The neurodevelopmental regulatory role and clinical value of hsa-circ-CORO1C–hsa-miR-708-3p–JARID2 + LNPEP axis in early-onset schizophrenia DOI Creative Commons

Zexuan Li,

Xinzhe Du,

Xijin Wang

et al.

Schizophrenia, Journal Year: 2024, Volume and Issue: 10(1)

Published: Dec. 19, 2024

Genes discovered by previous epigenetic studies of schizophrenia have focused solely on diagnostics or pathology, potentially leading to a disconnection between them. Using these molecules identify the disease is considered insufficient. MicroRNAs (miRNAs) binding messenger RNAs (mRNAs) can lead mRNA degradation, while circular (circRNAs), miRNAs as sponge, reduce inhibitory effect mRNAs. CircRNAs, miRNAs, and mRNAs form multi-molecular axis that bind regulate expression each other, thereby affecting biological function. This study early-onset (EOS), aiming consisting circRNAs, with both neurobiological function diagnostic value assist in identification. In discovery cohort 10 drug-naïve, first-episode patients EOS matched healthy controls (HCs), differentially expressed (DE) circRNAs were identified via Illumina high-throughput sequencing. validation cohort-1 (40 v.s. 50 HCs), candidate further screened using Real-time polymerase chain reaction, Sanger sequencing, RNase R assay. Combining dual-luciferase reporter assay overexpression/knockdown experiments, circRNAs–miRNAs-mRNAs regulatory relationships has been established. Subsequently, functions genes explored through zebrafish embryo manipulation neural differentiation. The clinical entire was assessed cohort-2 (84 67 HCs). Patients exhibited profiles 487 DE 101 compared HCs. effects hsa-circ-CORO1C hsa-miR-708-3p, hsa-miR-708-3p target JARID2 LNPEP elucidated. Among them, caused aberrant phenotypes including significant craniocerebral malformation impaired neuron axon growth. could facilitate neuronal differentiation augment synaptic formation. addition their functions, combined efficacy whole axis, where serve sponge for alleviate its suppressive LNPEP, surpassed any individual gene we found EOS. Our demonstrated hsa-circ-CORO1C–hsa-miR-708-3p–JARID2 + first time. By integrating evidence from genetic, neurophenotypic, perspectives, expanded comprehension pathological mechanism provided reference identifying reliable objective biomarkers

Language: Английский

The role of mTORC1/TFEB axis mediated lysosomal biogenesis and autophagy impairment in fluoride neurotoxicity and the intervention effects of resveratrol DOI
Huayang Tang,

Haoqi Hou,

Song Li

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 467, P. 133634 - 133634

Published: Jan. 28, 2024

Language: Английский

Citations

10
Safranal Ameliorates Renal Damage, Inflammation, and Podocyte Injury in Membranous Nephropathy via SIRT/NF-κB Signalling DOI Creative Commons

Yan Bao,

Ya-mei Ge,

Zhengtao Wang

et al.

Current Medical Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Language: Английский

Citations

1
Inflammation May Mediate the Effects of Fluoride on Liver and Kidney Function of Adults: Cross-Sectional Studies in China DOI

Minghao Geng,

Yi Cheng,

Leizhen Duan

et al.

Biological Trace Element Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Language: Английский

Citations

0
Methylated urolithin a attenuates lipopolysaccharide-induced memory impairment and behavioral disorders by inhibiting mitochondrial inflammation through the Drp1/HK1/NLRP3 pathway DOI
Peng Chen, Wenhua Xiao, Fan Zou

et al.

Journal of Functional Foods, Journal Year: 2025, Volume and Issue: 129, P. 106851 - 106851

Published: May 9, 2025

Language: Английский

Citations

0
Resveratrol Attenuates Prenatal X-ray-Induced Microcephaly and Adult Depression via SIRT1-Mediated Senescence Suppression and TPH2/5-HT Pathway Restoration in Mice DOI Creative Commons

Yu Feng Zhang,

Zhengping Xu, Chen Wang

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 143, P. 156845 - 156845

Published: May 21, 2025

With increasing use of medical imaging (e.g., CT scans) and environmental radiation sources, over 2 % pregnancies worldwide are inadvertently exposed to low-dose ionizing (IR), raising urgent concerns about fetal neuroprotection. While prenatal IR is implicated in microcephaly lifelong neuropsychiatric risks, prior studies have not resolved whether sirtuin-mediated pathways, particularly SIRT1/TPH2 signaling, drive these deficits or dietary phytochemicals like resveratrol can mitigate them. To determine (1) the role signaling IR-induced neurodevelopmental psychiatric impairments, (2) therapeutic potential maternal supplementation counteract effects-a strategy previously explored models. Mouse cohorts received X-ray irradiation (0, 1.0 Gy, Gy gestational day 8) with/without supplementation, followed by longitudinal cortical behavioral analyses. RNA sequencing/Western blotting quantified SIRT1, TPH2, BDNF, senescence markers (P16, P21 SA-β-gal). 5-HT levels were assessed ELISA. Depression-like behaviors tested via forced swim tail suspension. IR-exposed fetuses exhibited progressive with reduced thickness, accompanied SIRT1 downregulation, BDNF suppression, elevated cellular senescence. Adult offspring displayed depression-like behaviors, linked TPH2 downregulation diminished levels. Resveratrol normalized restored neurotrophic factors, attenuated both depressive phenotypes. This study provides first evidence that a central mediator prevents damage depression rescuing this pathway. These findings position as novel, mechanism-driven intervention for neuroprotection against radiation.

Language: Английский

Citations

0
Fluoride: a double-edged sword in public health and environmental toxicology DOI

Maísa Rizzotto Sotili,

Tatiana Staudt,

Diênifer Tramontina

et al.

Environmental Monitoring and Assessment, Journal Year: 2025, Volume and Issue: 197(7)

Published: June 2, 2025

Language: Английский

Citations

0
MicroRNAs in fluorosis pathogenesis: impact on dental, skeletal, and soft tissues DOI
Suryaa Manoharan,

Syed Saadullah Ashfaq,

Ekambaram Perumal

et al.

Archives of Toxicology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 13, 2024

Language: Английский

Citations

1
The neurodevelopmental regulatory role and clinical value of hsa-circ-CORO1C–hsa-miR-708-3p–JARID2 + LNPEP axis in early-onset schizophrenia DOI Creative Commons

Zexuan Li,

Xinzhe Du,

Xijin Wang

et al.

Schizophrenia, Journal Year: 2024, Volume and Issue: 10(1)

Published: Dec. 19, 2024

Genes discovered by previous epigenetic studies of schizophrenia have focused solely on diagnostics or pathology, potentially leading to a disconnection between them. Using these molecules identify the disease is considered insufficient. MicroRNAs (miRNAs) binding messenger RNAs (mRNAs) can lead mRNA degradation, while circular (circRNAs), miRNAs as sponge, reduce inhibitory effect mRNAs. CircRNAs, miRNAs, and mRNAs form multi-molecular axis that bind regulate expression each other, thereby affecting biological function. This study early-onset (EOS), aiming consisting circRNAs, with both neurobiological function diagnostic value assist in identification. In discovery cohort 10 drug-naïve, first-episode patients EOS matched healthy controls (HCs), differentially expressed (DE) circRNAs were identified via Illumina high-throughput sequencing. validation cohort-1 (40 v.s. 50 HCs), candidate further screened using Real-time polymerase chain reaction, Sanger sequencing, RNase R assay. Combining dual-luciferase reporter assay overexpression/knockdown experiments, circRNAs–miRNAs-mRNAs regulatory relationships has been established. Subsequently, functions genes explored through zebrafish embryo manipulation neural differentiation. The clinical entire was assessed cohort-2 (84 67 HCs). Patients exhibited profiles 487 DE 101 compared HCs. effects hsa-circ-CORO1C hsa-miR-708-3p, hsa-miR-708-3p target JARID2 LNPEP elucidated. Among them, caused aberrant phenotypes including significant craniocerebral malformation impaired neuron axon growth. could facilitate neuronal differentiation augment synaptic formation. addition their functions, combined efficacy whole axis, where serve sponge for alleviate its suppressive LNPEP, surpassed any individual gene we found EOS. Our demonstrated hsa-circ-CORO1C–hsa-miR-708-3p–JARID2 + first time. By integrating evidence from genetic, neurophenotypic, perspectives, expanded comprehension pathological mechanism provided reference identifying reliable objective biomarkers

Language: Английский

Citations

0