Aregs-IGFBP3-mediated SMC-like cells apoptosis impairs beige adipocytes formation in aged mice DOI Creative Commons
Shifeng Wang, Yuanxu Cui, Limei Wang

et al.

Molecular Metabolism, Journal Year: 2025, Volume and Issue: 95, P. 102125 - 102125

Published: March 19, 2025

Aging is associated with a decline in the browning capacity of white adipose tissue (WAT), contributing to metabolic dysfunction. Beige adipocytes, which dissipate excess energy as heat, are key feature this process. In study, we investigate role stem and progenitor cells (ASPCs), specifically Aregs (CD142+) subpopulation, regulating beige adipocyte formation aged mice under cold stimulation. Our findings reveal that significantly increase subcutaneous WAT (sWAT) following exposure. We further demonstrate secrete insulin-like growth factor binding protein 3 (IGFBP3), appears play pivotal cross-talk between adipogenesis-regulatory (Aregs) smooth muscle cell-like (SMC-like) cells, thereby leading inhibition adipocytes formation. Functional enrichment analysis highlighted activation TGFβ, MAPK p53 signaling pathways SMC-like all known induce cell apoptosis fibrosis. Moreover, IGFBP3 was found interact receptors molecules, including Egfr, Irf1 Cdkn1a, enhancing their apoptosis. Co-culture experiments confirmed suppressed corroborating its impairing browning. Overall, our study provides novel insights into molecular mechanisms by contribute age-related These suggest potential therapeutic targets for reversing impaired aging related disorders.

Language: Английский

Targeting sirtuins for cancer therapy: epigenetics modifications and beyond DOI Creative Commons
Hui Shen,

Xinyi Qi,

Yue Hu

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(17), P. 6726 - 6767

Published: Jan. 1, 2024

Sirtuins (SIRTs) are well-known as nicotinic adenine dinucleotide

Language: Английский

Citations

9
Risk Allele rs117026326‐Mediated Alternative Splicing of GTF2I Promotes B Cell Proliferation in Primary Sjögren’s Syndrome DOI Creative Commons
Chaowen Luo,

Chaofeng Lian,

Jinlei Sun

et al.

Journal of Immunology Research, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Objectives: Primary Sjögren’s syndrome (pSS) is associated with a risk allele T of rs117026326 located at potential splicing enhancer within the intronic region general transcription factor II‐I (GTF2I). This study aimed to explore rs117026326‐regulated alternative GTF2I and its role in B cell overactivation pSS. Methods: isoform expressions genotypes pSS peripheral blood mononuclear cells (PBMCs) were examined using quantitative PCR Sanger sequencing, respectively. GTF2IΔ was overexpressed cells, macrophages plasmid transfection. Proliferation determined Cell Counting Kit‐8 (CCK8) assay. CD4 + differentiation inspected flow cytometry. Proinflammatory cytokine production investigated PCR. c‐FOS expression GTF2IΔ‐transfected tested by PCR, proliferation treated siRNA or inhibitor interrogated CCK8 Results: patients expressed higher levels GTF2Iζ isoforms. correlated serum immunoglobulin G (IgG). promoted upregulated expression. Knocking down inhibition reversed driven GTF2IΔ. Conclusion: modulates upregulates isoform, which promotes through enhancing binding c‐FOS.

Language: Английский

Citations

0
Aregs-IGFBP3-mediated SMC-like cells apoptosis impairs beige adipocytes formation in aged mice DOI Creative Commons
Shifeng Wang, Yuanxu Cui, Limei Wang

et al.

Molecular Metabolism, Journal Year: 2025, Volume and Issue: 95, P. 102125 - 102125

Published: March 19, 2025

Aging is associated with a decline in the browning capacity of white adipose tissue (WAT), contributing to metabolic dysfunction. Beige adipocytes, which dissipate excess energy as heat, are key feature this process. In study, we investigate role stem and progenitor cells (ASPCs), specifically Aregs (CD142+) subpopulation, regulating beige adipocyte formation aged mice under cold stimulation. Our findings reveal that significantly increase subcutaneous WAT (sWAT) following exposure. We further demonstrate secrete insulin-like growth factor binding protein 3 (IGFBP3), appears play pivotal cross-talk between adipogenesis-regulatory (Aregs) smooth muscle cell-like (SMC-like) cells, thereby leading inhibition adipocytes formation. Functional enrichment analysis highlighted activation TGFβ, MAPK p53 signaling pathways SMC-like all known induce cell apoptosis fibrosis. Moreover, IGFBP3 was found interact receptors molecules, including Egfr, Irf1 Cdkn1a, enhancing their apoptosis. Co-culture experiments confirmed suppressed corroborating its impairing browning. Overall, our study provides novel insights into molecular mechanisms by contribute age-related These suggest potential therapeutic targets for reversing impaired aging related disorders.

Language: Английский

Citations

0