological dysregulation. Further experiments demonstrated increased production extracellular vesicles containing microbial (mEVs) lipopolysaccharides, could diffuse across damaged barrier into glands. mEVs promotes inflammation mainly activation TLR4/NF-κB Conclusions Our suggest proliferatio enteric pathogens and production, suggesting a potential strategy for intervention based on regulation pathogen inhibition. The role interspecific communication has also been elucidated.

Language: Английский

Anti-Inflammatory Properties of the Citrus Flavonoid Diosmetin: An Updated Review of Experimental Models DOI Creative Commons
Yangyang Fang, Wei Xiang, Jinwei Cui

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(7), P. 1521 - 1521

Published: March 28, 2024

Inflammation is an essential contributor to various human diseases. Diosmetin (3′,5,7-trihydroxy-4′-methoxyflavone), a citrus flavonoid, can be used as anti-inflammatory agent. All the information in this article was collected from research papers online scientific databases such PubMed and Web of Science. These studies have demonstrated that diosmetin slow down progression inflammation by inhibiting production inflammatory mediators through modulating related pathways, predominantly nuclear factor-κB (NF-κB) signaling pathway. In review, we discuss properties cellular animal models diseases for first time. We identified some deficiencies current offer suggestions further advancement. conclusion, accumulating evidence so far suggests very important role treatment disorders it candidate worthy in-depth investigation.

Language: Английский

Citations

2
Gut microbiota in perioperative neurocognitive disorders: current evidence and future directions DOI Creative Commons

Yan Sun,

Kexin Wang,

Weiliang Zhao

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: May 4, 2023

Perioperative neurocognitive disorders (PND) is a common surgical anesthesia complication characterized by impairment of memory, attention, language understanding and social ability, which can lead to decline in the quality life patients, prolong hospitalization period increase mortality rate. PND has high incidence rate, great impact on postoperative recovery caused heavy economic burden society families. In recent years, become an important public health problem. The risk population more prone gut microbiota imbalance, may also affect inflammatory response central nervous system through microbiota-gut-brain axis. Meanwhile, Neuroinflammation immune activation are mechanisms PND. Regulating probiotics or fecal bacteria transplantation significantly reduce neuroinflammation, abnormal prevent occurrence This review summarizes research progress PND, providing basis for prevention treatment

Language: Английский

Citations

6
Enhanced protein‐metabolite correlation analysis: To investigate the association between Staphylococcus aureus mastitis and metabolic immune pathways DOI
Kuo Chen,

Binhong Hu,

Jingyuan Ren

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(7)

Published: April 3, 2024

Abstract Mastitis is a disease characterized by congestion, swelling, and inflammation of the mammary gland usually caused infection with pathogenic microorganisms. Furthermore, development mastitis closely linked to exogenous pathway gastrointestinal tract. However, regulatory mechanisms governing gut‐metabolism‐mammary axis remain incompletely understood. The present study revealed alterations in gut microbiota rats an increased abundance Proteobacteria phylum. Plasma analysis significantly higher levels L‐isoleucine cholic acid along 7‐ketodeoxycholic acid. Mammary tissue showed elevated arachidonic metabolites norlithocholic Proteomic IFIH1, Tnfaip8l2, IRGM , IRF5 rats, which suggests that triggers inflammatory response immune stress. Follistatin (Fst) progesterone receptor (Pgr) were downregulated, raising risk breast cancer. Extracellular matrix (ECM) receptors focal adhesion signaling pathways while blood–milk barrier integrity was disrupted. Analysis protein‐metabolic network regulation necroptosis, protein digestion absorption, metabolism principal involved mastitis. In short, onset leads changes metabolic profiles various biological samples, including colonic contents, plasma, tissue. Key manifestations include disturbances bile metabolism, amino metabolism. At same time, compromised promoted, thereby reducing cell glands. These findings contribute more comprehensive understanding status provide new insights into its impact on system.

Language: Английский

Citations

1
Microbiome and metabolome analyses of milk and feces from dairy cows with healthy, subclinical, and clinical mastitis DOI Creative Commons
Chenglin Zhu, Yuxuan Zhao, Falong Yang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 6, 2024

Mastitis is commonly recognized as a localized inflammatory udder disease induced by the infiltration of exogenous pathogens. In present study, our objective was to discern fecal and milk variations in both microbiota composition metabolite profiles among three distinct groups cows: healthy cows, cows with subclinical mastitis clinical mastitis. The microbial community significantly less rich diverse than one harbored cows. parallel, caused strong disturbance microbiota. Metabolomic showed that eleven twenty-eight molecules exhibited significant differences feces milk, respectively. Similarly, profile, metabolome affected more extensively metabolome, particular reference amino acids sugars. Pathway analysis revealed metabolism energy could be considered main pathways altered These findings underscore notable distinctions samples groups, from microbiome metabolomic points view. This observation stands enhance comprehension dairy

Language: Английский

Citations

1
Disturbances of the gut microbiota-derived tryptophan metabolites as key actors in vagotomy-induced mastitis in mice DOI Creative Commons
Yuhong He, Caijun Zhao,

Niri Su

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(8), P. 114585 - 114585

Published: Aug. 1, 2024

Previous studies have demonstrated that gut microbiota dysbiosis promotes the development of mastitis. The interaction vagus nerve and endows host homeostasis regulates disease development, but whether participates in pathogenesis mastitis is unclear. Here, vagotomized mice exhibit disruption blood-milk barrier mammary gland inflammation. Notably, damage caused by vagotomy are dependent on microbiota, as evidenced antibiotic treatment fecal transplantation. Vagotomy significantly alters microbial composition tryptophan metabolism reduces 5-hydroxyindole acetic acid (5-HIAA) level. Supplementation with 5-HIAA alleviates vagotomy-induced mastitis, which associated activation aryl hydrocarbon receptor (AhR) subsequent inhibition NF-κB pathway. Collectively, our findings indicate important role vagus-mediated gut-mammary axis imply a potential strategy for targeting vagus-gut interaction.

Language: Английский

Citations

1
Rumen–mammary gland axis and bacterial extracellular vesicles: Exploring a new perspective on heat stress in dairy cows DOI Creative Commons

Qi Huang,

Yang Xiao, Peng Sun

et al.

Animal nutrition, Journal Year: 2024, Volume and Issue: 19, P. 70 - 75

Published: Sept. 17, 2024

Language: Английский

Citations

1
Succinate exacerbates mastitis in mice via extracellular vesicles derived from the gut microbiota: a potential new mechanism for mastitis DOI Creative Commons

Min Qiu,

Cong Ye, Xiaotong Zhao

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 15, 2024

A high grain diet causes an ecological imbalance in the gut microbiota and serves as important endogenous trigger of mastitis dairy cows, but underlying mechanisms are unclear. Our previous study revealed that subacute rumen acidosis (SARA)-associated has distinct metabolic profiles rumen, especially a significant increase succinate, role succinate pathogenesis remains Succinate treatment exacerbates low-grade endotoxemia-induced mice. Specifically, increased production microbiota-extracellular vehicles (mEVs) containing lipopolysaccharides, which can diffuse across damaged intestinal barrier into mammary glands. Administration mEVs promotes inflammation via activation TLR4/NF-κB pathway. findings suggest through proliferation enteric pathogens production, suggesting potential strategy for intervention on basis regulation pathogen inhibition. The interspecific communication also been elucidated.

Language: Английский

Citations

1
High-grain feeding contributes to endotoxin contamination in dairy milk DOI
Siska Aditya, M. Qumar, M F Karimy

et al.

Toxicon, Journal Year: 2024, Volume and Issue: 241, P. 107659 - 107659

Published: Feb. 28, 2024

Language: Английский

Citations

0
Emerging role of ruminal microbiota in the development of perinatal bovine diseases DOI Creative Commons
Caijun Zhao, Xiaoyu Hu,

Naisheng Zhang

et al.

Animals and zoonoses., Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

As the largest and most important digestive organ of ruminant, rumen was a natural fermentation tank dairy cows, in which there were many kinds microorganisms. These microorganisms constituted symbiotic complex ecosystems, termed as ruminal microbiota, interacted with each other or cross-talked host to maintain homeostasis host. In turn, microbiota could be affected by various factors, including genetics, sex, age, diet pH. Meanwhile, dysbiosis, especially caused excessive consumption high-grain diet, associated development perinatal diseases, subacute acidosis, systemic inflammation, mastitis, endometritis, liver diseases laminitis. A better understanding how dysbiosis affects may lead novel strategies treatments for bovine diseases. Hence, purpose this review is summarize latest studies discussing composition regulatory factors highlight relationship between potential mechanisms, model studying causality improve health based on optimizing microbiota.

Language: Английский

Citations

0
Unveiling the microbial metabolites in ruminant milk for medicinal purposes DOI Creative Commons
Qianchi Jia,

Tongling Yang,

Fan Chang

et al.

Food Frontiers, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 4, 2024

Abstract Milk, as a dietary supplement, is crucial for maintaining health and potentially preventing disease throughout life. Both traditional Chinese medicine modern nutritional science have revealed that milk from different mammals can benefit humans, exhibiting distinct medicinal functions. It be inferred contains not only multiple nutrients but also an abundance of bioactive components benefits consumers. However, limited studies focused on the differences in metabolites derived various ruminants their medical Here, we present comprehensive review diversity microbial associated benefits, which are primarily shaped by rumen‐mammary gland axis. Our findings reveal pivotal role played facilitating microbiome's influence human physiology. Finally, conclude ruminant may offer path to harness these metabolites’ therapeutic benefits. To increase milk, there need design supplements dairy ruminants, could enhance milk's value aroma, revolutionizing husbandry.

Language: Английский

Citations

0