Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 1, 2024

Arthrobotrys oligospora has been utilized as a model strain to study the interaction between fungi and nematodes owing its ability capture by developing specialized traps. A previous showed that high-osmolarity glycerol (Hog1) signaling regulates osmoregulation nematocidal activity of A. oligospora. However, function downstream transcription factors Hog1 in nematode-trapping (NT) remains unclear. This aimed investigate functions potential regulatory network AoMsn2, factor pathway The AoMsn2 was characterized using targeted gene deletion, phenotypic experiments, real-time quantitative PCR, RNA sequencing, untargeted metabolomics, yeast two-hybrid analysis. Loss Aomsn2 significantly enlarged swollen hyphae, with an increase septa significant decrease nuclei. In particular, spore yield, germination rate, traps, nematode predation efficiency were remarkably decreased mutants. Phenotypic transcriptomic analyses revealed is essential for fatty acid metabolism autophagic pathways. Additionally, metabolomic analysis identified important modulation secondary metabolites. Furtherly, we analyzed protein based on Kyoto Encyclopedia Genes Genomes map online website STRING. Finally, six putative proteins Y2H Our reveals plays crucial roles growth, conidiation, trap development, metabolism, well establishes broad basis understanding mechanisms morphogenesis environmental adaptation NT fungi.

Language: Английский

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Subcellular biochemistry and biology of filamentous entomopathogenic fungi

Advances in applied microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 35 - 58

Published: Jan. 1, 2024

Language: Английский

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Alcohol dehydrogenase 1 acts as a scaffold protein in mitophagy essential for fungal pathogen adaptation to hypoxic niches within hosts

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139651 - 139651

Published: Jan. 8, 2025

Language: Английский

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The Hog1-Nmd5 signaling pathway regulates asexual development, lipid metabolism, stress response, trap morphogenesis, and secondary metabolism of Arthrobotrys oligospora

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 19, 2025

The high-osmolarity glycerol (HOG) signalling pathway, comprising Ste11/Ssk2/Ssk22 (MAPKKK), Pbs2 (MAPKK), and Hog1 (MAPK), is an important conserved pathway in fungi. However, the functions downstream regulatory factors of nematode-trapping (NT) fungi remain poorly understood. Here, three proteins (AoNmd5, AoPyp1, AoPtp) interacting with were screened a representative NT fungus Arthrobotrys oligospora using yeast screening library verified two-hybrid (Y2H) assay. function AoNmd5 was furtherly characterized by phenotypic comparison, staining technique, multi-omics analyses. essential for vegetative growth, conidial development, trap morphogenesis, nematode predation ability. In addition, played crucial roles endocytosis, lipid metabolism, reactive oxygen species, stress response, autophagy, other metabolic processes. Furthermore, we constructed interaction network based on transcriptomic analysis Y2H, revealing its significant role respiratory chain redox processes as well small GTPase Ran1, which mediates nucleocytoplasmic shuttling. These findings suggest that Hog1-Nmd5 has pleiotropic A. oligospora. This study deepens our understanding HOG importins

Language: Английский

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Lipophagy acts as a nutritional adaptation mechanism for the filamentous entomopathogenic fungus Beauveria bassiana to colonize within the hosts

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Metabolic adaptation to various nutrients is crucial for the pathogenic growth and virulence of filamentous fungal pathogens. Despite its importance, mechanisms underlying nutrient shifts, especially at subcellular level, remain incompletely understood. Our study aims investigate involved in metabolic fungi. The entomopathogenic fungus Beauveria bassiana was used as a representative Gene functional analyses were conducted via gene disruption complementation. Vacuolar targeting lipid droplets determined with transmission electron microscopy fluorescence microscopy. Protein interaction yeast-two hybridization co-immunoprecipitation methods. found initiate autophagy, further lipophagy, when transitioning from utilizing fatty acids carbohydrates, while also proliferating host hemocoel. three critical autophagy-related genes (ATG), specifically BbATG1, BbATG8, BbATG11, hindered vacuolar (LD) worsened impaired dimorphism acid medium subjected cell-wall perturbance stress. Notably, BbSun4, protein containing SUN4 domain, required it tagged droplets. BbMcp, which features methyl-accepting chemotaxis-like engaged directly both BbAtg8 thereby enhancing between these proteins. It important note that BbMcp solely facilitated lipophagy during shifts rather than starvation loss proved detrimental cytomembrane integrity, growth, overall development, ultimately leading marked reduction virulence. Lipophagy molecular pathway consists selective autophagy receptor, bridging factor, Atg8, essential colonizing within niches. This deepens our understanding mechanism fungus-host pathways autophagy.

Language: Английский

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Comparative Transcriptomics of the Entomopathogenic Fungus Beauveria bassiana Grown on Aerial Surface and in Liquid Environment

Current Microbiology, Journal Year: 2024, Volume and Issue: 81(8)

Published: July 1, 2024

Language: Английский

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